中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
12期
1812-1814
,共3页
王曦龙%陶中华%何洪卫%周嘉敏%汤钊猷%王鲁
王晞龍%陶中華%何洪衛%週嘉敏%湯釗猷%王魯
왕희룡%도중화%하홍위%주가민%탕쇠유%왕로
癌,肝细胞%干扰素-α%肝细胞生长因子%血管生成
癌,肝細胞%榦擾素-α%肝細胞生長因子%血管生成
암,간세포%간우소-α%간세포생장인자%혈관생성
Carcinoma,hepatocellular%IFN-α%Hepatocyte growth factor%Angiogenesis
目的 观察干扰素-α(IFN-α)对高转移潜能人肝癌裸鼠模型中血管内皮生长因子(VEGF)、肝细胞生长因子(HGF)mRNA表达的影响.方法 应用绿色荧光蛋白转染的人高转移肝癌细胞株HCC-LM3建立高转移潜能人肝癌裸鼠模型LCI-D20,种植后第2天开始分别使用IFN-α 1.5×104 U/(kg·d)(治疗组,n=12)或生理盐水(对照组,n=12)皮下注射,28 d后处死裸鼠,比较肝脏肿瘤的大小和肝内转移,免疫组织化学检测肿瘤微血管密度(MVD),实时荧光定量聚合酶链反应(PCR)检测肿瘤HGF和VEGF表达的变化.结果治疗组、对照组的肝内肿瘤大小分别为(0.11±0.03)cm3、(0.99±0.37)cm3(P<0.05);肝内转移率分别为33.3%(4/12)、83.3%(10/12)(P<0.05);肝内转移数目分别为0.67±0.31个比1.91±0.43个(P<0.05);肿瘤内MVD分别为3.19±0.52、4.85±0.72(P<0.05);肿瘤VEGF mRNA表达(-△CT)分别为-8.16±0.54、-6.95±0.86(P<0.05);HGF mRNA表达分别为-11.62±0.63、-10.56±0.48(P<0.05).结论 IFN-α对HGF及VEGF表达的抑制可能是其抗肿瘤血管生成作用、抑制肿瘤生长转移作用的机制之一.
目的 觀察榦擾素-α(IFN-α)對高轉移潛能人肝癌裸鼠模型中血管內皮生長因子(VEGF)、肝細胞生長因子(HGF)mRNA錶達的影響.方法 應用綠色熒光蛋白轉染的人高轉移肝癌細胞株HCC-LM3建立高轉移潛能人肝癌裸鼠模型LCI-D20,種植後第2天開始分彆使用IFN-α 1.5×104 U/(kg·d)(治療組,n=12)或生理鹽水(對照組,n=12)皮下註射,28 d後處死裸鼠,比較肝髒腫瘤的大小和肝內轉移,免疫組織化學檢測腫瘤微血管密度(MVD),實時熒光定量聚閤酶鏈反應(PCR)檢測腫瘤HGF和VEGF錶達的變化.結果治療組、對照組的肝內腫瘤大小分彆為(0.11±0.03)cm3、(0.99±0.37)cm3(P<0.05);肝內轉移率分彆為33.3%(4/12)、83.3%(10/12)(P<0.05);肝內轉移數目分彆為0.67±0.31箇比1.91±0.43箇(P<0.05);腫瘤內MVD分彆為3.19±0.52、4.85±0.72(P<0.05);腫瘤VEGF mRNA錶達(-△CT)分彆為-8.16±0.54、-6.95±0.86(P<0.05);HGF mRNA錶達分彆為-11.62±0.63、-10.56±0.48(P<0.05).結論 IFN-α對HGF及VEGF錶達的抑製可能是其抗腫瘤血管生成作用、抑製腫瘤生長轉移作用的機製之一.
목적 관찰간우소-α(IFN-α)대고전이잠능인간암라서모형중혈관내피생장인자(VEGF)、간세포생장인자(HGF)mRNA표체적영향.방법 응용록색형광단백전염적인고전이간암세포주HCC-LM3건립고전이잠능인간암라서모형LCI-D20,충식후제2천개시분별사용IFN-α 1.5×104 U/(kg·d)(치료조,n=12)혹생리염수(대조조,n=12)피하주사,28 d후처사라서,비교간장종류적대소화간내전이,면역조직화학검측종류미혈관밀도(MVD),실시형광정량취합매련반응(PCR)검측종류HGF화VEGF표체적변화.결과치료조、대조조적간내종류대소분별위(0.11±0.03)cm3、(0.99±0.37)cm3(P<0.05);간내전이솔분별위33.3%(4/12)、83.3%(10/12)(P<0.05);간내전이수목분별위0.67±0.31개비1.91±0.43개(P<0.05);종류내MVD분별위3.19±0.52、4.85±0.72(P<0.05);종류VEGF mRNA표체(-△CT)분별위-8.16±0.54、-6.95±0.86(P<0.05);HGF mRNA표체분별위-11.62±0.63、-10.56±0.48(P<0.05).결론 IFN-α대HGF급VEGF표체적억제가능시기항종류혈관생성작용、억제종류생장전이작용적궤제지일.
Objective To investigate the inhibitory effect of interferon ( IFN )-α on the hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) expression in nude mice orthotopically transplanted with human hepatocelllular carcinoma with highly metastatic potential.Methods A nude mice model with highly metastatic potential (LCI-D20) was established with HCC-LM3 transfected withgreen-fluorescent protein (GFP) gene in nude mice,One day after transplantation,nude mice were divided mal saline in control group,respectively.After 28 days,nude mice were sacrificed,the volume of tumor and intrahepatic metastases in two groups were compared.The microvessel density (MVD) of tumor was observed by immunohistochemistry.The expression of VEGF and HGF in tumor was detected by real-time reverse transcription-polymerase chain reaction (RT-PCR).Results The tumor sizes in treatment group and control group was (0.11 ± 0.03 ) and ( 0.99 ± 0.37) cm3,respectively ( P ＜ 0.05 ).The metastasis rate in treatment group and control group was 33.3% (4/12) and 83.3% ( 10/12),respectively (P＜0.05 ).The average number of metastatic nodules in treatment group and control group was 0.67 ± 0.31 and 1.91 ±0.43,respectively ( P ＜ 0.05 ).Intratumoral MVD in treatment group and control group was 3.19 ±0.52 and 4.85 ± 0.72,respectively (P ＜ 0.05 ).The VEGF expression ( -△CT) in treatment group and control group was -8.16 ±0.54 and -6.95 ±0.86,respectively (P＜0.05).The HGF expression in treatment group and control group was -11.62 ± 0.63 and - 10.56 ±0.48,respectively ( P ＜0.05).Conclusion IFN-α significantly inhibit growth of tumor and intrahepatic invasion HCC-LM3 xengraft modelas well as intratumoral MVD which might contribute to inhibition of VEGF and HGF expression by IFN-α.
