中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2012年
5期
521-524
,共4页
朱兵清%徐丽%周海健%邵祝军
硃兵清%徐麗%週海健%邵祝軍
주병청%서려%주해건%소축군
C群脑膜炎奈瑟菌%血清杀菌试验%酶联免疫吸附试验
C群腦膜炎奈瑟菌%血清殺菌試驗%酶聯免疫吸附試驗
C군뇌막염내슬균%혈청살균시험%매련면역흡부시험
Neisseria meningitidis group C%Serum bactericidal assay%Enzyme-linked immunosorbent assay
目的 比较血清杀菌试验(SBA)和酶联免疫吸附试验(ELISA)测定两种C群脑膜炎奈瑟菌(Nm)疫苗免疫后血清抗体滴度的差异.方法 采用SBA测定75名未免疫健康成年人(40～70岁)血清、143名3～8月龄婴儿及194名3～5岁儿童NmA和C群结合疫苗或A+C群多糖疫苗免疫前后血清中Nm杀菌抗体水平,然后用ELISA测定相应血清的Nm特异性IgG含量,并利用线性相关与回归分析两种测定结果的相关性.结果 未免疫健康成年人血清中Nm杀菌抗体水平和特异性IgG含量之间的相关性较高(r=0.814 33,P＜0.001)；3～8月龄婴儿和3～5岁儿童接种结合/多糖疫苗前,Nm杀菌抗体水平和特异性IgG含量之间相关性较差(3～8月龄:r=0.140 64,P＞0.l00/r=0.2899,P＜0.05;3 ～5岁:r=0.540 40,P＜0.05/r=0194 36,P＜0.05),接种1剂结合疫苗后,杀菌抗体水平和特异性IgG含量之间的相关性较好(r=0.809 38,P＜0.001和r=0.837 23,P＜0.001)；接种多糖疫苗后,杀菌抗体水平和特异性IgG含量之间的相关性较差(r＜0.500 00).结论 健康成年人血清和结合疫苗免疫后的婴幼儿血清中特异性C群Nm IgG含量可以间接反映血清杀菌抗体水平,ELISA方法可以替代SBA.但ELISA检测方法不适用于3～8月龄婴儿多糖疫苗免疫后的效果评价.
目的 比較血清殺菌試驗(SBA)和酶聯免疫吸附試驗(ELISA)測定兩種C群腦膜炎奈瑟菌(Nm)疫苗免疫後血清抗體滴度的差異.方法 採用SBA測定75名未免疫健康成年人(40～70歲)血清、143名3～8月齡嬰兒及194名3～5歲兒童NmA和C群結閤疫苗或A+C群多糖疫苗免疫前後血清中Nm殺菌抗體水平,然後用ELISA測定相應血清的Nm特異性IgG含量,併利用線性相關與迴歸分析兩種測定結果的相關性.結果 未免疫健康成年人血清中Nm殺菌抗體水平和特異性IgG含量之間的相關性較高(r=0.814 33,P＜0.001)；3～8月齡嬰兒和3～5歲兒童接種結閤/多糖疫苗前,Nm殺菌抗體水平和特異性IgG含量之間相關性較差(3～8月齡:r=0.140 64,P＞0.l00/r=0.2899,P＜0.05;3 ～5歲:r=0.540 40,P＜0.05/r=0194 36,P＜0.05),接種1劑結閤疫苗後,殺菌抗體水平和特異性IgG含量之間的相關性較好(r=0.809 38,P＜0.001和r=0.837 23,P＜0.001)；接種多糖疫苗後,殺菌抗體水平和特異性IgG含量之間的相關性較差(r＜0.500 00).結論 健康成年人血清和結閤疫苗免疫後的嬰幼兒血清中特異性C群Nm IgG含量可以間接反映血清殺菌抗體水平,ELISA方法可以替代SBA.但ELISA檢測方法不適用于3～8月齡嬰兒多糖疫苗免疫後的效果評價.
목적 비교혈청살균시험(SBA)화매련면역흡부시험(ELISA)측정량충C군뇌막염내슬균(Nm)역묘면역후혈청항체적도적차이.방법 채용SBA측정75명미면역건강성년인(40～70세)혈청、143명3～8월령영인급194명3～5세인동NmA화C군결합역묘혹A+C군다당역묘면역전후혈청중Nm살균항체수평,연후용ELISA측정상응혈청적Nm특이성IgG함량,병이용선성상관여회귀분석량충측정결과적상관성.결과 미면역건강성년인혈청중Nm살균항체수평화특이성IgG함량지간적상관성교고(r=0.814 33,P＜0.001)；3～8월령영인화3～5세인동접충결합/다당역묘전,Nm살균항체수평화특이성IgG함량지간상관성교차(3～8월령:r=0.140 64,P＞0.l00/r=0.2899,P＜0.05;3 ～5세:r=0.540 40,P＜0.05/r=0194 36,P＜0.05),접충1제결합역묘후,살균항체수평화특이성IgG함량지간적상관성교호(r=0.809 38,P＜0.001화r=0.837 23,P＜0.001)；접충다당역묘후,살균항체수평화특이성IgG함량지간적상관성교차(r＜0.500 00).결론 건강성년인혈청화결합역묘면역후적영유인혈청중특이성C군Nm IgG함량가이간접반영혈청살균항체수평,ELISA방법가이체대SBA.단ELISA검측방법불괄용우3～8월령영인다당역묘면역후적효과평개.
Objective To analyze the levels of human serum antibody against Neisseria meningitidis serogroup C measured by serum bactericidal assay (SBA) and ELISA.Methods SBA and a modified ELISA were applied to measure the serum bactericidal titer and the specific concentration of immunoglobulin G (IgG) against meningoeoccal serogroup C in sera samples.Seventy-five sera were from healthy adults without undertaking vaccination while another 429 and 388pre- and post- vaccinated sera were from 143 infants and 194 young children immunized with conjugate vaccine or polysaccharide vaccine,respectively.Correlation between serum bactericidal titer and the concentration of specific IgG against meningococcal serogroup C was analyzed.Results The concentration of meningococcal serogroup C specific IgG in healthy adults showed a strong correlation (r=0.814 33,P＜0.001 ) with serum bactericidal titer through linear regression analysis.Weak correlation was observed between SBA titers and IgG concentration in pre vaccinated sera of infants and children ( conjugate/polysaccharide vaccine ) ( infants:r =0.140 64,P ＞ 0.100/r =0.2899,P＜0.05; children:r=0.540 40,P＜0.05/r=0.194 36,P＜0.05).After immunization with 2-dose conjugate vaccine in infants and 1-dose in children,a strong correlation between the two panels of results was observed (r=0.809 38,P＜0.001 and r=0.837 23,P＜0.001 respectively).However after immunization with polysaccharide vaccine,the correlation between serum bactericidal titer and concentration of specific IgG was weak (r＜0.500 00).Conclusion Among healthy adults and post vaccinated infants or young children immunized with conjugate vaccine,the concentration of specific IgG was comparable to the serum bactericidal titer against meningococcal serogroup C.However,it was not unfavorable to use ELISA as the principal means of measuring serum antibody responses to polysaccharide vaccine for infants under 1 year old.
