上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2010年
1期
55-58
,共4页
陈素芸%姜磊%管樑%卢伟京%卢浩%杨维成%李彪%李培勇%杜晓宁%李良君%朱承谟
陳素蕓%薑磊%管樑%盧偉京%盧浩%楊維成%李彪%李培勇%杜曉寧%李良君%硃承謨
진소예%강뢰%관량%로위경%로호%양유성%리표%리배용%두효저%리량군%주승모
呼气试验%小鼠%国产~(13)C-美沙西丁
呼氣試驗%小鼠%國產~(13)C-美沙西丁
호기시험%소서%국산~(13)C-미사서정
breath test%mice%domestically synthesized ~(13)C-methacetin
目的 建立一套简单易操作并在小鼠活体上进行的~(13)C呼气试验检测系统,并探讨国产~(13)C-美沙西丁呼气试验(~(13)C-MBT)对小鼠急性肝损伤的诊断价值.方法 ~(13)C-美沙西丁经扑热息痛甲烷化合成标记.采用四氯化碳(CCl_4)腹腔注射制作小鼠急性肝损伤模型,造模后饲养1个月复制小鼠急性肝损伤恢复模型,行肝脏病理组织学检查和肝功能血液生化学指标检测,于多个时间点采集活体小鼠~(13)C-美沙西丁灌胃后呼出的气体,以红外线能谱仪(IRIS)检测并绘制呼气峰值(DOB)曲线.结果 正常对照组小鼠于灌胃给药后6-8 min时呼气~(13)C达到峰值(51.9±2.04),随后缓慢下降至本底值;模型组于灌胃给药后16 min左右达到峰值(26.37±5.74),随后亦缓慢下降至本底值.两组间差异有统计学意义(P<0.05).当急性肝损伤恢复模型组和对照组小鼠在相同条件下饲养1个月后,重复~(13)C-美沙西丁呼气检测后两组DOB峰值及达峰时间差异无统计学意义(P>0.05).结论 ~(13)C-MBT可在小鼠活体状态下准确、方便地采集和检测呼出气体的~(13)CO_2变化,并正确评估其肝功能的损伤和恢复情况.
目的 建立一套簡單易操作併在小鼠活體上進行的~(13)C呼氣試驗檢測繫統,併探討國產~(13)C-美沙西丁呼氣試驗(~(13)C-MBT)對小鼠急性肝損傷的診斷價值.方法 ~(13)C-美沙西丁經撲熱息痛甲烷化閤成標記.採用四氯化碳(CCl_4)腹腔註射製作小鼠急性肝損傷模型,造模後飼養1箇月複製小鼠急性肝損傷恢複模型,行肝髒病理組織學檢查和肝功能血液生化學指標檢測,于多箇時間點採集活體小鼠~(13)C-美沙西丁灌胃後呼齣的氣體,以紅外線能譜儀(IRIS)檢測併繪製呼氣峰值(DOB)麯線.結果 正常對照組小鼠于灌胃給藥後6-8 min時呼氣~(13)C達到峰值(51.9±2.04),隨後緩慢下降至本底值;模型組于灌胃給藥後16 min左右達到峰值(26.37±5.74),隨後亦緩慢下降至本底值.兩組間差異有統計學意義(P<0.05).噹急性肝損傷恢複模型組和對照組小鼠在相同條件下飼養1箇月後,重複~(13)C-美沙西丁呼氣檢測後兩組DOB峰值及達峰時間差異無統計學意義(P>0.05).結論 ~(13)C-MBT可在小鼠活體狀態下準確、方便地採集和檢測呼齣氣體的~(13)CO_2變化,併正確評估其肝功能的損傷和恢複情況.
목적 건립일투간단역조작병재소서활체상진행적~(13)C호기시험검측계통,병탐토국산~(13)C-미사서정호기시험(~(13)C-MBT)대소서급성간손상적진단개치.방법 ~(13)C-미사서정경복열식통갑완화합성표기.채용사록화탄(CCl_4)복강주사제작소서급성간손상모형,조모후사양1개월복제소서급성간손상회복모형,행간장병리조직학검사화간공능혈액생화학지표검측,우다개시간점채집활체소서~(13)C-미사서정관위후호출적기체,이홍외선능보의(IRIS)검측병회제호기봉치(DOB)곡선.결과 정상대조조소서우관위급약후6-8 min시호기~(13)C체도봉치(51.9±2.04),수후완만하강지본저치;모형조우관위급약후16 min좌우체도봉치(26.37±5.74),수후역완만하강지본저치.량조간차이유통계학의의(P<0.05).당급성간손상회복모형조화대조조소서재상동조건하사양1개월후,중복~(13)C-미사서정호기검측후량조DOB봉치급체봉시간차이무통계학의의(P>0.05).결론 ~(13)C-MBT가재소서활체상태하준학、방편지채집화검측호출기체적~(13)CO_2변화,병정학평고기간공능적손상화회복정황.
Objective To establish a convenient ~(13)C-breath test system in live mice,and investigate the value of ~(13)C-methacetin breath test(~(13)C-MBT) in the diagnosis of acute liver damage of mice with domestically synthesized ~(13)C-methacetin. Methods Domestically synthesized ~(13)C.methacetin was prepared from aeamol by methylation. Abdominal injection of CCl_4 was adopted to duplicate acute liver damage of mice,then the mice were housed under normal laboratory condition for a whole month to gain recovery,which were indentified by hepatic pathological examinations and biochemical teats of liver function.After fasting, the mice were orally administered ~(13)C-methacetin,and the expired air was collected at various time points. Infrared spectrometer was employed, and delm over baseline(DOB) curves of ~(13)C-exhalation were drawn. Results Six to eight min after administration of ~(13)C-methacetin,the rate of ~(13)C-exhalation peaked in control group(51.9±2.04), and decreased thereafter. Sixteen min after administration of ~(13)C-methacetin,the rate of ~(13)JC-exhalation peaked in model group(26.37±5.74), and decreased thereafter.There were significant differences between these two groups(P<0.05).There was no significant difference in peak value and time to reach the peak on DOB curves of ~(13)C-methacetin breath test after the two groups of mice were housed under the same condition for a month(P>0.05).Conclution ~(13)C-MBT facilitates the collection and evaluation of ~(13)CO_2 in the expired air of live mice,and yields precise reflection of alterations of liver function in acute liver injury and functional recovery.
