中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2010年
10期
1047-1049
,共3页
血管紧张素Ⅱ1型受体%原发性高血压%基因多态性
血管緊張素Ⅱ1型受體%原髮性高血壓%基因多態性
혈관긴장소Ⅱ1형수체%원발성고혈압%기인다태성
Angiotensin Ⅱ type 1 receptor%Essential hypertension%Gene polymorphism
目的 探讨血管紧张素Ⅱ1型受体(AT1R)基因多态性与原发性高血压(EH)之间的关系.方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对200例汉族EH患者(EH组)和192例正常血压者(对照组)的AT1R基因1166A/C及-810A/T多态性进行检测,测定空腹血糖、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)等生物化学指标,分析各基因型和等位基因频率与EH的关系.结果 1166A/C等位基因和基因型频率在EH组和对照组的分布无统计学差异(P均＞0.05),-810A/T各基因型在EH组和对照组间差异有统计学意义(x2=10.862,P=0.004),-810T等位基因频率在EH组显著增高[22.5%(102/400)与11.5%(44/384),x2=12.745,P=0.000],用Logistic回归模型校正了传统危险因素的影响后,-810AT和TT基因型的携带者患高血压的危险性显著增加(P=0.003,OR值为2.57,95%CI:1.37～4.84).结论 AT1R-810A/T多态性与EH发病相关,-810T等位基因可能是EH发病的风险因子.
目的 探討血管緊張素Ⅱ1型受體(AT1R)基因多態性與原髮性高血壓(EH)之間的關繫.方法 應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)對200例漢族EH患者(EH組)和192例正常血壓者(對照組)的AT1R基因1166A/C及-810A/T多態性進行檢測,測定空腹血糖、甘油三酯(TG)、總膽固醇(TC)、低密度脂蛋白膽固醇(LDL-C)及高密度脂蛋白膽固醇(HDL-C)等生物化學指標,分析各基因型和等位基因頻率與EH的關繫.結果 1166A/C等位基因和基因型頻率在EH組和對照組的分佈無統計學差異(P均＞0.05),-810A/T各基因型在EH組和對照組間差異有統計學意義(x2=10.862,P=0.004),-810T等位基因頻率在EH組顯著增高[22.5%(102/400)與11.5%(44/384),x2=12.745,P=0.000],用Logistic迴歸模型校正瞭傳統危險因素的影響後,-810AT和TT基因型的攜帶者患高血壓的危險性顯著增加(P=0.003,OR值為2.57,95%CI:1.37～4.84).結論 AT1R-810A/T多態性與EH髮病相關,-810T等位基因可能是EH髮病的風險因子.
목적 탐토혈관긴장소Ⅱ1형수체(AT1R)기인다태성여원발성고혈압(EH)지간적관계.방법 응용취합매련반응-한제성편단장도다태성(PCR-RFLP)대200례한족EH환자(EH조)화192례정상혈압자(대조조)적AT1R기인1166A/C급-810A/T다태성진행검측,측정공복혈당、감유삼지(TG)、총담고순(TC)、저밀도지단백담고순(LDL-C)급고밀도지단백담고순(HDL-C)등생물화학지표,분석각기인형화등위기인빈솔여EH적관계.결과 1166A/C등위기인화기인형빈솔재EH조화대조조적분포무통계학차이(P균＞0.05),-810A/T각기인형재EH조화대조조간차이유통계학의의(x2=10.862,P=0.004),-810T등위기인빈솔재EH조현저증고[22.5%(102/400)여11.5%(44/384),x2=12.745,P=0.000],용Logistic회귀모형교정료전통위험인소적영향후,-810AT화TT기인형적휴대자환고혈압적위험성현저증가(P=0.003,OR치위2.57,95%CI:1.37～4.84).결론 AT1R-810A/T다태성여EH발병상관,-810T등위기인가능시EH발병적풍험인자.
Objective To investigate whether angiotensin Ⅱ type 1 receptor(AT1R)gene polymorphism is associated with essential hypertension(EH). Methods A total of 200 hypertension patients and 192 normotensive controls were enrolled. The AT1R gene 1166A/C and -810A/T polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-PFLP), and the association between the SNPs and the EH were analyzed statistically. Some biochemical index such as serum glucose (GLU) and total cholesterol (TC),triglyceride (TG), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) were also measured. Results There was no significant difference between two groups of 1166A/C polymorphisms of AT1R gene(P ＞ 0.05 ). However, for the -810A/T polymorphism of AT1R gene, -810 AT and TT genotypes frequencies were significantly higher in EH patients than control (P = 0. 004). The -810T allele frequencies were higher in case than in control (22.5% vs. 11.5% ;P =0.000). We also found an association between EH and -810AT and TT genotypes by logistic regression analysis ( P = 0. 003 ), adjusted for other risk factors. The odds ratio was 2.57 (95% CI:1. 37 ～4. 84). Conclusions AT1R -810A/T polymorphism is associated with EH and -810T allele may be a risk factor of hypertension
