CAJ | 학술논문

以矮牡丹子叶作为外植体,研究了不同培养基和植物生长调节剂对其再生体系的影响.结果表明:1/2MS培养基有利于子叶不定芽的分化;在附加6-BA 3.0mg·L~(-1)和NAA 0.3mg·L~(-1)的1/2MS培养基上,每个外植体不定芽的分化率达5.22个;将再生芽转接到含有IBA 1.5mg·L~(-1)和NAA 0.5mg·L~(-1)的1/2MS生根培养基上,生根率达54.17%,生根数达3.12条.
이왜모단자협작위외식체,연구료불동배양기화식물생장조절제대기재생체계적영향.결과표명:1/2MS배양기유리우자협불정아적분화;재부가6-BA 3.0mg·L~(-1)화NAA 0.3mg·L~(-1)적1/2MS배양기상,매개외식체불정아적분화솔체5.22개;장재생아전접도함유IBA 1.5mg·L~(-1)화NAA 0.5mg·L~(-1)적1/2MS생근배양기상,생근솔체54.17%,생근수체3.12조.
An experiment was conducted to study the effects of culture medium and plant growth regulators on shoot regeneration from cotyledon of dwarf peony (Paeonia suffruticosa Andr. var. spontanea Rehd.). Results show that 1/2MS medium is suitable for differentiation of adventitious buds. Shoot number reached 5.22 individuals per explant on MS medium supplemented with 3.0mg·L~(-1) 6-BA and 0.3mg·L~(-1) NAA. The rooting rate and root number reached 54.17% and 3.12 after transferred to 1/2MS medium with 1.5mg·L~(-1) IBA and 0.5mg·L~(-1) NAA.