中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
3期
312-314
,共3页
孙传洋%臧亚晨%孙伟%陈岽%朱进%许立军%章良%薛波新%阳东荣%单玉喜
孫傳洋%臧亞晨%孫偉%陳崠%硃進%許立軍%章良%薛波新%暘東榮%單玉喜
손전양%장아신%손위%진동%주진%허립군%장량%설파신%양동영%단옥희
肾细胞癌%蛋白组学%分子标志物
腎細胞癌%蛋白組學%分子標誌物
신세포암%단백조학%분자표지물
Renal cell carcinoma%Proteomics%Molecular markersr
目的 分析肾细胞癌肿瘤特异性蛋白质,寻找新的肿瘤分子标志物.方法 应用二维电泳(2D-PAGE)分析9例肾细胞癌患者癌组织和周边的正常肾组织中差异表达的蛋白分子,并运用MALDI-TOF-MS和MS/MS法对差异表达的蛋白质标记点进行了鉴定.结果 经过二维电泳分离样本,发现了差异表达的16个蛋白质点(P＜0.05,t检验),用MALDI-TOF-MS法对这些点进行分析鉴定,确定了他们的种类,其中表达下调的有钙结合蛋白、酯类水解酶、乙醇脱氢酶、ammecr-1样蛋白、ADP/ATP转位-3和LYRM-5;表达上调的有HIG-1、谷胱甘肽S.转移酶(GSTP-1)、Peroxiredoxin-3、Peroxiredoxin-6、CD2相关蛋白(CD2AP)、Annexin V、γ-烯醇化酶、视网膜脱氢酶、波形蛋白(vimentin)和α-谷氨酰转移酶.2.结论 在筛选到的16种蛋白质中,钙结合蛋白、ADP/ATP转位酶-3、HIG-1、谷胱甘肽-S-转移酶、Peroxiredoxin-3和6是比较有研究价值的分子.
目的 分析腎細胞癌腫瘤特異性蛋白質,尋找新的腫瘤分子標誌物.方法 應用二維電泳(2D-PAGE)分析9例腎細胞癌患者癌組織和週邊的正常腎組織中差異錶達的蛋白分子,併運用MALDI-TOF-MS和MS/MS法對差異錶達的蛋白質標記點進行瞭鑒定.結果 經過二維電泳分離樣本,髮現瞭差異錶達的16箇蛋白質點(P＜0.05,t檢驗),用MALDI-TOF-MS法對這些點進行分析鑒定,確定瞭他們的種類,其中錶達下調的有鈣結閤蛋白、酯類水解酶、乙醇脫氫酶、ammecr-1樣蛋白、ADP/ATP轉位-3和LYRM-5;錶達上調的有HIG-1、穀胱甘肽S.轉移酶(GSTP-1)、Peroxiredoxin-3、Peroxiredoxin-6、CD2相關蛋白(CD2AP)、Annexin V、γ-烯醇化酶、視網膜脫氫酶、波形蛋白(vimentin)和α-穀氨酰轉移酶.2.結論 在篩選到的16種蛋白質中,鈣結閤蛋白、ADP/ATP轉位酶-3、HIG-1、穀胱甘肽-S-轉移酶、Peroxiredoxin-3和6是比較有研究價值的分子.
목적 분석신세포암종류특이성단백질,심조신적종류분자표지물.방법 응용이유전영(2D-PAGE)분석9례신세포암환자암조직화주변적정상신조직중차이표체적단백분자,병운용MALDI-TOF-MS화MS/MS법대차이표체적단백질표기점진행료감정.결과 경과이유전영분리양본,발현료차이표체적16개단백질점(P＜0.05,t검험),용MALDI-TOF-MS법대저사점진행분석감정,학정료타문적충류,기중표체하조적유개결합단백、지류수해매、을순탈경매、ammecr-1양단백、ADP/ATP전위-3화LYRM-5;표체상조적유HIG-1、곡광감태S.전이매(GSTP-1)、Peroxiredoxin-3、Peroxiredoxin-6、CD2상관단백(CD2AP)、Annexin V、γ-희순화매、시망막탈경매、파형단백(vimentin)화α-곡안선전이매.2.결론 재사선도적16충단백질중,개결합단백、ADP/ATP전위매-3、HIG-1、곡광감태-S-전이매、Peroxiredoxin-3화6시비교유연구개치적분자.
Objective To identify tumor specific proteins of renal cell carcinoma(RCC)by using proteomic analysis.Methods The expression of proteins was detected by silver stain after two-dimensional polyacrylamide gel electrophoresis(2D-PAGE).The differentially expressed proteins were identified by MALDI-TOF-MS.Results Six proteins were down-regulated and 10 proteins up-regulated in RCC tissues as compared with normal kidney tissues by silver stain after 2D-PAGE.The 16 differentially expressed protein spots(P＜0.05.by Student t-test)were identified by MALDl-TOF-MS and MS/MS.The down-regulated proteins included calbindin,ester hydrolase,alcohol dehydrogenase,ammecr-1-like protein,ADP/ATP tranglocase-3 and LYR motif-containing protein-5.and the up-regulated proteins included HIC-1 domain family member 1 A,glutathione-S-transferaae-P,thioredoxin-dependent-peroxide-reduetase,peroxiredoxin-6,CD2-associated protein,Annexin A5,gamma-enolase,retinal dehydrogenase-1,vimentin,and protein-gluramine gamma-glutamyltransferase-2.Conclusion Among the 16 proteins,Calbindin,ADP/ATP translocase3.HIG-1.glutathione-S-transferase-P-1,and thioredoxin-dependent-peroxide-reductase were worthy of further investigation.and maybe they will be developed into diagnostic markers for RCC.
