中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2008年
11期
14-16
,共3页
贾友鹏%王忠裕%张利%吴园园
賈友鵬%王忠裕%張利%吳園園
가우붕%왕충유%장리%오완완
结肠肿瘤%肿瘤坏死因子α%角蛋白质类%逆转录聚合酶链反应
結腸腫瘤%腫瘤壞死因子α%角蛋白質類%逆轉錄聚閤酶鏈反應
결장종류%종류배사인자α%각단백질류%역전록취합매련반응
Colonic neoplasms%Tumor necrosis factor-alpha%Keratins%Reverse transcrip-tase polymerase chain reaction
目的 研究TNF-α和细胞角蛋白20(CK20)在结肠癌患者组织中的表达和相关性,及与肿瘤生物学行为的关系.方法 选取手术切除并经病理检查证实为结肠癌患者30例,采用逆转录聚合酶链反应(RT-PCR)法,检测结肠癌患者癌组织、癌旁组织、切缘组织中TNF-α mRNA和CK20 mRNA的表达情况.结果 癌组织、癌旁组织、切缘组织中TNF-α mRNA阳性表达率分别为70.0%、43.3%、20.0%,三者之间比较及每两者之间比较差异均有统计学意义(P＜0.01);CK20 mRNA阳性表达率分别为63.3%、33.3%、16.7%,三者之间比较差异有统计学意义(P＜0.01),但在癌旁组织和切缘组织之间表达差异则无统计学意义(P＞0.05).TNF-α mRNA和CK20 mRNA在三种组织中的表达具有良好的相关性.TNF-α mRNA和CK20 mRNA与结肠癌分化程度无关(P＞0.05),但TNF-α mRNA与结肠癌Dukes分期、侵犯肠壁范围密切相关(P＜0.05).结论 TNF-α mRNA可以作为反映结肠癌浸润、侵袭能力的客观指标.
目的 研究TNF-α和細胞角蛋白20(CK20)在結腸癌患者組織中的錶達和相關性,及與腫瘤生物學行為的關繫.方法 選取手術切除併經病理檢查證實為結腸癌患者30例,採用逆轉錄聚閤酶鏈反應(RT-PCR)法,檢測結腸癌患者癌組織、癌徬組織、切緣組織中TNF-α mRNA和CK20 mRNA的錶達情況.結果 癌組織、癌徬組織、切緣組織中TNF-α mRNA暘性錶達率分彆為70.0%、43.3%、20.0%,三者之間比較及每兩者之間比較差異均有統計學意義(P＜0.01);CK20 mRNA暘性錶達率分彆為63.3%、33.3%、16.7%,三者之間比較差異有統計學意義(P＜0.01),但在癌徬組織和切緣組織之間錶達差異則無統計學意義(P＞0.05).TNF-α mRNA和CK20 mRNA在三種組織中的錶達具有良好的相關性.TNF-α mRNA和CK20 mRNA與結腸癌分化程度無關(P＞0.05),但TNF-α mRNA與結腸癌Dukes分期、侵犯腸壁範圍密切相關(P＜0.05).結論 TNF-α mRNA可以作為反映結腸癌浸潤、侵襲能力的客觀指標.
목적 연구TNF-α화세포각단백20(CK20)재결장암환자조직중적표체화상관성,급여종류생물학행위적관계.방법 선취수술절제병경병리검사증실위결장암환자30례,채용역전록취합매련반응(RT-PCR)법,검측결장암환자암조직、암방조직、절연조직중TNF-α mRNA화CK20 mRNA적표체정황.결과 암조직、암방조직、절연조직중TNF-α mRNA양성표체솔분별위70.0%、43.3%、20.0%,삼자지간비교급매량자지간비교차이균유통계학의의(P＜0.01);CK20 mRNA양성표체솔분별위63.3%、33.3%、16.7%,삼자지간비교차이유통계학의의(P＜0.01),단재암방조직화절연조직지간표체차이칙무통계학의의(P＞0.05).TNF-α mRNA화CK20 mRNA재삼충조직중적표체구유량호적상관성.TNF-α mRNA화CK20 mRNA여결장암분화정도무관(P＞0.05),단TNF-α mRNA여결장암Dukes분기、침범장벽범위밀절상관(P＜0.05).결론 TNF-α mRNA가이작위반영결장암침윤、침습능력적객관지표.
Objective To investigate the expression ofTNF- α mRNA and cytokeratin 20(CK20)mRNA in different tissue of colonic cancer patients, and the relations between the expression and the classify,invasion, as well as Dukes stage of colonic cancer. Methods RT-PCR method was used to detect the ex-pression of TNF-α mRNA and CK20 mRNA in 30 cases of colonic cancer, included cancer tissue,para-cancer tissue and normal tissue. Results The positive rate of TNF- α mRNA expressions in cancer tissue, para-cancer tissue and normal tissue were 70.0%, 43.3% and 20.0%, and the positive rate of CK20mRNA expressions were 63.3%, 33.3% and 16.7%, there were significant difference among the three tissues(P ＜ 0.01 ). But the expression of CK20 mRNA in para-cancer tissue had no significant difference compared with normal tissue (P＞ 0.05). The expression ofTNF- α mRNA was closely correlated with that of CK20mRNA.TNF- α mRNA and CK20 mRNA showed no significant difference in expressing of colonic cancer tissue (P ＞ 0.05 ), but TNF- α was closely correlated with Duke stage and depth of tumor invasion (P ＜ 0.05 ).Conclusion The expression of TNF- α mRNA is objective indicator associated with the invasion of the colonic cancer.
