CAJ | 학술논문

目的在分子水平研究胃癌与正常组织的差异基因表达情况.方法 2009年12月至2010年6月收集上海交通大学医学院附属新华医院病例.利用基因芯片技术筛选胃癌与正常胃镜病理标本(各12例)的差异表达基因.反转录(RT)-PCR验证差异基因表达.结果通过t检验和SAM检验筛选出69个上调基因,80个下调基因,这些差异基因与细胞黏附、血管生成、细胞增殖与凋亡等有关.差异基因还与Wnt( 1/151,0.66％)、血管内皮生长因子(VEGF) (2/76,2.63％)等信号通路密切相关.RT-PCR检测胃癌及对照组ATP4A、CLDN10、OLFM4、SAA1和PROK2基因表达(0.94±0.19比4.33±0.39、1.00±0.14比3.04±0.26、5.37±0.30比1.02±0.14、4.37±0.30比0.95±0.29、2.62±0.54比1.35±0.35),结果与基因表达谱数据一致(均P＜0.05).结论基于基因表达谱分析所得的差异基因与胃癌发生发展密切相关.
목적 재분자수평연구위암여정상조직적차이기인표체정황.방법 2009년12월지2010년6월수집상해교통대학의학원부속신화의원병례.이용기인심편기술사선위암여정상위경병리표본(각12례)적차이표체기인.반전록(RT)-PCR험증차이기인표체.결과 통과t검험화SAM검험사선출69개상조기인,80개하조기인,저사차이기인여세포점부、혈관생성、세포증식여조망등유관.차이기인환여Wnt( 1/151,0.66％)、혈관내피생장인자(VEGF) (2/76,2.63％)등신호통로밀절상관.RT-PCR검측위암급대조조ATP4A、CLDN10、OLFM4、SAA1화PROK2기인표체(0.94±0.19비4.33±0.39、1.00±0.14비3.04±0.26、5.37±0.30비1.02±0.14、4.37±0.30비0.95±0.29、2.62±0.54비1.35±0.35),결과여기인표체보수거일치(균P＜0.05).결론 기우기인표체보분석소득적차이기인여위암발생발전밀절상관.
Objective To explore the different gene expressions of normal versus tumor tissues of gastric cancer at molecular levels.Methods Gene chip technology was used to determine the differentially expressed genes between gastric cancer( n =12) and normal tissues( n =12) from December 2009 to June 2010 of Xinhua Hospital of Shanghai Jiaotong University School of Medicine.And reverse transcriptase (RT)-PCR was performed to validate the results of gene chip analysis.Results Sixty-nine up-regulated genes and 80 down-regulated genes were identified by significance analysis of microarrays (SAM).And these genes were correlated with cell adhesion,angiogenesis,cell proliferation and apoptosis,et al.They were also closely correlated with the signaling pathways of Wnt ( 1/151,0.66％ ) and vascular endothelial growth factor (VEGF) (2/76,2.63％ ).The differential expressions of ATP4A,CLDN10,OLFM4,SAA1 and PROK2 were confirmed by RT-PCR ( 0.94 ± 0.19 vs 4.33 ± 0.39,1.00 ± 0.14 vs 3.04 ± 0.26,5.37±0.30 vs 1.02±0.14,4.37 ±0.30 vs 0.95 ±0.29,2.62±0.54 vs 1.35 ±0.35,all P ＜0.05).Conclusion The classifier genes identified in this study may be closely correlated with the carcinogenesis of gastric cancer.