CAJ | 학술논문

使用超高效液相色谱-电喷雾串联四极杆质谱(UPLC-ESI-MS/MS)在多反应离子监测(MRM)模式下分离鉴定了烟草中7种潜香物质.烟样经甲醇提取,XAD-2柱分离纯化,选择中性丢失162 u扫描确定母离子.通过对母离子二级质谱扫描(MS2),并根据m/z 192.7、179.2、163.1、134.7、133.2等特征碎片离子峰分析结果确定了7种潜香型物质,分别为熊果苷、香豆酸-葡萄糖苷、香豆素奎尼酸、新绿原酸、绿原酸、葡萄糖基阿魏酸、3-氧化紫罗兰醇葡萄糖苷.
사용초고효액상색보-전분무천련사겁간질보(UPLC-ESI-MS/MS)재다반응리자감측(MRM)모식하분리감정료연초중7충잠향물질.연양경갑순제취,XAD-2주분리순화,선택중성주실162 u소묘학정모리자.통과대모리자이급질보소묘(MS2),병근거m/z 192.7、179.2、163.1、134.7、133.2등특정쇄편리자봉분석결과학정료7충잠향형물질,분별위웅과감、향두산-포도당감、향두소규니산、신록원산、록원산、포도당기아위산、3-양화자라란순포도당감.
A method for identification of 7 aroma precursors in tobacco was established by using ultra performance liquid chromatography-tandem mass spectrometry interfaced with electro-spray ionization(UPLC-ESI-MS/MS) in the multiple reaction monitoring(MRM) mode. Aroma precursors in tobacco were extracted with methanol, cleaned up with XAD-2 column, then detected and identified by ESI-MS/MS with the neutral loss method. According to the characteristic product ions of m/z 192.7, 179.2, 163.1, 134.7, 133.2 acquired by product ion scan(MS~2), 7 potential flavor compounds are identified, they are arbutin, trans-p-coumaric acid-4-o-β-D-glucopyrannoside, coumaroylquinic acid, neochlorogenic acid,chlorogenic acid, feruloylglycoside, 3-oxo-α-ionol-β-D-glycoside. The method is rapid, convenient and suitable for the determination of aroma precursors in tobacco leaves.