CAJ | 학술논문

目的荧光显微镜直视下对比评价携抗P-选择素单抗靶向微泡与同型对照微泡在微循环中的黏附机制及行为方式.方法构建携荧光FITC的抗P-选择素单抗靶向微泡(MBp)和同型对照微泡(MBiso),并随机经静脉注入小鼠提睾肌炎症模型.20倍荧光显微镜直视下观察并记录5min内两种微泡在提睾肌微循环中的黏附情况,并对不同黏附方式的微泡进行计数.应用image-pro-plus分析软件对微泡进行定点追踪,并对其黏附过程中速度的变化进行定量测定.结果荧光显微镜下观察可见MBp组与内皮黏附数量高达(8.4±2.1)个/视野,MBiso组仅为(0.8±0.8)个/视野,两者间差异有统计学意义(P＜0.01);MBp和MBiso组白细胞黏附数量分别为(3.6±0.6)个/视野、(2.2±0.8)个/视野,两者间差异无统计学意义(P＞0.05).微泡黏附过程速度变化曲线显示MBp和MBiso分别以流动速度逐渐和迅速降低两种方式实现对靶组织的黏附.结论 MBp和MBiso具有不同的黏附方式,MBp能更高效、特异地黏附于炎症组织血管内皮上,为评价血管内皮炎症反应或其他组织损伤的应用提供了理论基础.
목적 형광현미경직시하대비평개휴항P-선택소단항파향미포여동형대조미포재미순배중적점부궤제급행위방식.방법 구건휴형광FITC적항P-선택소단항파향미포(MBp)화동형대조미포(MBiso),병수궤경정맥주입소서제고기염증모형.20배형광현미경직시하관찰병기록5min내량충미포재제고기미순배중적점부정황,병대불동점부방식적미포진행계수.응용image-pro-plus분석연건대미포진행정점추종,병대기점부과정중속도적변화진행정량측정.결과 형광현미경하관찰가견MBp조여내피점부수량고체(8.4±2.1)개/시야,MBiso조부위(0.8±0.8)개/시야,량자간차이유통계학의의(P＜0.01);MBp화MBiso조백세포점부수량분별위(3.6±0.6)개/시야、(2.2±0.8)개/시야,량자간차이무통계학의의(P＞0.05).미포점부과정속도변화곡선현시MBp화MBiso분별이류동속도축점화신속강저량충방식실현대파조직적점부.결론 MBp화MBiso구유불동적점부방식,MBp능경고효、특이지점부우염증조직혈관내피상,위평개혈관내피염증반응혹기타조직손상적응용제공료이론기출.
Objective To evaluate the adhesion mechanisms and microvascular behaviors of P-selectin targeted microbubbles and isotype control microbubbles.Methods The targeted microbubbles with FITC fluorescence and antibodies of P-selectin (MBp), and the isotype control microbubbles with FITC fluorescence and isotype antibodies(MBiso) were constructed.MBp and MBiso were injected intravenously in random to wild-type mice with TNF-α stimulus.The mechanisms and behaviors of MBp and MBiso in cremasteric venules were assessed by fluorescence microscopy.And then velocity variation of ultrasound microbubbles in microvascular was analyzed by tracking fixed-point technology of image-pro-plus software.Results The retention of microbubbles directly to venular endothelium amounted to (8.4 ± 2.1 )/view in MBp-group,while it was only (0.8 ± 0.8)/view in MBiso-group.Difference was evident between of the two groups( P ＜0.01 ).The numbers of MBp and MBiso attachment to activated leukocytes were (3.6± 0.6)/view and (2.2± 0.8)/view separately.There was no obvious difference between the two groups( P ＞0.05).The variation curves of velocity in MBp and MBiso targeting to venular endothelium were different.The flowing rate decreased gradually in MBp-group,while it decreased rapidly in MBiso-group.Conclusions MBp and MBiso have different behaviors targeting to venular endothelium, MBp could adhere to vascular endothelial efficiently and specifically, these results suggest MBp may be used to assess inflammation of vascular endothelial or other tissue injury effectively.