中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2012年
4期
284-290
,共7页
朱茗%鲁峰%高建华%廖云君
硃茗%魯峰%高建華%廖雲君
주명%로봉%고건화%료운군
血管基质层细胞%脂肪来源干细胞%脂肪移植
血管基質層細胞%脂肪來源榦細胞%脂肪移植
혈관기질층세포%지방래원간세포%지방이식
Stromal vascular fraction cells%Adipose-derived stem cells%Fat transplantation
目的 探讨应用自人脂肪组织新鲜分离的不同浓度的血管基质层细胞( stromal vasvular fraction cells,SVFs)辅助脂肪移植,从而提高移植物的存活率的方法.方法 脂肪组织来自临床行吸脂术者,提取SVFs,将0.3 ml待移植的脂肪颗粒分别与浓度为①5×105个/ml(A组)；②1 × 106个/ml(B组)；③2×106个/ml(C组)的SVFs混合,另设对照组为完全培养基+单纯脂肪颗粒(D组),随机注射移植于6只裸鼠背部皮下.术后3个月观察移植物情况:①湿重.②切片HE 染色计数血管密度.③方网测试系统“点计数”法检测存活脂肪细胞计数以及纤维坏死组织计数.结果 ①湿重:A组(60.000±6.325) mg,B组(81.670±7.528) mg,C组(68.330±7.528) mg,D 组(48.330±7.528) mg,B组脂肪存活率均高于A、C、D组(P<0.05),A、C2组之间比较差异无统计学意义(P＞0.05).②血管密度:A、B、C组血管密度均高于D组,且B组明显高于其他3组(P＜0.05),A、C2组之间比较差异无统计学意义(P＞0.05).③点计数:B组存活脂肪细胞计数均高于A、C、D组(P＜0.05),纤维组织计数均低于D组(P ＜0.05),A、C2组之间比较差异均无统计学意义(P＞ 0.05).结论 来源于人自体SVFs复合脂肪颗粒能够显著提高移植脂肪组织的成活率,其中1×106个/ml数量级的SVFs移植脂肪的存活率最高,更适合用于脂肪移植,具有广阔的临床应用前景.
目的 探討應用自人脂肪組織新鮮分離的不同濃度的血管基質層細胞( stromal vasvular fraction cells,SVFs)輔助脂肪移植,從而提高移植物的存活率的方法.方法 脂肪組織來自臨床行吸脂術者,提取SVFs,將0.3 ml待移植的脂肪顆粒分彆與濃度為①5×105箇/ml(A組)；②1 × 106箇/ml(B組)；③2×106箇/ml(C組)的SVFs混閤,另設對照組為完全培養基+單純脂肪顆粒(D組),隨機註射移植于6隻裸鼠揹部皮下.術後3箇月觀察移植物情況:①濕重.②切片HE 染色計數血管密度.③方網測試繫統“點計數”法檢測存活脂肪細胞計數以及纖維壞死組織計數.結果 ①濕重:A組(60.000±6.325) mg,B組(81.670±7.528) mg,C組(68.330±7.528) mg,D 組(48.330±7.528) mg,B組脂肪存活率均高于A、C、D組(P<0.05),A、C2組之間比較差異無統計學意義(P＞0.05).②血管密度:A、B、C組血管密度均高于D組,且B組明顯高于其他3組(P＜0.05),A、C2組之間比較差異無統計學意義(P＞0.05).③點計數:B組存活脂肪細胞計數均高于A、C、D組(P＜0.05),纖維組織計數均低于D組(P ＜0.05),A、C2組之間比較差異均無統計學意義(P＞ 0.05).結論 來源于人自體SVFs複閤脂肪顆粒能夠顯著提高移植脂肪組織的成活率,其中1×106箇/ml數量級的SVFs移植脂肪的存活率最高,更適閤用于脂肪移植,具有廣闊的臨床應用前景.
목적 탐토응용자인지방조직신선분리적불동농도적혈관기질층세포( stromal vasvular fraction cells,SVFs)보조지방이식,종이제고이식물적존활솔적방법.방법 지방조직래자림상행흡지술자,제취SVFs,장0.3 ml대이식적지방과립분별여농도위①5×105개/ml(A조)；②1 × 106개/ml(B조)；③2×106개/ml(C조)적SVFs혼합,령설대조조위완전배양기+단순지방과립(D조),수궤주사이식우6지라서배부피하.술후3개월관찰이식물정황:①습중.②절편HE 염색계수혈관밀도.③방망측시계통“점계수”법검측존활지방세포계수이급섬유배사조직계수.결과 ①습중:A조(60.000±6.325) mg,B조(81.670±7.528) mg,C조(68.330±7.528) mg,D 조(48.330±7.528) mg,B조지방존활솔균고우A、C、D조(P<0.05),A、C2조지간비교차이무통계학의의(P＞0.05).②혈관밀도:A、B、C조혈관밀도균고우D조,차B조명현고우기타3조(P＜0.05),A、C2조지간비교차이무통계학의의(P＞0.05).③점계수:B조존활지방세포계수균고우A、C、D조(P＜0.05),섬유조직계수균저우D조(P ＜0.05),A、C2조지간비교차이균무통계학의의(P＞ 0.05).결론 래원우인자체SVFs복합지방과립능구현저제고이식지방조직적성활솔,기중1×106개/ml수량급적SVFs이식지방적존활솔최고,경괄합용우지방이식,구유엄활적림상응용전경.
Objective To investigate the effect of different concentrations of human adipose stromal vascular fraction cells (SVFs) on the survival rate of fat transplantation.Methods 0.3 ml fat tissue,derived and refined from clinical liposuction patients.was mixed with different concentrations of SVFs as 5 × 105/ml in Group A,or 1 × 106/ml in Group B,or 2 × 106/ml in Group C,or completely medium in control group D.Then the mixture was injected randomly under the back skin of 6 nude mice.The transplanted fat tissue in four groups was harvested at 3 months after implantation.Wet weight of fat grafts was measured for macroscopic aspects.After HE staining,blood vessel density,viable adipocytes and fibrous proliferation were counted respectively for histological evaluation.Results The wet weight of fat grafts in group B(81.670 ± 7.528) mg was significantly higher than that in group A、C、D [ (60.000 ±6.325 ) mmg、(68.330 ± 7.528 ) mg、(48.330 ± 7.528 ) mg,respectively,P ＜ 0.05 ) ],but the difference between group A and group C was not statistically significant(P ＞ 0.05).The grafts in group A 、B and C had significantly higher blood vessel density than those in the control group D,whereas blood vessel density was the highest in group B( P ＜ 0.05 )and there was no significant difference between group A and C (P ＞0.05).Compared with group A,C and D,histological analysis revealed that the fat grafts in group B was consisted predominantly of adipose tissue with less fat necrosis and fibrosis (P < 0.05 ).However,fibrosis counts were significant lower in group A,B and C than those in group D( P <0.05),and there was no significant difference between group A and C(P ＞0.05).Conclusions The human isolated SVFs has the advantages to improve the survival rate of fat transplantation,and the magnitude of 1 × 106/ml is more practical and safe,indicating a wide clinical application in the future.
