CAJ | 학술논문

在此前曾提出过一种新型二维折射率探测方法--并行扫描光谱表面等离子体共振(surface plas-mon resonance,SPR)成像方法.在这种方法中,使用线形光照明,CCD得到的图像包含SPR光谱信息和一维窄间信息,进而通过计算可得到折射率的一维分布信息.通过一维扫描,就能够得到整个被扫描区域内的折射率二维分布信息.该方法具有高灵敏、高通量的优点,适合微阵列(microarray)的检测.并完善了这种方法的数据处理过程,使用空气折射率作为参考,消除了无法精确控制入射角的难题.使用该方法对手工点制的军团菌mip DNA探针微阵列进行了检测,证明了这种方法高灵敏无标记地探测微阵列的可行性.我们得到的军团菌mip DNA探针制备浓度与其等效折射率的关系,这对基于SPR的微阵列技术的发展有着重要的参考意义.
재차전증제출과일충신형이유절사솔탐측방법--병행소묘광보표면등리자체공진(surface plas-mon resonance,SPR)성상방법.재저충방법중,사용선형광조명,CCD득도적도상포함SPR광보신식화일유착간신식,진이통과계산가득도절사솔적일유분포신식.통과일유소묘,취능구득도정개피소묘구역내적절사솔이유분포신식.해방법구유고령민、고통량적우점,괄합미진렬(microarray)적검측.병완선료저충방법적수거처리과정,사용공기절사솔작위삼고,소제료무법정학공제입사각적난제.사용해방법대수공점제적군단균mip DNA탐침미진렬진행료검측,증명료저충방법고령민무표기지탐측미진렬적가행성.아문득도적군단균mip DNA탐침제비농도여기등효절사솔적관계,저대기우SPR적미진렬기술적발전유착중요적삼고의의.
The authors have previously proposed a novel refractive index two-dimensional sensing technique named "parallel scan spectral surface plasmon resonance imaging". In the technique, with a line-shaped light illumination, an image acquired with CCD detector could provide both SPR wavelength information and one-dimensional spatial distribution, and then provide one-dimensional distribution of refractive index with further calculatioa Thus, two-dimensional distribution of refractive index of the entire sensing area can be obtained with one-dimensional optical line parallel scaa The technique offers advantages of both high sensitivity and high throughput, and could have potential applications in microarray analysis. In the present paper, the authors improve the data processing methods of the technique. The authors use the refractive index of air as a reference to get over the problem of precision of the incident angle. The authors also sense a manually dotted Legionella pneumophila mip DNA probe array with this technique and prove the feasibility of sensing microarrays by this highly sensitive and label-free technique. The relation between the equivalent refractive indices and the concentrations of the dotted Legionella pneumophila mip DNA probes is obtained, which has important reference value for further study.