HIV-1包膜蛋白V2区突变对V3区特异的中和抗体的影响
HIV-1포막단백V2구돌변대V3구특이적중화항체적영향
Impact of mutation at V2 of gp120 on V3-specific neutralizing antibodies
  • 万方数据
    中华微生物学和免疫学杂志 중화미생물학화면역학잡지
    CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
    2011年 7期 613-617 ,共5页

    刘沐桑%松下修三%柴田润二%刘维达

    류목상%송하수삼%시전윤이%류유체

    HIV%V2区%V3区%突变%中和抗体 HIV%V2區%V3區%突變%中和抗體
    HIV%V2구%V3구%돌변%중화항체
    HIV%V2 region%V3 region%Mutation%Neutralizing antibody
    目的 研究HIV-1包膜蛋白V2区突变对V3区特异中和抗体与HIV-1中和作用的影响.方法 使用假病毒中和试验测定L175P突变对于V3区中和抗体对HIV-1中和能力的影响,ELISA方法测定上述中和抗体对gp120蛋白单体的结合力.结果 V2区突变L175P可以改变多个V3区特异性中和抗体对病毒的中和能力,但这些中和抗体对gp120蛋白单体的结合力不因V2区突变而改变.结论 V2区的L175P突变改变了gp120蛋白天然三聚体的结构,促进了V3区抗体的中和能力.
    목적 연구HIV-1포막단백V2구돌변대V3구특이중화항체여HIV-1중화작용적영향.방법 사용가병독중화시험측정L175P돌변대우V3구중화항체대HIV-1중화능력적영향,ELISA방법측정상술중화항체대gp120단백단체적결합력.결과 V2구돌변L175P가이개변다개V3구특이성중화항체대병독적중화능력,단저사중화항체대gp120단백단체적결합력불인V2구돌변이개변.결론 V2구적L175P돌변개변료gp120단백천연삼취체적결구,촉진료V3구항체적중화능력.
    Objective To study the impact of V2 mutations on neutralizing ability of HIV-1-specific neutralizing antibodies. Methods We tested the influence of L175P mutation to the neutralizing ability of V3-specific antibodies by pseudotype virus and the binding affinity of those V3-spesific antibodies to gpl20 monomer by ELISA. Results We found L175P mutation changed the neutralizing ability of V3-specific antibodies. However, L175P mutation showed no effects on the binding affinity of these antibodies to gpl20 monomer. Conclusion Our results revealed the L175P mutation at V2 loop changed the natural trimmer structure of gp120 and enhanced the neutralizing ability of V3-specific antibodies.
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