中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2010年
2期
144-149
,共6页
彭春林%江峰%王宝涛%杨晓惠%秦万章%许爱娥%孟炜
彭春林%江峰%王寶濤%楊曉惠%秦萬章%許愛娥%孟煒
팽춘림%강봉%왕보도%양효혜%진만장%허애아%맹위
红斑狼疮%系统性%T淋巴细胞%细胞毒性%细胞凋亡%多态性%单核苷酸
紅斑狼瘡%繫統性%T淋巴細胞%細胞毒性%細胞凋亡%多態性%單覈苷痠
홍반랑창%계통성%T림파세포%세포독성%세포조망%다태성%단핵감산
Lupus erythematosus%systemic%T-lymphocytes%cytotoxic%Apoptosis%Polymorphism%single nucleotide
目的 探讨中国长江以南汉族女性系统性红斑狼疮(SLE)患者细胞毒性T淋巴细胞相关抗原-4(CTLA-4)及程序性细胞凋亡-1(PDCD-1)基因多态与环境因素的交互作用.方法 采用单纯病例研究设计,收集258例符合条件的患者,应用聚合酶链反应-限制性片段长度多态(PCR-RFLP)技术检测CTLA-4与PDCD-1基因的单核苷酸多态性(SNPs),分别在显性、隐性、相加及相乘遗传模式下,选择Poisson对数线性模型估计基因-基因、基因-环境交互作用.结果 在PDCD-1基因的PD1.6位点,隐性及相加遗传模式下均存在GG基因型与紫外线暴露史的交互作用,OR值分别为3.714(95%CI:1.235～11.179)和3.199(95% CI:1.023～10.004).在CTLA-4-1722位点,显性遗传模式下,存在TT或TC基因型与紫外线暴露史的交互作用,OR值为4.874(95%CI:1.119～21.242);相乘遗传模式下存在PDCD-1基因的T等位基因与紫外线暴露史的交互作用,OR值为1.470(95%CI:1.047～2.065);而在相加遗传模式下,TT和TC基因型分别与紫外线暴露史存在交互作用,OR值分别为4.744(95% CI:1.037～21.737)和4.973(95% CI:1.110～22.287).结论 在中国长江以南汉族女性SLE患者中,紫外线暴露史分别与CTLA-4及PDCD-1基因多态存在交互作用,提示基因-环境交互作用可能与SLE有关.
目的 探討中國長江以南漢族女性繫統性紅斑狼瘡(SLE)患者細胞毒性T淋巴細胞相關抗原-4(CTLA-4)及程序性細胞凋亡-1(PDCD-1)基因多態與環境因素的交互作用.方法 採用單純病例研究設計,收集258例符閤條件的患者,應用聚閤酶鏈反應-限製性片段長度多態(PCR-RFLP)技術檢測CTLA-4與PDCD-1基因的單覈苷痠多態性(SNPs),分彆在顯性、隱性、相加及相乘遺傳模式下,選擇Poisson對數線性模型估計基因-基因、基因-環境交互作用.結果 在PDCD-1基因的PD1.6位點,隱性及相加遺傳模式下均存在GG基因型與紫外線暴露史的交互作用,OR值分彆為3.714(95%CI:1.235～11.179)和3.199(95% CI:1.023～10.004).在CTLA-4-1722位點,顯性遺傳模式下,存在TT或TC基因型與紫外線暴露史的交互作用,OR值為4.874(95%CI:1.119～21.242);相乘遺傳模式下存在PDCD-1基因的T等位基因與紫外線暴露史的交互作用,OR值為1.470(95%CI:1.047～2.065);而在相加遺傳模式下,TT和TC基因型分彆與紫外線暴露史存在交互作用,OR值分彆為4.744(95% CI:1.037～21.737)和4.973(95% CI:1.110～22.287).結論 在中國長江以南漢族女性SLE患者中,紫外線暴露史分彆與CTLA-4及PDCD-1基因多態存在交互作用,提示基因-環境交互作用可能與SLE有關.
목적 탐토중국장강이남한족녀성계통성홍반랑창(SLE)환자세포독성T림파세포상관항원-4(CTLA-4)급정서성세포조망-1(PDCD-1)기인다태여배경인소적교호작용.방법 채용단순병례연구설계,수집258례부합조건적환자,응용취합매련반응-한제성편단장도다태(PCR-RFLP)기술검측CTLA-4여PDCD-1기인적단핵감산다태성(SNPs),분별재현성、은성、상가급상승유전모식하,선택Poisson대수선성모형고계기인-기인、기인-배경교호작용.결과 재PDCD-1기인적PD1.6위점,은성급상가유전모식하균존재GG기인형여자외선폭로사적교호작용,OR치분별위3.714(95%CI:1.235～11.179)화3.199(95% CI:1.023～10.004).재CTLA-4-1722위점,현성유전모식하,존재TT혹TC기인형여자외선폭로사적교호작용,OR치위4.874(95%CI:1.119～21.242);상승유전모식하존재PDCD-1기인적T등위기인여자외선폭로사적교호작용,OR치위1.470(95%CI:1.047～2.065);이재상가유전모식하,TT화TC기인형분별여자외선폭로사존재교호작용,OR치분별위4.744(95% CI:1.037～21.737)화4.973(95% CI:1.110～22.287).결론 재중국장강이남한족녀성SLE환자중,자외선폭로사분별여CTLA-4급PDCD-1기인다태존재교호작용,제시기인-배경교호작용가능여SLE유관.
Objective To explore the interactions of gene polymorphisms of cytotoxie T lymphocyte antigen 4 (CTLA-4) and programmed cell death 1 (PDCD-1) with risk environmental factors in individuals with systemic lupus erythematosus (SLE) from Han nationality female population in South of Changjiang River region of China. Methods With a case-only design,a total of 258 cases were enrolled in this study, and single nucleotide polymorphisms(SNPs) of the PDCD-1 and CTLA-4 genes were determined by means of PCR-RFLP. With the aid of Poisson loglinear mode, interactions between gene-gene and gene-envimnment were fitted under the dominant, recessive, additive and multiple models, respectively. Results It was found that interaction existed between GG genotype of PD1.6 and UV history under separate inherent models of the recessive mode(OR = 3. 714,95% CI: 1. 235 - 11. 179) and additive mode (OR = 3. 199,95% CI: 1. 023 -10. 004). For CTLA-4 locus, there existed interactions between TT/TC genotype of -1722T→C and UV history under the dominant model (OR = 4. 874,95 % CI: 1.119 - 21. 242), and interaction between T allele and UV history was also found under the multiple model (OR = 1.470,95% CI: 1. 047 -2.065). While, under the additive mode for CTLA-4, it was found that interactions existed between TT genotype of -1722T→ C and UV history (OR =4. 744,95% CI: 1. 037 -21. 737), as well as between TC genotype of -1722T→C and UV history (OR =4. 973,95% CI:1. 110 -22. 287). Conclusion The interactions between UV history and polymorphisms of CTLA-4 and PDCD-1 gene for SLE were observed, which indicates that there may be association of their interactions with the development of SLE in Hart nationality females population in the south regions of Changjiang River in China.
