CAJ | 학술논문

目的:诱变筛选阿魏菇多糖高产菌,探索食用菌的离子束和激光复合诱变育种方法.方法:尝试用阿魏菇菌丝单细胞为靶材,以离子束注人和激光辐照为复合诱变手段,采用营养缺陷型筛选办法定性初筛,摇瓶发酵定量复筛.结果:通过离子束注入诱变,获得了2株菌丝体多糖产量分别达到551.80mg/L和659.46mg/L、较l号出发菌株提高了46.5%和75.2%的多糖高产菌PFPH-1和PFPH-2;在此基础上,以激光辐照为复合诱变手段,获得了1株菌丝体多糖产量达到762.50 mg/L、较出发菌株PFPH-2提高了15.63%的多糖高产菌PFPH-3.结论:离子束和激光复合诱变育种方法在改良阿魏菇多糖高产性状方面诱变功效显著.
목적:유변사선아위고다당고산균,탐색식용균적리자속화격광복합유변육충방법.방법:상시용아위고균사단세포위파재,이리자속주인화격광복조위복합유변수단,채용영양결함형사선판법정성초사,요병발효정량복사.결과:통과리자속주입유변,획득료2주균사체다당산량분별체도551.80mg/L화659.46mg/L、교l호출발균주제고료46.5%화75.2%적다당고산균PFPH-1화PFPH-2;재차기출상,이격광복조위복합유변수단,획득료1주균사체다당산량체도762.50 mg/L、교출발균주PFPH-2제고료15.63%적다당고산균PFPH-3.결론:리자속화격광복합유변육충방법재개량아위고다당고산성상방면유변공효현저.
Objective:To obtain Pleurotus polysaccharide high - yield strains and explore an ion beam and laser composite mutagenesis breeding method for edible fungus. Method:Mycelium mono - cells of Asafoetida mushroom were treated tentatively by ion beam and laser composite mutagenesis through fermentation quantitative screening after auxotrophy qualitative primary screening. Result:Two polysaccharide high - y ield strains, PFPH - 1 and PFPH - 2, were selected by ion beam implantation. The polysaccharide yield of these mutants was 551.80mg/L and 659.46mg/L, which were increased by 46.5% and 75.2% compared to that of initial strain No.1. On this basis, a polysaccharid e high - yield strain named PFPH - 3 was obtained by laser eradiation as composite mutagenesis method. The polysaccharide was 762.50mg/L, which was increased by 15.63% compared to that of initial strain PFPH -2. Conclusion: In this study, the mutagenic effect was significant that ion beam and laser composite mutagenesis breeding method was used in improving Pleurotus polysaccharide yield.