中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2011年
15期
2022-2024
,共3页
陈健智%李璇%姚岐%曹修淮
陳健智%李璇%姚岐%曹脩淮
진건지%리선%요기%조수회
结直肠肿瘤%E-钙黏蛋白%β-连接素%肿瘤转移
結直腸腫瘤%E-鈣黏蛋白%β-連接素%腫瘤轉移
결직장종류%E-개점단백%β-련접소%종류전이
Colorectal neoplasms%E-cadherin%β-catenin%Neoplasms metastasis
目的 探讨上皮型钙黏附蛋白(E-cadherin)和β-连接素(β-catenin)在结直肠癌及转移灶组织中的表达及临床意义。方法 用免疫组织化学方法检测E-cadherin和β-catenin在20例正常结肠黏膜(A组)、25例无淋巴结转移的结直肠癌(B组)、39例伴淋巴结转移的结直肠癌原发灶和同一个体肠系膜淋巴结转移灶组织(C组)中的表达情况，观察E-cadherin和β-catenin表达与肿瘤转移及预后的相关性。结果 B组和C组E-cadherin表达阳性率均明显低于A组(U=4.681、U=5.775，均P=0.000)；C组原发灶中E-cadherin的表达强阳性率(5.1％)明显低于B组(16.0％) (U=2.149，P=0.032)，C组转移灶与原发灶组织中E-cadherin的表达差异无统计学意义(P=0.186)；β-catenin在B组与C组、C组原发灶与转移灶组织中的表达差异均无统计学意义(P=0.235、P=0.715)；C组E-cadherin胞膜的表达下调和β-catenin胞膜的表达下调显示正相关(r=0.387，P=O.024)，B组E-cadherin胞膜的表达下调和β-catenin胞膜的表达下调无明显相关性(r=0.080,P=0.723)。结论 E-Cadherin表达下调与结直肠癌伴有淋巴结转移有关，且与β-catenin的表达下调有明显的相关性，而β-catenin表达与结直肠癌伴淋巴结转移无关。
目的 探討上皮型鈣黏附蛋白(E-cadherin)和β-連接素(β-catenin)在結直腸癌及轉移竈組織中的錶達及臨床意義。方法 用免疫組織化學方法檢測E-cadherin和β-catenin在20例正常結腸黏膜(A組)、25例無淋巴結轉移的結直腸癌(B組)、39例伴淋巴結轉移的結直腸癌原髮竈和同一箇體腸繫膜淋巴結轉移竈組織(C組)中的錶達情況，觀察E-cadherin和β-catenin錶達與腫瘤轉移及預後的相關性。結果 B組和C組E-cadherin錶達暘性率均明顯低于A組(U=4.681、U=5.775，均P=0.000)；C組原髮竈中E-cadherin的錶達彊暘性率(5.1％)明顯低于B組(16.0％) (U=2.149，P=0.032)，C組轉移竈與原髮竈組織中E-cadherin的錶達差異無統計學意義(P=0.186)；β-catenin在B組與C組、C組原髮竈與轉移竈組織中的錶達差異均無統計學意義(P=0.235、P=0.715)；C組E-cadherin胞膜的錶達下調和β-catenin胞膜的錶達下調顯示正相關(r=0.387，P=O.024)，B組E-cadherin胞膜的錶達下調和β-catenin胞膜的錶達下調無明顯相關性(r=0.080,P=0.723)。結論 E-Cadherin錶達下調與結直腸癌伴有淋巴結轉移有關，且與β-catenin的錶達下調有明顯的相關性，而β-catenin錶達與結直腸癌伴淋巴結轉移無關。
목적 탐토상피형개점부단백(E-cadherin)화β-련접소(β-catenin)재결직장암급전이조조직중적표체급림상의의。방법 용면역조직화학방법검측E-cadherin화β-catenin재20례정상결장점막(A조)、25례무림파결전이적결직장암(B조)、39례반림파결전이적결직장암원발조화동일개체장계막림파결전이조조직(C조)중적표체정황，관찰E-cadherin화β-catenin표체여종류전이급예후적상관성。결과 B조화C조E-cadherin표체양성솔균명현저우A조(U=4.681、U=5.775，균P=0.000)；C조원발조중E-cadherin적표체강양성솔(5.1％)명현저우B조(16.0％) (U=2.149，P=0.032)，C조전이조여원발조조직중E-cadherin적표체차이무통계학의의(P=0.186)；β-catenin재B조여C조、C조원발조여전이조조직중적표체차이균무통계학의의(P=0.235、P=0.715)；C조E-cadherin포막적표체하조화β-catenin포막적표체하조현시정상관(r=0.387，P=O.024)，B조E-cadherin포막적표체하조화β-catenin포막적표체하조무명현상관성(r=0.080,P=0.723)。결론 E-Cadherin표체하조여결직장암반유림파결전이유관，차여β-catenin적표체하조유명현적상관성，이β-catenin표체여결직장암반림파결전이무관。
Objective To investigate the expression of E-cadherin and β-catenin in the primary colorectal carcinoma and metastatic lymph nodes and it's clinical significance. Methods Immunohistochemical analysis of Ecadherin and β-catenin was performed in colorectal mucosa( n =20 ,group A) ,colorectal carcinoma without metastasis ( n = 25, group B) and colorectal carcinoma with metastatic lymph nodes ( n = 39, group C) The expression of E-cadherin and β-catenin and the metastasis and prognosis of colorectal carcinoma were observed. Results The strongly positive expression of E-cadherin in primaries of group C (5.1％ ) was obviously lower than in group B ( U = 2. 149,P =0. 032) ,whereas no statistic differences of the expression of E-cadherin between primaries and metastases of group C were observed(P =0.186). There was no difference of the expression of β-catenin between group B and group C,the same of primary colorectal carcinoma and self control lymph nodes of group C(P =0.235 and P =0. 715). There was positive correlation between membranous down regulation of E-cadherin and β-catenin in group C( r = 0.387 ,P =0.024) ,but no relationship was found in group B( r = 0.080 ,P = 0.723 ). Conclusion The decreased membranous expression of E-cadherin in the colorectal carcinoma was correlated with lymph nodes metastasis, and was obviously related to membranous down regulation of β-catenin. There was no relevance between the expression of β-catenin and the lymph nodes metastasis of colorectal carcinoma.
