CAJ | 학술논문

目的观察并比较不同时间给予大蒜油对四氯化碳(carbon tetrachloride,CCl_4)引起的急性化学性肝损伤的保护效果.方法实验分为4个预防组和2个治疗组,其中每组又分为阴性对照组、溶剂对照组(玉米油)、CCl_4模型组、大蒜油低(25mg/kg)、中(50mg/kg)、高(100mg/kg)剂量组,每组15只雄性昆明种小鼠.预防组给药方案为预防1组,提前灌胃大蒜油30 d;预防2组,提前灌胃大蒜油5d,均为1次/d(10ml/kg),末次给大蒜油后4 h灌胃1次CCl_4(80mg/kg,10ml/kg);预防3组,提前2 h灌胃1次大蒜油;预防4组,灌胃CCl_4的同时给予1次大蒜油.治疗1组为灌胃CCl_4后2 h灌胃1次大蒜油,治疗2组为灌胃CCl_4后给予5 d大蒜油(1次/d,首次给药时间为CCl_4染毒后2h).溶剂对照组灌胃等体积的玉米油.模型组灌胃1次CCl_4给药时间与各大蒜油组相同.末次给药(CCl_4或大蒜油)后24 h,取血测定各组小鼠血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活力,取肝脏,称重,并计算肝脏系数,同时取肝左叶进行病理组织学检查.结果与阴性对照组相比,CCl_4模型组肝脏系数明显增加,血清中ALT、AST活力明显升高,差异有统计学意义(P<0.01).与CCl_4模型组相比,预防组各组的大蒜油低、中、高剂量组肝脏系数明显减小,血清中ALIT、AST活力明显降低,差异有统计学意义(P<0.01),并有剂量-反应关系;同时大蒜油可有效减轻CCl_4引起的细胞坏死、气球样变等肝细胞损伤.不同预防组大蒜油的保护效果相比,预防3组效果较好.但治疗1组各项指标无明显改善,治疗2组已基本恢复正常.结论大蒜油能预防CCl_4引起的急性肝损伤,并且提前2 h给药预防效果较好.
목적 관찰병비교불동시간급여대산유대사록화탄(carbon tetrachloride,CCl_4)인기적급성화학성간손상적보호효과.방법 실험분위4개예방조화2개치료조,기중매조우분위음성대조조、용제대조조(옥미유)、CCl_4모형조、대산유저(25mg/kg)、중(50mg/kg)、고(100mg/kg)제량조,매조15지웅성곤명충소서.예방조급약방안위예방1조,제전관위대산유30 d;예방2조,제전관위대산유5d,균위1차/d(10ml/kg),말차급대산유후4 h관위1차CCl_4(80mg/kg,10ml/kg);예방3조,제전2 h관위1차대산유;예방4조,관위CCl_4적동시급여1차대산유.치료1조위관위CCl_4후2 h관위1차대산유,치료2조위관위CCl_4후급여5 d대산유(1차/d,수차급약시간위CCl_4염독후2h).용제대조조관위등체적적옥미유.모형조관위1차CCl_4급약시간여각대산유조상동.말차급약(CCl_4혹대산유)후24 h,취혈측정각조소서혈청중병안산전안매(ALT)화천동안산전안매(AST)적활력,취간장,칭중,병계산간장계수,동시취간좌협진행병리조직학검사.결과 여음성대조조상비,CCl_4모형조간장계수명현증가,혈청중ALT、AST활력명현승고,차이유통계학의의(P<0.01).여CCl_4모형조상비,예방조각조적대산유저、중、고제량조간장계수명현감소,혈청중ALIT、AST활력명현강저,차이유통계학의의(P<0.01),병유제량-반응관계;동시대산유가유효감경CCl_4인기적세포배사、기구양변등간세포손상.불동예방조대산유적보호효과상비,예방3조효과교호.단치료1조각항지표무명현개선,치료2조이기본회복정상.결론 대산유능예방CCl_4인기적급성간손상,병차제전2 h급약예방효과교호.
Objective To observe and compare the protective effect of garlic oil against carbon tetra-chloride (CCl_4)-induced acute liver injury.Methods The experiments include 4 preventive groups and 2 therapeutic groups. In every preventive and therapeutic group, the mice were randomized into 6 groups with 15 each, including one negative control group, one solvent control group, one CC1_4 model group and 3 garlic oil groups (25, 50, and 100 mg/kg body weight). Before given a single gavage of CCl_4(80 mg/kg), the mice were pretreated with garlic oil by gavage in preventive group 1 (30 days, once daily), preventive group 2 (5 days, once daily), preventive group 3 (ahead of 2 h, once), preventive group 4 (immediately, once) or the vehicle (corn oil, 10 ml/kg) in solvent control group. In therapeutic groups, the mice were gavaged garlic oil 2 h (once, in therapeutic 1) or for 5 days (once daily, in therapeutic 2) after administration CCl_4. After 24 h of the last administration, blood was collected and centrifuged at 2500 r/min at 4?for 10 min, and serum was removed to measure ALT and AST activities. The liver was dissected, weighed to calculate the liver coefficient (relative liver weight). At the same time, the liver samples were studied by histological examinations.Results Compared with negative group, the liver coefficient and the activities of ALT and AST in serum of model group were increased remarkably (P<0.01). Compared with CCl_4 model group, the liver coefficient and the activities of ALT and AST in serum were decreased significantly (P<0.01) by garlic oil dose-dependently in each preventive group. Simultaneously, histological assessment showed that garlic oil effectively alleviated hepatocyte injuries induced by CCl_4. Comparing the preventive effects of garlic oil in every group, it was better in preventive group 3 than others. However, all indexes and histological examinations in therapeutic group 1 did not show the difference with those of CCl_4 model group. In therapeutic group 2, all indexes recovered after 5 d of CCl_4 administration. Conclusions Garlic oil can prevent acute liver injury induced by CCl_4 and the effect is better in ahead of 2 h group than others.