CAJ | 학술논문

为了揭示瘦肉型的长白猪和脂肪型的梅山猪脂肪沉积相关基因的表达差异,采用qRT-PCR方法检测了两猪种背部皮下脂肪组织中硬脂酰辅酶A去饱和酶(SCD)、苹果酸酶(ME1)和解耦联蛋白3(UCP3)基因的表达量在30、60、90、120和150 d的变化.结果表明,两品种SCD的表达模式除90～120 d相反外,其他各日龄之间基本相同;ME1在30～120 d基本相同,而120～150 d呈现出相反的模式;UCP3除120～150 d相同外,其他各日龄之间呈现相反的模式.以上结果初步揭示了两猪种在生长发育过程中SCD、ME1和UCP3表达的发育性变化模式和品种差异,为深入研究脂肪合成代谢的调控机制提供了基础数据.
위료게시수육형적장백저화지방형적매산저지방침적상관기인적표체차이,채용qRT-PCR방법검측료량저충배부피하지방조직중경지선보매A거포화매(SCD)、평과산매(ME1)화해우련단백3(UCP3)기인적표체량재30、60、90、120화150 d적변화.결과표명,량품충SCD적표체모식제90～120 d상반외,기타각일령지간기본상동;ME1재30～120 d기본상동,이120～150 d정현출상반적모식;UCP3제120～150 d상동외,기타각일령지간정현상반적모식.이상결과초보게시료량저충재생장발육과정중SCD、ME1화UCP3표체적발육성변화모식화품충차이,위심입연구지방합성대사적조공궤제제공료기출수거.
To investigate the differential expression patterns of genes related to adipose deposition between Landrace (a leaner, Western breed) and Meishan pigs (a fatty, indigenous, Chinese breed). The expression changes of stearoyl-CoA desaturease(SCD), cytoplasmic malic enzyme (ME1)and uncoupling protein 3(UCP3)genes were measured in two pig breeds at five growth stages (30,60,90,120 and 150 days) by using qRT-PCR approach. The results showed that the similar expression pattern of SCD mRNA was observed except from 90 to 120 days between the two pig breeds. Expression change of ME1 mRNA was similar between two pig breeds from 30 to 120 days, but the opposite expression pattern was exhibited during 120 to 150 days. Expression changes of UCP3 mRNA in two pig breeds were opposite except from 120 to 150 days. These results suggested that the mRNA level of SCD, ME1 and UCP3 genes in backfat tissue are different in postnatal developmental stages between Landrace and Meishan pigs, which provided some data for further study on the three genes for elucidating the molecular mechanism of fat deposition.