基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2010年
2期
179-184
,共6页
王霜%刘晓力%原少斐%杜红延
王霜%劉曉力%原少斐%杜紅延
왕상%류효력%원소비%두홍연
stalhmin%1单克隆抗体%长春新碱%白血病%增殖%凋亡
stalhmin%1單剋隆抗體%長春新堿%白血病%增殖%凋亡
stalhmin%1단극륭항체%장춘신감%백혈병%증식%조망
monoclonal antibodies against stathmin 1%vinblastine%leukemia%proliferation%apoptosis
目的 探讨抗人stathmin 1单克隆抗体、长春新碱分别单用或联用对慢性髓系白血病细胞株(K562)细胞的生长抑制及促凋亡作用.方法 以不同浓度的抗人stathmin 1单克隆抗体、长春新碱分别和联合用药组,另设对照组,分别作用于K562细胞24、48、72和96 h,MTT法检测细胞的增殖,HoechsG3342染色观察细胞凋亡的情况,用流式细胞仪通过Annexin V/PI双染检测细胞凋亡率.结果 抗人stathmin 1单克降抗体、长春新碱分别单用与联用均能呈剂量-时间依赖抑制K562细胞增殖,联用组细胞抑制率较单用组明显增高(P<0.05),二者联用有交互效应(P<0.05).各实验组细胞出现核固缩、凝集和胞质减少以及凋亡小体的出现,均能诱导K562细胞凋亡,联用组作用更为明显(P<0.05).结论 抗人stathmin 1单克隆抗体、长春新碱分别使用与联合应用均能抑制髓系白血病K562细胞增殖,诱导其凋亡;且两药联合作用于K562细胞具有协同作用.
目的 探討抗人stathmin 1單剋隆抗體、長春新堿分彆單用或聯用對慢性髓繫白血病細胞株(K562)細胞的生長抑製及促凋亡作用.方法 以不同濃度的抗人stathmin 1單剋隆抗體、長春新堿分彆和聯閤用藥組,另設對照組,分彆作用于K562細胞24、48、72和96 h,MTT法檢測細胞的增殖,HoechsG3342染色觀察細胞凋亡的情況,用流式細胞儀通過Annexin V/PI雙染檢測細胞凋亡率.結果 抗人stathmin 1單剋降抗體、長春新堿分彆單用與聯用均能呈劑量-時間依賴抑製K562細胞增殖,聯用組細胞抑製率較單用組明顯增高(P<0.05),二者聯用有交互效應(P<0.05).各實驗組細胞齣現覈固縮、凝集和胞質減少以及凋亡小體的齣現,均能誘導K562細胞凋亡,聯用組作用更為明顯(P<0.05).結論 抗人stathmin 1單剋隆抗體、長春新堿分彆使用與聯閤應用均能抑製髓繫白血病K562細胞增殖,誘導其凋亡;且兩藥聯閤作用于K562細胞具有協同作用.
목적 탐토항인stathmin 1단극륭항체、장춘신감분별단용혹련용대만성수계백혈병세포주(K562)세포적생장억제급촉조망작용.방법 이불동농도적항인stathmin 1단극륭항체、장춘신감분별화연합용약조,령설대조조,분별작용우K562세포24、48、72화96 h,MTT법검측세포적증식,HoechsG3342염색관찰세포조망적정황,용류식세포의통과Annexin V/PI쌍염검측세포조망솔.결과 항인stathmin 1단극강항체、장춘신감분별단용여련용균능정제량-시간의뢰억제K562세포증식,련용조세포억제솔교단용조명현증고(P<0.05),이자련용유교호효응(P<0.05).각실험조세포출현핵고축、응집화포질감소이급조망소체적출현,균능유도K562세포조망,련용조작용경위명현(P<0.05).결론 항인stathmin 1단극륭항체、장춘신감분별사용여연합응용균능억제수계백혈병K562세포증식,유도기조망;차량약연합작용우K562세포구유협동작용.
Objective To investigate the effects of monoclonal antibody against stathmin 1 in combination with vinblastine on the proliferation of K562 cells. Methods K562 cells were treated with monoclonal antibody against stathmin 1, vinblastine alone or with their combination, 24, 48, 72, 96 hours later, inhabitation rate was studied by MTT assay;The apoptosis was analyzed by invert microscope and flow cytometry with Annexin V/PI. Results The quantity decreased and shape, size changed after treatment with different concentration of experimental groups. Monoclonal antibodies against stathmin 1 and vinblastine used alone or in combination both inhibited the proliferation of K562 cells,the inhibition ratio of their combination is higher (P <0. 05) ,and a synergistic effect of the two agents was noted in their combined action ( P < 0. 05 ). Combined treatment of the cells resulted in significantly higher apoptsis rate than that in the other groups (P <0. 05). Conclusion Monoclonal antibody against stathmin 1 and vinblastine used alone or in combination both can inhibite proliferation of K562 cells and induce apoptsis. A synergistic effect is observed between the monoclonal antibodies against stathmin 1 and vinblastine in their inhibition of K562 cell proliferation.
