CAJ | 학술논문

[目的]研究自然条件下选育的3种不同耐寒番茄品系的耐寒指标及其生理特性.[方法]以3-4周的番茄幼苗为材料,通过实验室冷处理模拟外界环境条件,鉴定植株的存活及生长状况;研究植株离体叶片冷处理恢复后的形态变化并测定相对电导率,鉴定不同品系番茄植株的耐寒程度,分析电导率与存活率的相关性;测定冷胁迫条件下番茄植株体内可溶性糖和脯氨酸含量变化.[结果]正常培养条件下,耐寒品系番茄生长4周的植株形态矮小,中等耐寒品系番茄形态略高,不耐寒品系最为高壮.-4℃条件下处理6 h后,耐寒品系、中等耐寒品系、不耐寒品系番茄,平均存活率分别达63.33%、44.57%、1.23%.番茄离体叶片-4℃冷处理后,耐寒品系叶片卷缩程度较小,叶片颜色旱碧绿色;中等耐寒品系叶片颜色呈深绿色且卷缩程度较大;不耐寒品系叶片颜色呈深黑色,卷缩程度最大.耐寒品系90%以上的相对电导率在42.62%以下,不耐寒品系90%以上的电导率在62.67%以上,中等耐寒品系90%以上的电导率约为52.05%.不同品系番茄植株体内可溶性糖含量变化差异显著,脯氮酸含量变化差异不显著.[结论]冷处理条件下电导率与存活率存在一定相关性,植株耐寒性与可溶性糖含量的积累有关.
[목적]연구자연조건하선육적3충불동내한번가품계적내한지표급기생리특성.[방법]이3-4주적번가유묘위재료,통과실험실랭처리모의외계배경조건,감정식주적존활급생장상황;연구식주리체협편랭처리회복후적형태변화병측정상대전도솔,감정불동품계번가식주적내한정도,분석전도솔여존활솔적상관성;측정랭협박조건하번가식주체내가용성당화포안산함량변화.[결과]정상배양조건하,내한품계번가생장4주적식주형태왜소,중등내한품계번가형태략고,불내한품계최위고장.-4℃조건하처리6 h후,내한품계、중등내한품계、불내한품계번가,평균존활솔분별체63.33%、44.57%、1.23%.번가리체협편-4℃랭처리후,내한품계협편권축정도교소,협편안색한벽록색;중등내한품계협편안색정심록색차권축정도교대;불내한품계협편안색정심흑색,권축정도최대.내한품계90%이상적상대전도솔재42.62%이하,불내한품계90%이상적전도솔재62.67%이상,중등내한품계90%이상적전도솔약위52.05%.불동품계번가식주체내가용성당함량변화차이현저,포담산함량변화차이불현저.[결론]랭처리조건하전도솔여존활솔존재일정상관성,식주내한성여가용성당함량적적루유관.
[Objective]The aim was to study cold-resistant indexes and physiological characters of three different cold-resisitant tomato strains selected under natural conditions.[Method]With 3-4 weeks tomato seedlings as tested materials,through simulating external environment conditions by the cold treatment in lab,the survival ratio and growth status of seedlings were identified;by studying the morphological change of in vitro leaves after cold treatment and determining the relative conductivity,the cold-resistant degree of different tomato strains was identified and the correlation between conductivity and survival rate was analyzed;the variation of soluble sugar and praline content in plant of tomato was determined under the condition of cold stress.[Result]The results showed that leaf morphological changes,size of conductivity and growth status of different tomato strains after cold treatment have significant differences;the variation of soluble sugar contents in plant has significant differences,while the variation of proline content has no significant differences.[Conclusion]There is a certain correlation between conductivity and survival rate under cold treatment conditions,and plant cold tolerance is related to accumulation of soluble sugar content.