遗传学报
遺傳學報
유전학보
ACTA GENETICA SINICA
2007年
5期
429-436
,共8页
胚乳%QTL作图%母体效应%两步等级设计
胚乳%QTL作圖%母體效應%兩步等級設計
배유%QTL작도%모체효응%량보등급설계
endosperm%QTL mapping%maternal effects%two-stage hierarchical design
文章提出了包括胚乳效应和母体效应的胚乳性状QTL定位的统计方法,该方法的实验设计是分子标记基因型信息来自F2母体植株和F3种子胚(或植株),胚乳性状表型值来自F3单粒种子胚乳,称之为两步等级设计.同时,用计算机全面模拟以验证该模型的可行性,模拟结果表明,只要群体足够大,该模型能较有效地进行胚乳性状QTL定位并精确地估计出胚乳QTL的各种遗传效应和母体效应.
文章提齣瞭包括胚乳效應和母體效應的胚乳性狀QTL定位的統計方法,該方法的實驗設計是分子標記基因型信息來自F2母體植株和F3種子胚(或植株),胚乳性狀錶型值來自F3單粒種子胚乳,稱之為兩步等級設計.同時,用計算機全麵模擬以驗證該模型的可行性,模擬結果錶明,隻要群體足夠大,該模型能較有效地進行胚乳性狀QTL定位併精確地估計齣胚乳QTL的各種遺傳效應和母體效應.
문장제출료포괄배유효응화모체효응적배유성상QTL정위적통계방법,해방법적실험설계시분자표기기인형신식래자F2모체식주화F3충자배(혹식주),배유성상표형치래자F3단립충자배유,칭지위량보등급설계.동시,용계산궤전면모의이험증해모형적가행성,모의결과표명,지요군체족구대,해모형능교유효지진행배유성상QTL정위병정학지고계출배유QTL적각충유전효응화모체효응.
A statistical method for mapping quantitative trait loci (QTLs) underlying endosperm traits is proposed. The method is based on a genetic model containing both the direct effects and maternal effects of an endosperm QTL and on an experimental design termed two-stage hierarchical design, in which the trait information is obtained from F3 endosperms and molecular marker information is obtained from F2 plants and F3 embryos (plants). Results of computer simulations indicate that the method can efficiently map endosperm QTLs and precisely estimate both the direct and maternal effects of endosperm QTLs when the sample size is sufficiently large.
