CAJ | 학술논문

目的:为广金钱草总黄酮胶囊制备含量测定用化学对照品.方法:采用常规柱层析结合制备型HPLC分离目标化合物,通过HPLC法测定其纯度和稳定性及其在胶囊中的含量,根据光谱数据(UV、IR、ESI-MS、1H-NMR、13C-NMR、DEPT、1H-13CCOSY、1H-1HCOSY、1D-HOHAHA、1D-NOE、HMBC)鉴定其结构.结果:分离到的化合物被鉴定为异夏佛塔苷,其纯度在99%以上,常温条件下放置3个月内稳定,其溶液在常温条件下8h内稳定.异夏佛塔苷在广金钱草总黄酮胶囊中含量为3.0%以上.结论:该化合物可作为广金钱草总黄酮胶囊的含量测定用对照品,并且能从广金钱草中分离得到.
목적:위엄금전초총황동효낭제비함량측정용화학대조품.방법:채용상규주층석결합제비형HPLC분리목표화합물,통과HPLC법측정기순도화은정성급기재효낭중적함량,근거광보수거(UV、IR、ESI-MS、1H-NMR、13C-NMR、DEPT、1H-13CCOSY、1H-1HCOSY、1D-HOHAHA、1D-NOE、HMBC)감정기결구.결과:분리도적화합물피감정위이하불탑감,기순도재99%이상,상온조건하방치3개월내은정,기용액재상온조건하8h내은정.이하불탑감재엄금전초총황동효낭중함량위3.0%이상.결론:해화합물가작위엄금전초총황동효낭적함량측정용대조품,병차능종엄금전초중분리득도.
AIM:To prepare a compound as the chemical reference substance of Guangjinqiancao Zonghuangtong Capsule.METHODS:To apply general column chromatography combined with preparative HPLC to isolate the target compound,to use analytic HPLC to determine the purity,stability and its content in the capsule,and to employ spectroscopic analysis (UV,IR,ESI-MS,1H-NMR,13C-NMR,DEPT,1H-13CCOSY,1H-1HCOSY,1DHOHAHA.1D.NOE,HMBC) to elucidate the structure of the isolated compound.RESULTS:The obtained compound was identified as isoschaftoside with the purity of over 99%, which was stable within 3 months at ambient temperature.As for isosehaftoside solution.it was stable within 8 h at ambient temperature.Its content in the capsule was above 3.0%.CONCLUSION:Isoschaftoside is a qualified reference substance for analytic assay ofGuangjinqiancao Zonghuangtong Capsule,and can be isolated from Desmodium styracifolium(Osb.)Merr.