CAJ | 학술논문

目的通过哈弗氏系统改建的实验研究,探索一种新的能更好地促进骨损伤尽快修复的引导组织再生性生物膜材料.方法以家兔为研究对象,在其双侧胫骨中上三分之一交界处及下颌角前切迹处形成急性骨创,实验侧骨创处覆盖海藻酸钙膜,对照侧覆盖胶元膜或不覆盖膜材料,分别在术后1、2、4、6、8周时取出颌骨与胫骨,进行大体观察、组织学观察、TGF-β与VEGF抗体免疫组化染色并计量观察.结果术后6周内实验组哈弗氏管比对照侧出现早而多,骨再生快;前者异物巨噬细胞反应程度轻,后者反应程度重;术后4周内实验侧TGF-β与VEGF含量显著高于对照组(P＜0.05),而且具有时空性.结论哈弗氏系统的改建和骨诱导因子的含量与生物膜材料引导组织再生的能力和效果直接相关.
목적 통과합불씨계통개건적실험연구,탐색일충신적능경호지촉진골손상진쾌수복적인도조직재생성생물막재료.방법 이가토위연구대상,재기쌍측경골중상삼분지일교계처급하합각전절적처형성급성골창,실험측골창처복개해조산개막,대조측복개효원막혹불복개막재료,분별재술후1、2、4、6、8주시취출합골여경골,진행대체관찰、조직학관찰、TGF-β여VEGF항체면역조화염색병계량관찰.결과 술후6주내실험조합불씨관비대조측출현조이다,골재생쾌;전자이물거서세포반응정도경,후자반응정도중;술후4주내실험측TGF-β여VEGF함량현저고우대조조(P＜0.05),이차구유시공성.결론 합불씨계통적개건화골유도인자적함량여생물막재료인도조직재생적능력화효과직접상관.
Objective To search a better novae biomaterial applied to guide bone regeneration for promoting the healing of acute bone trauma by the experimental study of Haversian remodeling.Method Circular bone defects of 5mm diameter were created in tibias and the corners of mandibles in 36 rabbits.The defects were covered with calcium alginate film (CAF) in the experimental group, collagen membrane (CM)and no membrane (blank) in the control groups respectively. Healing conditions were analyzed using gross inspection, and histological and immunohistochemical studies after 1, 2, 4, 6 and 8 weeks respectively.Results The experimental group appeared more and earlier Haversian remodeling with osteoinductive factors leading to better bone regeneration. The control groups showed more macrophages with CM absorbed slowly, weak and delayed Haversian remodeling, and less osteoinductive factors ( P ＜ 0.05) in the early stage.Conclusions Calcium alginate film, as a relatively cheaper biomaterial, provided better effect than the collagen membrane on guided bone regeneration, because of its better Haversian remodeling and more content of osteoinductive factors shown in this experiment.