CAJ | 학술논문

目的研究VEGF-C对胰腺癌细胞凋亡的影响.方法建立人胰腺癌细胞株PANC-1裸鼠原位种植瘤模型,原代培养原发和淋巴结转移灶中胰腺癌细胞,通过VEGF-C反义寡核苷酸体外转染抑制其表达,应用RT-PCR及流式细胞术等方法研究对淋巴结转移胰腺癌细胞凋亡及bcl-2的影响.结果体外转染后,原代培养原发和淋巴结转移灶中PANC-1胰腺癌细胞VEGF-C的mRNA表达水平显著降低(P＜0.01).体外转染VEGF-C反义寡核苷酸抑制其表达后,对照组、SODN组、ASODN组淋巴结转移胰腺癌细胞的凋亡率为(2.83±1.01)%、(4.98±2.05)%、(13.22±2.17)%,ASODN组细胞凋亡率显著提高(P＜0.01),而原发灶胰腺癌细胞的凋亡率分别为(3.51±1.38)%、(4.79±2.16)%、(5.33 ±2.18)%,凋亡率无明显影响(P＞0.05);淋巴结转移胰腺癌细胞的反义组bcl-2表达水平明显下调(P＜0.05),而原发灶胰腺癌细胞bcl-2表达水平无明显变化(P＞0.05).结论抑制淋巴结转移灶中胰腺癌细胞VEGF-C的高表达可促进胰腺癌细胞凋亡,这和bcl-2表达下调有关;但对原发灶胰腺癌细胞无明显影响.
목적 연구VEGF-C대이선암세포조망적영향.방법 건립인이선암세포주PANC-1라서원위충식류모형,원대배양원발화림파결전이조중이선암세포,통과VEGF-C반의과핵감산체외전염억제기표체,응용RT-PCR급류식세포술등방법연구대림파결전이이선암세포조망급bcl-2적영향.결과 체외전염후,원대배양원발화림파결전이조중PANC-1이선암세포VEGF-C적mRNA표체수평현저강저(P＜0.01).체외전염VEGF-C반의과핵감산억제기표체후,대조조、SODN조、ASODN조림파결전이이선암세포적조망솔위(2.83±1.01)%、(4.98±2.05)%、(13.22±2.17)%,ASODN조세포조망솔현저제고(P＜0.01),이원발조이선암세포적조망솔분별위(3.51±1.38)%、(4.79±2.16)%、(5.33 ±2.18)%,조망솔무명현영향(P＞0.05);림파결전이이선암세포적반의조bcl-2표체수평명현하조(P＜0.05),이원발조이선암세포bcl-2표체수평무명현변화(P＞0.05).결론 억제림파결전이조중이선암세포VEGF-C적고표체가촉진이선암세포조망,저화bcl-2표체하조유관;단대원발조이선암세포무명현영향.
Objective To investigate the effect of vascular endothelial growth factor C (VEGF-C) on apoptosis of pancreatic cancer cell. Methods Human pancreatic cancer cell line PANC-1 orthotopic implantation tumor model was established in nude mice. Primary pancreatic cancer cells and that derived from lymphatic metastasis were primarily cultured. Expression of VEGF-C was inhibited through antisense oligodeoxynucleotide in vitro transfection. Reverse transcription polynlerase chain reaction (RT-PCR) and flow cytometer were used to detect the effect of VEGF-C on apoptosis of pancreatic cancer cells and bcl-2. Results After in vitro transfection, mRNA expression level of VEGF-C in PANC-1 pancreatic cancer cells significantly decreased (P ＜0. 01 ). Apoptosis rate of pancreatic cancer cells derived from spontaneous lymphatic metastasis was (2. 83 ± 1.01 ) %, ( 4. 98 ± 2. 05 ) %,and ( 13.22 ±2. 17) % respectively for control group, SODN group and ASODN group after in vitro transfection among which apoptosis rate in ASODN group increased significantly (P ＜0. 01 ). However, apoptosis rate for pancreatic cancer cells derived from primary tumor had no obvious change (P ＞0.05), with (3.51 ±1.38)%, (4.76 ±2. 16 ) %, and (5. 33 ± 2. 18 ) % respectively in control group, SODN group and ASODN group. The expression level of bcl-2 in pancreatic cancer cells derived from spontaneous lymphatic metastasis decreased significantly (P ＜0. 05) while it had no obvious change in primary pancreatic cancer cells (P ＞ 0. 05). Conclusion To inhibit expression of VEGF-C in pancreatic cancer cell can promote apoptosis of pancreatic cancer cell, which is relevant to downregulation of bcl-2;however, it has no obvious effect on primary pancreatic cancer.