CAJ | 학술논문

从水稻中克隆了一个在稻属植物中高度保守和组成型表达的丝氨酸/苏氨酸蛋白激酶基因(OsSTK).该基因包含两个外显子和一个114 bp的小内含子序列,预测编码一个419个氨基酸的蛋白质.该基因推导的氨基酸序列与其它已知序列的一致性均低于52%.利用从不同种和类型的野生稻克隆的部分该基因序列构建的系统树与野生稻的分类和进化关系相一致.OSPK N-端拥有一段富含丝氨酸、碱性氨基酸和带电荷氨基酸的特异性导肽序列,其中包含"GDGDGDGDG"短重复序列.由于该基因蛋白激酶结构域中的Vib, VⅢ和XI亚结构域中同时具有酪氨酸蛋白激酶和丝氨酸/苏氨酸蛋白激酶的特性,推测该基因可能同时具有催化酪氨酸和丝氨酸、苏氨酸磷酸化的双重功能.
종수도중극륭료일개재도속식물중고도보수화조성형표체적사안산/소안산단백격매기인(OsSTK).해기인포함량개외현자화일개114 bp적소내함자서렬,예측편마일개419개안기산적단백질.해기인추도적안기산서렬여기타이지서렬적일치성균저우52%.이용종불동충화류형적야생도극륭적부분해기인서렬구건적계통수여야생도적분류화진화관계상일치.OSPK N-단옹유일단부함사안산、감성안기산화대전하안기산적특이성도태서렬,기중포함"GDGDGDGDG"단중복서렬.유우해기인단백격매결구역중적Vib, VⅢ화XI아결구역중동시구유락안산단백격매화사안산/소안산단백격매적특성,추측해기인가능동시구유최화락안산화사안산、소안산린산화적쌍중공능.
A cytoplasmic serine/thronine protein kinase gene (OsSTK), had been cloned from Oryza genus. It was found high conservative and constitutive expression in Oryza. OsSTK gene had two exons, separated by 114 bp short intron. The open reading frame of OsSTK gene that predicted encoded a 419 amino acids protein. The amino acid sequence of OsSTK had low identities (less than 53%) with any other known protein kinase. The phylogenetic tree based on the partial DNA sequences of OsSTK from different species and types of wild rice and cultivated rice, was close to the taxation system of rice. Interestingly, OsSTK had a serine, including basic amino acids and charged amino acids abundant polypeptide with a "GDGDGDGDG" sequence at N-terminal that had not been found in any other genes. OsSTK may play dual specificity that phosphorylates both serine/thronine and tyrosone, because the amino acids module of Vib, VⅢ and XI catalytic domain have both the serine/thronine and tyrosine kinase characters.