中国血吸虫病防治杂志
中國血吸蟲病防治雜誌
중국혈흡충병방치잡지
CHINESE JOURNAL OF SCHISTOSOMIASIS CONTROL
2009年
6期
511-514
,共4页
王瑞兵%周永华%高庆凤%岳慧%石芳%高琪
王瑞兵%週永華%高慶鳳%嶽慧%石芳%高琪
왕서병%주영화%고경봉%악혜%석방%고기
弓形虫感染%雄性生殖%男性不育%大鼠
弓形蟲感染%雄性生殖%男性不育%大鼠
궁형충감염%웅성생식%남성불육%대서
Toxoplasma gondii infection%Male reproduction%Masculine infertility%Rat
目的 研究弓形虫感染对雄性大鼠生育力的影响,调查男性不育患者弓形虫感染水平,探讨弓形虫感染对男性生殖健康的影响及其可能的致病机制.方法 以30只成年雄性SD清洁级大鼠为研究对象,随机分为感染组,腹腔注射弓形虫速殖子2×10~5/ml 2 ml,阿奇霉素治疗组,腹腔注射弓形虫速殖子2×10~5/ml 2 ml.感染第2天以200mg/(kg·d)剂量喂服阿奇霉素,连喂7 d;正常对照组腹腔注射灭菌生理盐水2 ml.9周后测定各组大鼠的生育力、附睾尾精子数、血清睾酮(T)、黄体生成素(LH)及卵泡刺激素(FSH)水平,睾丸组织酸性磷酸酶(ACP)、碱性磷酸酶(ALP)、乳酸脱氢酶X(LDH-X)和脂质过氧化物酶(LPO)活性.对169例男性不育患者和35例正常生育男性进行弓形虫感染的血清学调查,并检测精浆ACP、α-葡萄糖苷酶(α-Glu)、果糖(Fru)及NO水平.结果 3组动物与雌鼠交配后,雌鼠的受孕率、平均产仔数感染组显著低于正常对照组、阿奇霉素治疗组(P均<0.05).但胎鼠的平均身长、体重、尾长差异则均无统计学意义(P均>0.05);附睾尾精子数感染组(279.4±81.7)×10~6/g与正常对照组(380.9±121.8)×10~6/g、阿奇霉素治疗组(361.2±51.9)×10~6/g比较,差异有统计学意义(P均<0.05);精子活率感染组(48.s4±4.63)%与正常对照组(79.84±2.93)%、阿奇霉素治疗组(69.40±3.57)%比较,差异有统计学意义(P均<0.05);感染组大鼠T浓度与正常对照组、阿奇霉素治疗组相比显著性下降(P均<0.05),血清LH也有下降,FSH无明显变化;感染组ACP为(61.4±7.08)U/mg,显著低于正常对照组(79.2±17.7)U/mg和阿奇霉素治疗组(79.0±13.1)U/mg(P均<O.05);感染组LDH-X为(76.7±7.89)U/mg,显著低于正常对照组(90.3±8.17)U/mg、阿奇霉素治疗组(89.3±13.08)U/mg(P均<0.05).睾丸组织的ALP、LPO酶活性差异均无统计学意义(P均>0.05).169例男性不育者精浆弓形虫感染率为18.35%,显著高于正常生育男性精浆弓形虫感染率的2.86%.31例男性不育患者精浆抗弓形虫IgG抗体阳性组的精浆ACP较阴性组及正常生育组低,差异有统计学意义(P均<0.05);169例男性不育患者精浆NO为(146.68±38.87)μmol/L,显著高于35例正常生育男性的(84.92±26.72)μmol/L(P<0.01);而3组α-Glu、Fru检测结果差异均无统计学意义(P均>0.05).结论 弓形虫感染对雄性生殖健康具有一定程度的影响.
目的 研究弓形蟲感染對雄性大鼠生育力的影響,調查男性不育患者弓形蟲感染水平,探討弓形蟲感染對男性生殖健康的影響及其可能的緻病機製.方法 以30隻成年雄性SD清潔級大鼠為研究對象,隨機分為感染組,腹腔註射弓形蟲速殖子2×10~5/ml 2 ml,阿奇黴素治療組,腹腔註射弓形蟲速殖子2×10~5/ml 2 ml.感染第2天以200mg/(kg·d)劑量餵服阿奇黴素,連餵7 d;正常對照組腹腔註射滅菌生理鹽水2 ml.9週後測定各組大鼠的生育力、附睪尾精子數、血清睪酮(T)、黃體生成素(LH)及卵泡刺激素(FSH)水平,睪汍組織痠性燐痠酶(ACP)、堿性燐痠酶(ALP)、乳痠脫氫酶X(LDH-X)和脂質過氧化物酶(LPO)活性.對169例男性不育患者和35例正常生育男性進行弓形蟲感染的血清學調查,併檢測精漿ACP、α-葡萄糖苷酶(α-Glu)、果糖(Fru)及NO水平.結果 3組動物與雌鼠交配後,雌鼠的受孕率、平均產仔數感染組顯著低于正常對照組、阿奇黴素治療組(P均<0.05).但胎鼠的平均身長、體重、尾長差異則均無統計學意義(P均>0.05);附睪尾精子數感染組(279.4±81.7)×10~6/g與正常對照組(380.9±121.8)×10~6/g、阿奇黴素治療組(361.2±51.9)×10~6/g比較,差異有統計學意義(P均<0.05);精子活率感染組(48.s4±4.63)%與正常對照組(79.84±2.93)%、阿奇黴素治療組(69.40±3.57)%比較,差異有統計學意義(P均<0.05);感染組大鼠T濃度與正常對照組、阿奇黴素治療組相比顯著性下降(P均<0.05),血清LH也有下降,FSH無明顯變化;感染組ACP為(61.4±7.08)U/mg,顯著低于正常對照組(79.2±17.7)U/mg和阿奇黴素治療組(79.0±13.1)U/mg(P均<O.05);感染組LDH-X為(76.7±7.89)U/mg,顯著低于正常對照組(90.3±8.17)U/mg、阿奇黴素治療組(89.3±13.08)U/mg(P均<0.05).睪汍組織的ALP、LPO酶活性差異均無統計學意義(P均>0.05).169例男性不育者精漿弓形蟲感染率為18.35%,顯著高于正常生育男性精漿弓形蟲感染率的2.86%.31例男性不育患者精漿抗弓形蟲IgG抗體暘性組的精漿ACP較陰性組及正常生育組低,差異有統計學意義(P均<0.05);169例男性不育患者精漿NO為(146.68±38.87)μmol/L,顯著高于35例正常生育男性的(84.92±26.72)μmol/L(P<0.01);而3組α-Glu、Fru檢測結果差異均無統計學意義(P均>0.05).結論 弓形蟲感染對雄性生殖健康具有一定程度的影響.
목적 연구궁형충감염대웅성대서생육력적영향,조사남성불육환자궁형충감염수평,탐토궁형충감염대남성생식건강적영향급기가능적치병궤제.방법 이30지성년웅성SD청길급대서위연구대상,수궤분위감염조,복강주사궁형충속식자2×10~5/ml 2 ml,아기매소치료조,복강주사궁형충속식자2×10~5/ml 2 ml.감염제2천이200mg/(kg·d)제량위복아기매소,련위7 d;정상대조조복강주사멸균생리염수2 ml.9주후측정각조대서적생육력、부고미정자수、혈청고동(T)、황체생성소(LH)급란포자격소(FSH)수평,고환조직산성린산매(ACP)、감성린산매(ALP)、유산탈경매X(LDH-X)화지질과양화물매(LPO)활성.대169례남성불육환자화35례정상생육남성진행궁형충감염적혈청학조사,병검측정장ACP、α-포도당감매(α-Glu)、과당(Fru)급NO수평.결과 3조동물여자서교배후,자서적수잉솔、평균산자수감염조현저저우정상대조조、아기매소치료조(P균<0.05).단태서적평균신장、체중、미장차이칙균무통계학의의(P균>0.05);부고미정자수감염조(279.4±81.7)×10~6/g여정상대조조(380.9±121.8)×10~6/g、아기매소치료조(361.2±51.9)×10~6/g비교,차이유통계학의의(P균<0.05);정자활솔감염조(48.s4±4.63)%여정상대조조(79.84±2.93)%、아기매소치료조(69.40±3.57)%비교,차이유통계학의의(P균<0.05);감염조대서T농도여정상대조조、아기매소치료조상비현저성하강(P균<0.05),혈청LH야유하강,FSH무명현변화;감염조ACP위(61.4±7.08)U/mg,현저저우정상대조조(79.2±17.7)U/mg화아기매소치료조(79.0±13.1)U/mg(P균<O.05);감염조LDH-X위(76.7±7.89)U/mg,현저저우정상대조조(90.3±8.17)U/mg、아기매소치료조(89.3±13.08)U/mg(P균<0.05).고환조직적ALP、LPO매활성차이균무통계학의의(P균>0.05).169례남성불육자정장궁형충감염솔위18.35%,현저고우정상생육남성정장궁형충감염솔적2.86%.31례남성불육환자정장항궁형충IgG항체양성조적정장ACP교음성조급정상생육조저,차이유통계학의의(P균<0.05);169례남성불육환자정장NO위(146.68±38.87)μmol/L,현저고우35례정상생육남성적(84.92±26.72)μmol/L(P<0.01);이3조α-Glu、Fru검측결과차이균무통계학의의(P균>0.05).결론 궁형충감염대웅성생식건강구유일정정도적영향.
Objective To study the male reproductive ability of male rats with Toxoplasma gondii ( Tg) infection and investigate the variation of Toxoplasma infection in seminal plasma of infertile patients and explore its mechanism. Methods Thirty SD rats were randomly divided into 3 groups. The rats in the Toxoplasma infection group were administrated intraperitoneally with tachyzoites of Tg. in a dosage of 2 × 10~ 5/ml(2ml) , the rats in the treated group were administered with the same dosage of the tachyzoites and from the second day after the infection they were treated with 200 mg/kg azithromycin for 7 days, and the normal group was given physiological saline. Nine weeks after the infection, the serum sex hormone level, number,vitality, activity and quantity of spermatozoa and activities of enzymes in testa's of the testicular tissues were determined in the male rats. The female rats infected with Tg were matched with normal female rats at a ratio of 1: 2 for one week, and on the 21st day of pregnancy, the number of corpora luteum, sex ratio and the weight, body length and tail length of fetus were measured. The ELISA method was used todetermine the seminal plasma's anti-Tg IgG antibody of the 169 patients with infertility and 35 males with normal fertility. Meanwhile the NO levels in their semina were determined by means of nitric acid reducase. Results The number, activity .vitality, serum level of sex hormones were all lower in the infected rats than those in the normal and treated groups. The number of fetus in the pregnant rats matched with the infected male rats was significantly fewer, but the average body weight, body length, tail length of the fetuses and sex proportion showed no significant difference in comparison with those of the control group. The anti-Toxoplasma gondii antibody positive rate in the masculine infertility patients was 18.35% , being significantly higher than 2. 86% in the normal fertility group(P < 0.05 ). The mean NO level in the semina from the infertility group was (146.68 ± 38. 87) μnol/L , which was significantly higher than (84.92 ± 26.72) μnol/L( P < 0.01) in the fertility group. Conclusion Toxoplasma gondii infection can cause certain influences on the male reproductive ability.
