国际输血及血液学杂志
國際輸血及血液學雜誌
국제수혈급혈액학잡지
INTERNATIONAL JOURNAL OF BLOOD TRANSFUSION AND HEMATOLOGY
2010年
6期
492-495
,共4页
喻琼%苏宇清%梁延连%李大成%曾健强%邓志辉
喻瓊%囌宇清%樑延連%李大成%曾健彊%鄧誌輝
유경%소우청%량연련%리대성%증건강%산지휘
ABO血型%天然抗体%ABO基因
ABO血型%天然抗體%ABO基因
ABO혈형%천연항체%ABO기인
ABO blood group%natural antibody%ABO
目的 从ABO基因水平探讨正常人群血型鉴定中出现天然抗体缺失的机制.方法 部分或全部缺失抗体导致ABO血型正反定型不一致的15个正常健康个体运用检验红细胞表面血型抗原最为敏感的方法-吸收放散试验未能检测出其抗原性.PCR扩增后直接序列分析ABO基因全长编码区及5'端调控(CBF/NF-Y)增强子区域多态性.结果 在常规的血清学反向定型实验中,这15个标本的血清中部分或全部缺失抗体,吸收放散实验与A或B型红细胞反应均为阴性.12个标本的ABO基因分析定型、增强子区域多态性与血清学结果相符合,1个O表型的标本中存在B101等位基因,2个B表型标本中检测出A102等位基因.结论 天然ABO抗体缺失的分子机制可能部分被揭示:ABO基因编码少量表达抗原,按照天然抗体产生规律,导致不能产生相应的ABO抗体.ABO基因分型在正反定型不符的疑难血液标本鉴定中,是血清学方法 非常有益的补充手段,是正确鉴定血型的有效方法.
目的 從ABO基因水平探討正常人群血型鑒定中齣現天然抗體缺失的機製.方法 部分或全部缺失抗體導緻ABO血型正反定型不一緻的15箇正常健康箇體運用檢驗紅細胞錶麵血型抗原最為敏感的方法-吸收放散試驗未能檢測齣其抗原性.PCR擴增後直接序列分析ABO基因全長編碼區及5'耑調控(CBF/NF-Y)增彊子區域多態性.結果 在常規的血清學反嚮定型實驗中,這15箇標本的血清中部分或全部缺失抗體,吸收放散實驗與A或B型紅細胞反應均為陰性.12箇標本的ABO基因分析定型、增彊子區域多態性與血清學結果相符閤,1箇O錶型的標本中存在B101等位基因,2箇B錶型標本中檢測齣A102等位基因.結論 天然ABO抗體缺失的分子機製可能部分被揭示:ABO基因編碼少量錶達抗原,按照天然抗體產生規律,導緻不能產生相應的ABO抗體.ABO基因分型在正反定型不符的疑難血液標本鑒定中,是血清學方法 非常有益的補充手段,是正確鑒定血型的有效方法.
목적 종ABO기인수평탐토정상인군혈형감정중출현천연항체결실적궤제.방법 부분혹전부결실항체도치ABO혈형정반정형불일치적15개정상건강개체운용검험홍세포표면혈형항원최위민감적방법-흡수방산시험미능검측출기항원성.PCR확증후직접서렬분석ABO기인전장편마구급5'단조공(CBF/NF-Y)증강자구역다태성.결과 재상규적혈청학반향정형실험중,저15개표본적혈청중부분혹전부결실항체,흡수방산실험여A혹B형홍세포반응균위음성.12개표본적ABO기인분석정형、증강자구역다태성여혈청학결과상부합,1개O표형적표본중존재B101등위기인,2개B표형표본중검측출A102등위기인.결론 천연ABO항체결실적분자궤제가능부분피게시:ABO기인편마소량표체항원,안조천연항체산생규률,도치불능산생상응적ABO항체.ABO기인분형재정반정형불부적의난혈액표본감정중,시혈청학방법 비상유익적보충수단,시정학감정혈형적유효방법.
Objective To investigate the mechanism of natural ABO antibodies deficiency in ABO blood group of common population. Methods Fifteen common healthy individuals with ABO inconsistency in forward and reverse typing caused by partly or entirely lacking antibodies were accessed by adsorptionelution test. DNA analysis on whole code sequences of ABO gene and the polymorphisms of CBF/NF-Y enhancer region were preformed in these individuals. Results In routine ABO grouping, 15 blood samples were shown to be entirely or partly deficient of natural antibodies. Adsorption-elution tests performed by testing A or B RBC were all negative. Sequences of ABC code gene and polymorphisms of the enhance region from 12 samples were consistent with the ABO forward type. Interesting, one sample typed O phenotype was characterized as heterozygous B101/O02, and A102 allele was demonstrable in two cases with B phenotype. Conclusion The molecular mechanisms in deficiency of natural ABO antibody could be clarified partly. ABH antigens are expressed little on red cell membranes, so that relevant antibodies are not formed.ABO genotyping technique is a good method for blood bank service and a complement method for determination of donor blood status by seroloty.
