CAJ | 학술논문

采用柱层析方法从银杏叶内生真菌Chaetomium globosum ZY-22的培养菌丝体提取物中分离得到脑苷脂B(1)、脑苷脂C(2)、尿囊素(3)、9(11)-去氢麦角甾醇过氧化物(4)以及4,6,8,22-四烯-3-酮-麦角甾烷(5)和球毛壳甲素(6)共6个次生代谢物;经波谱分析确定了6个化合物的结构,其中脑苷脂B、脑苷脂C和尿囊素是首次从内生真菌中得到;海虾致死试验结果显示,化合物1～6在10 μg/mL浓度下对丰年虾的致死率分别为1.6%、4.2%、7.4%、16.9%、12.8%、83.6%、表明球毛壳甲素对海虾表现出很强的毒性作用.
채용주층석방법종은행협내생진균Chaetomium globosum ZY-22적배양균사체제취물중분리득도뇌감지B(1)、뇌감지C(2)、뇨낭소(3)、9(11)-거경맥각치순과양화물(4)이급4,6,8,22-사희-3-동-맥각치완(5)화구모각갑소(6)공6개차생대사물;경파보분석학정료6개화합물적결구,기중뇌감지B、뇌감지C화뇨낭소시수차종내생진균중득도;해하치사시험결과현시,화합물1～6재10 μg/mL농도하대봉년하적치사솔분별위1.6%、4.2%、7.4%、16.9%、12.8%、83.6%、표명구모각갑소대해하표현출흔강적독성작용.
Six metabolites cerebroside B (1),cerebroside C (2),allantoin (3),9(11)-dehyoergosterol peroxide (4) and ergosta-4,6,8,22-tetraen-3-one (5),chaetoglobosin A (6) were isolated by column chromatography from the extract of cultural mycelium of fungus Chaetomium globosum ZY-22,an endophyte in the leaves of Ginkgo biloba.Structures of them were established by spectroscopic methods.Among of them,cerebroside B,cerebroside C,allantoin were firstly obtained from endophytic fungus;The result of brine shrimp bioassay showed the mortality rates of them to Artemia salina are 1.6%,4.2%,7.4%,16.9%,12.8% and 83.6% respectively at the concentration of 10 μg/mL,chaetoglobosin A showed significant toxic effect on brine shrimp.