CAJ | 학술논문

目的探讨川芎嗪预先给药对胎鼠海马神经细胞缺氧/复氧时c-fos和热休克蛋白70(HSP70)表达的影响.方法胎鼠海马神经细胞培养鉴定后,随机分为5组(n=24):正常对照组(C组)、缺氧/复氧损伤组(A/R组)、不同浓度川芎嗪预先给药组(L组、M组和H组).C组不制备缺氧/复氧模型;A/R组、L组、M组和H组制备缺氧/复氧模型;L组、M组和H组加入川芎嗪,终浓度分别为60、200和800μg/ml,孵育1 h后制备缺氧/复氧模型.缺氧/复氧模型制备方法:海马神经细胞置入90%N2-10%CO2培养箱中孵育2 h诱导缺氧,然后放入37 ℃、5%CO2培养箱中复氧24 h.处理结束后测定海马神经细胞凋率、细胞活力、c-fos和HSP70的表达水平.结果与C组比较,A/R组、L组和H组海马神经细胞活力降低,细胞凋亡率升高(P＜0.01);与A/R组比较,L组、M组和H组海马神经细胞活力升高,细胞凋亡率降低,c-fos表达下调,HSP70表达上调(P＜0.05);与L组比较,M组和H组海马神经细胞活力升高,细胞凋亡率降低,c-fos表达下调,HSP70表达上调(P＜0.05);与M组比较,H组细胞活力下降,细胞凋亡率升高,c-fos表达上调,HSP70表达下调(P＜0.01).结论川芎嗪预先给药抑制胎鼠海马神经细胞缺氧/复氧时细胞凋亡的机制可能与下调c-fos表达,上调HSP70表达有关.
목적 탐토천궁진예선급약대태서해마신경세포결양/복양시c-fos화열휴극단백70(HSP70)표체적영향.방법 태서해마신경세포배양감정후,수궤분위5조(n=24):정상대조조(C조)、결양/복양손상조(A/R조)、불동농도천궁진예선급약조(L조、M조화H조).C조불제비결양/복양모형;A/R조、L조、M조화H조제비결양/복양모형;L조、M조화H조가입천궁진,종농도분별위60、200화800μg/ml,부육1 h후제비결양/복양모형.결양/복양모형제비방법:해마신경세포치입90%N2-10%CO2배양상중부육2 h유도결양,연후방입37 ℃、5%CO2배양상중복양24 h.처리결속후측정해마신경세포조솔、세포활력、c-fos화HSP70적표체수평.결과 여C조비교,A/R조、L조화H조해마신경세포활력강저,세포조망솔승고(P＜0.01);여A/R조비교,L조、M조화H조해마신경세포활력승고,세포조망솔강저,c-fos표체하조,HSP70표체상조(P＜0.05);여L조비교,M조화H조해마신경세포활력승고,세포조망솔강저,c-fos표체하조,HSP70표체상조(P＜0.05);여M조비교,H조세포활력하강,세포조망솔승고,c-fos표체상조,HSP70표체하조(P＜0.01).결론천궁진예선급약억제태서해마신경세포결양/복양시세포조망적궤제가능여하조c-fos표체,상조HSP70표체유관.
Objective To investigate the effect of tetramethylpyrazine pretreatment on the expression of cfos and heat shock protein (HSP70) during hypoxia-reoxygenation (H/R) in cultured fetal rat hippocampal neurons. Methods After the neurons were cultured and identified, they were randomly divided into 5 groups ( n = 24each): control group (group C), H/R group, and low, median and high concentration of tetramethylpyrazine pretreatment groups (group L, M and H). The neurons were exposed to 2 h of hypoxia followed by 24 h of reoxygenation. Tetramethylpyrazine was added with the final concentrations of 60, 200 and 800 μg/ml in group L, M and H respectively, and then the neurons were incubated for 1 h before H/R. The apoptosis rate, cell viability and expression of c-fos and HSP70 were detected. Results The cell viability was significantly lower, while the apoptosis rate was significantly higher in group A/R, L and H than in group C (P ＜0.01). The cell viability and HSP70expression were significantly higher, while the apoptosis rate and c-fos expression were significantly lower in group L, M and H than in group A/R, and in group M and H than in group L (P＜ 0.05). The cell viability and HSP70expression were significantly lower, while the apoptosis rate and c-fos expression were significantly higher in group H than in group M ( P ＜ 0.01 ).Conclusion The mechanism by which tetramethylpyrazine pretreatment inhibits the apoptosis in cultured fetal rat hippocampal neurons during H/R may be related to the dowm-regulation of c-fos expression and up-regulation of HSP70 expression.