中华细胞与干细胞杂志(电子版)
中華細胞與榦細胞雜誌(電子版)
중화세포여간세포잡지(전자판)
CHINESE JOURNAL OF CELL AND STEM CELL
2011年
2期
14-19
,共6页
袁晓妮%何军%侯建全%温端改%鲍晓晶%邱桥成%王乾%钟键%李强
袁曉妮%何軍%侯建全%溫耑改%鮑曉晶%邱橋成%王乾%鐘鍵%李彊
원효니%하군%후건전%온단개%포효정%구교성%왕건%종건%리강
组织相容性Ⅰ类%基因多态性%抗体%肾移植%移植物排斥
組織相容性Ⅰ類%基因多態性%抗體%腎移植%移植物排斥
조직상용성Ⅰ류%기인다태성%항체%신이식%이식물배척
Major histocompatibility complex class Ⅰ chain%Polymorphisms%Specific antibody%Kidney transplantation%Graft rejection
目的 研究组织相容性Ⅰ类相关链A位点(MICA)基因多态性和抗MICA特异性抗体在肾移植排斥反应发生中的意义.方法 采用免疫磁珠液相芯片技术对40例肾移植患者在移植术前和移植术后1个月、3个月、6个月、1年和2年动态检测抗MICA抗体的特异性和阳性分值的变化,同时采用SSOP方法分析16对肾移植供受者的MICA基因分型,并把MICA基因具有相同抗原表位的特征性分为G1组和G2组.结果 在40例肾移植中尸体供肾35例,亲缘活体供肾5例.35例肾移植受者均带肾存活良好,其中33例接受动态随访.移植术前预存抗MICA抗体为12例,其中抗MICA抗体10例、抗HLA-Ⅰ类+MICA抗体2例;4例分为MICA-G1组、6例分为MICA-G2组、2例共为MICA-G1和G2组.16对供受者MICA等位基因频率分布:在MICA-G1组中频率M*002最高,M*007次之;MICA-G2组中频率M*008最高,M*009和M*019次之.33例移植术后有4例新生抗MICA抗体,3例新生抗HLA抗体,均为抗供者特异性抗体和抗非供者特异性抗体.移植术前预存抗MICA抗体,在移植术后1~2年的动态随访中其特异性均未改变,而抗体的阳性分值呈现高低的变化.12例移植术前预存抗MICA抗体中的3例和抗体阴性1例移植术后发生急性排斥反应,临床表现为发热,尿量减少,肌酐和肾动脉阻力指数升高.33例动态随访资料显示:无论是MICA基因频率分布特点,还是抗MICA特异性抗体类型与排斥反应的关系,M*002、M*004、M*008、M*019和M*001基因型最常见,但未见MICA*012和MICA*006基因型.结论 移植术前分析供受者的MICA基因多态性,受者的特异性抗体鉴定和特征性分组;移植术后动态监测抗MICA抗体的变化,是肾移植术后预防急性和慢性移植物排斥的重要靶分子.
目的 研究組織相容性Ⅰ類相關鏈A位點(MICA)基因多態性和抗MICA特異性抗體在腎移植排斥反應髮生中的意義.方法 採用免疫磁珠液相芯片技術對40例腎移植患者在移植術前和移植術後1箇月、3箇月、6箇月、1年和2年動態檢測抗MICA抗體的特異性和暘性分值的變化,同時採用SSOP方法分析16對腎移植供受者的MICA基因分型,併把MICA基因具有相同抗原錶位的特徵性分為G1組和G2組.結果 在40例腎移植中尸體供腎35例,親緣活體供腎5例.35例腎移植受者均帶腎存活良好,其中33例接受動態隨訪.移植術前預存抗MICA抗體為12例,其中抗MICA抗體10例、抗HLA-Ⅰ類+MICA抗體2例;4例分為MICA-G1組、6例分為MICA-G2組、2例共為MICA-G1和G2組.16對供受者MICA等位基因頻率分佈:在MICA-G1組中頻率M*002最高,M*007次之;MICA-G2組中頻率M*008最高,M*009和M*019次之.33例移植術後有4例新生抗MICA抗體,3例新生抗HLA抗體,均為抗供者特異性抗體和抗非供者特異性抗體.移植術前預存抗MICA抗體,在移植術後1~2年的動態隨訪中其特異性均未改變,而抗體的暘性分值呈現高低的變化.12例移植術前預存抗MICA抗體中的3例和抗體陰性1例移植術後髮生急性排斥反應,臨床錶現為髮熱,尿量減少,肌酐和腎動脈阻力指數升高.33例動態隨訪資料顯示:無論是MICA基因頻率分佈特點,還是抗MICA特異性抗體類型與排斥反應的關繫,M*002、M*004、M*008、M*019和M*001基因型最常見,但未見MICA*012和MICA*006基因型.結論 移植術前分析供受者的MICA基因多態性,受者的特異性抗體鑒定和特徵性分組;移植術後動態鑑測抗MICA抗體的變化,是腎移植術後預防急性和慢性移植物排斥的重要靶分子.
목적 연구조직상용성Ⅰ류상관련A위점(MICA)기인다태성화항MICA특이성항체재신이식배척반응발생중적의의.방법 채용면역자주액상심편기술대40례신이식환자재이식술전화이식술후1개월、3개월、6개월、1년화2년동태검측항MICA항체적특이성화양성분치적변화,동시채용SSOP방법분석16대신이식공수자적MICA기인분형,병파MICA기인구유상동항원표위적특정성분위G1조화G2조.결과 재40례신이식중시체공신35례,친연활체공신5례.35례신이식수자균대신존활량호,기중33례접수동태수방.이식술전예존항MICA항체위12례,기중항MICA항체10례、항HLA-Ⅰ류+MICA항체2례;4례분위MICA-G1조、6례분위MICA-G2조、2례공위MICA-G1화G2조.16대공수자MICA등위기인빈솔분포:재MICA-G1조중빈솔M*002최고,M*007차지;MICA-G2조중빈솔M*008최고,M*009화M*019차지.33례이식술후유4례신생항MICA항체,3례신생항HLA항체,균위항공자특이성항체화항비공자특이성항체.이식술전예존항MICA항체,재이식술후1~2년적동태수방중기특이성균미개변,이항체적양성분치정현고저적변화.12례이식술전예존항MICA항체중적3례화항체음성1례이식술후발생급성배척반응,림상표현위발열,뇨량감소,기항화신동맥조력지수승고.33례동태수방자료현시:무론시MICA기인빈솔분포특점,환시항MICA특이성항체류형여배척반응적관계,M*002、M*004、M*008、M*019화M*001기인형최상견,단미견MICA*012화MICA*006기인형.결론 이식술전분석공수자적MICA기인다태성,수자적특이성항체감정화특정성분조;이식술후동태감측항MICA항체적변화,시신이식술후예방급성화만성이식물배척적중요파분자.
Objective To study the association of MHC class Ⅰ chain related antigens A (MICA) polymorphisms and anti-MICA specific antibodies and renal allograft rejection.Mothods The specific anti-MICA antibodies of 40 patients were monitored using Flow PRATM beads from at transplantation and 1,3,6,12 and 24 months post transplantation respectively.The MICA genotypes of 16 patients and donors were determined by PCR-SSOP.Two groups of public epitopes (MICA-G1 and MICA-G2) were characterized.Results Thirty-five out of the 40 renal allografts were from deceased donors and 5 from related living donors.Renal allograft function was good in 35 recipients.Twelve patients had pre-existing antibodies before transplantation in 40 patients (30.0%):10 patients with anti-MICA antibody and 2 patients with anti-MICA antibody and anti-HLA antibody.Four patients were found to have MICA-G1,6 were found to have MICA-G2,and 2 have both MICA-G1 and MICA-G1.As to allele polymorphism distribution of 16 patients and donors,M*002 had the highest frequency for MICA-G1 and M*008 for MICA-G2.Four patients with anti-MICA antibodies and 3 patients with anti-HLA antibodies developed both donor specific and non-donor specific antibodies post transplantation.Antibody specificity did not change in the patients with anti-MICA specific antibodies before transplantation during the follow-up,but positive rate decreased.Among 12recipients with pre-existing antibodies 3 developed acute rejection.For recipients without preexisting antibodies only one developed acute rejection.Fever,hypourocrinia and increased serum creatinine level and resistent index of kidney artery were most often seen during acute rejection.The frequency of M*002,M*004,M *008,M*019 and M*001 were highest while M*002and M*004 were not found.Conclusion MICA genotyping before-transplant,monitoring for MICA antibodies post-transplant,may be useful to establish target factors for acute rejection and chronic rejection,especially with regard to recipient without antibody in kidney transplantation.