中华精神科杂志
中華精神科雜誌
중화정신과잡지
CHINESE JOURNA OF PSYCHIATRY
2011年
2期
70-74
,共5页
杨丽%邵静茹%阮冶%吴艳瑞%周利国%张勇辉%伍星%郑冰蓉%肖春杰
楊麗%邵靜茹%阮冶%吳豔瑞%週利國%張勇輝%伍星%鄭冰蓉%肖春傑
양려%소정여%원야%오염서%주리국%장용휘%오성%정빙용%초춘걸
精神分裂症%多态性,单核苷酸%D-氨基酸氧化酶激活剂基因
精神分裂癥%多態性,單覈苷痠%D-氨基痠氧化酶激活劑基因
정신분렬증%다태성,단핵감산%D-안기산양화매격활제기인
Schizophrenia%Polymorphisms,single nucleotide%D-amino acid oxidase activator gene
目的 探讨D-氨基酸氧化酶激活剂(D-amino acid oxidase activator,DAOA)基因rs2391191、rs947267、rs778294多态性与云南汉族人群精神分裂症发病的相关性.方法 采用聚合酶链式反应-限制性片段长度多态(PCR-RFLP)方法,对276例精神分裂症患者(患者组)和312名健康对照(对照组)DAOA基因rs2391191、rs947267和rs778294 3个位点的多态进行检测,在混合群体和性别分层群体中进行多态性与精神分裂症关联分析.结果 (1)患者组和对照组rs2391191、rs947267、rs778294 3个位点等位基因频率分布的差异无统计学意义(P>0.05).(2)患者组男性和对照组男性rs2391191等位基因的频率分布差异无统计学意义(P>0.05);患者组男性rs947267 C和rs778294 A等位基因的频率分别为0.406,0.184,对照组男性分别为0.327,0103,患者组高于对照组[校正相对风险度(OR)=1.41,95%可信区间(CI)=1.00～1.99,P=0.049;OR=1.91,95%CI=l.21～3.20,P=0.006].(3)对照组男性单倍型GAA的频率显著高于患者组男性(P=0.008).结论 DAOA基因的rs947267、rs778294位点可能与男性精神分裂症发病存在相关性,rs947267 C等位基因和rs778294 A等位基因可能是男性精神分裂症发病的危险因子;单倍型GAA可能是男性精神分裂症的保护性单倍型.
目的 探討D-氨基痠氧化酶激活劑(D-amino acid oxidase activator,DAOA)基因rs2391191、rs947267、rs778294多態性與雲南漢族人群精神分裂癥髮病的相關性.方法 採用聚閤酶鏈式反應-限製性片段長度多態(PCR-RFLP)方法,對276例精神分裂癥患者(患者組)和312名健康對照(對照組)DAOA基因rs2391191、rs947267和rs778294 3箇位點的多態進行檢測,在混閤群體和性彆分層群體中進行多態性與精神分裂癥關聯分析.結果 (1)患者組和對照組rs2391191、rs947267、rs778294 3箇位點等位基因頻率分佈的差異無統計學意義(P>0.05).(2)患者組男性和對照組男性rs2391191等位基因的頻率分佈差異無統計學意義(P>0.05);患者組男性rs947267 C和rs778294 A等位基因的頻率分彆為0.406,0.184,對照組男性分彆為0.327,0103,患者組高于對照組[校正相對風險度(OR)=1.41,95%可信區間(CI)=1.00～1.99,P=0.049;OR=1.91,95%CI=l.21～3.20,P=0.006].(3)對照組男性單倍型GAA的頻率顯著高于患者組男性(P=0.008).結論 DAOA基因的rs947267、rs778294位點可能與男性精神分裂癥髮病存在相關性,rs947267 C等位基因和rs778294 A等位基因可能是男性精神分裂癥髮病的危險因子;單倍型GAA可能是男性精神分裂癥的保護性單倍型.
목적 탐토D-안기산양화매격활제(D-amino acid oxidase activator,DAOA)기인rs2391191、rs947267、rs778294다태성여운남한족인군정신분렬증발병적상관성.방법 채용취합매련식반응-한제성편단장도다태(PCR-RFLP)방법,대276례정신분렬증환자(환자조)화312명건강대조(대조조)DAOA기인rs2391191、rs947267화rs778294 3개위점적다태진행검측,재혼합군체화성별분층군체중진행다태성여정신분렬증관련분석.결과 (1)환자조화대조조rs2391191、rs947267、rs778294 3개위점등위기인빈솔분포적차이무통계학의의(P>0.05).(2)환자조남성화대조조남성rs2391191등위기인적빈솔분포차이무통계학의의(P>0.05);환자조남성rs947267 C화rs778294 A등위기인적빈솔분별위0.406,0.184,대조조남성분별위0.327,0103,환자조고우대조조[교정상대풍험도(OR)=1.41,95%가신구간(CI)=1.00～1.99,P=0.049;OR=1.91,95%CI=l.21～3.20,P=0.006].(3)대조조남성단배형GAA적빈솔현저고우환자조남성(P=0.008).결론 DAOA기인적rs947267、rs778294위점가능여남성정신분렬증발병존재상관성,rs947267 C등위기인화rs778294 A등위기인가능시남성정신분렬증발병적위험인자;단배형GAA가능시남성정신분렬증적보호성단배형.
Objective To investigate the association between single nucleotide polymorphisms (SNPs) of D-amino acid oxidase activator (DAOA) gene and schizophrenia in Han population from Yunnan Province.Methods Two hundred and seventy-six schizophrenic patients and 312 healthy controls were recruited.Genotyping was performed using polymerase chain reaction(PCR)-restriction fragment length polymerase(RFLP).Odds ratios(ORs)and 95%confidence intervals(CIs)were calculated to evaluate the relationship between the gene polymorphisms and schizophrenia.Results (1)No significant differences of rs2391191,rs947267 and rs778294 allele frequencies were observed between the patients and controls,and the same results existed in the females.(2)In males,there was no significant difference in the allele frequency of rs2391191(P>0.05)between the two groups.The frequencies of rs947267 C allele and rs778294 A allele(0.406 and 0.184,respectively)were higher in patients compared to that in controls (0.327 and 0.103,respectively)[adjusted OR=1.41,95%CI=1.00-1.99,P=0.049;adjusted OR=1.91,95%CI=1.21-3.20,P=0.006].(3)In males,the frequency of haplotype GAA was significantly higher in controls than that in patients(P=0.008).Conclusions The DAOA gene rs947267 and rs778294 polymorphisms may be associated with schizophrenia in males.Rs947267 C allele and rs778294 A allele are possibly risk factors for schizophrenia in Yunnan Han male population.Haplotype GAA may be associated with schizophrenia as a protective factor in males.
