CAJ | 학술논문

[目的]探讨天冬氨酸族氨基酸对沼泽红假单胞菌J001辅酶Q10合成的影响.[方法]通过在对数生长末期添加天冬氨酸族氨基酸来考察了其对沼泽红假单胞菌辅酶Q10合成的影响,以期为微生物发酵法获得大批量天然辅酶Q10的培养基优化和菌种的遗传改造提供依据.[结果]蛋氨酸的添加量与辅酶Q10合成呈正相关,蛋氨酸添加量达125 mg/L时,辅酶Q10产量达最大值23.8 mg/L,比对照提高20.2%;添加低浓度的赖氨酸、苏氨酸、异亮氨酸对辅酶Q10的合成没有影响,但高浓度(赖氨酸浓度达500 mg/L以上、苏氨酸400 mg/L以上、异亮氨酸400 mg/L以上)时,辅酶Q10的合成受到抑制,说明该菌株天冬氨酸激酶受到赖氨酸、苏氨酸以及异亮氨酸的反馈抑制或阻遏而不利于蛋氨酸的合成,进而减少辅酶Q10的产量.[结论]可通过菌种遗传改造获得抗赖氨酸、苏氨酸以及异亮氨酸结构类似物的反馈调节型和赖氨酸、苏氨酸以及异亮氨酸营养缺陷型突变体来提高辅酶Q10的产量.
[목적]탐토천동안산족안기산대소택홍가단포균J001보매Q10합성적영향.[방법]통과재대수생장말기첨가천동안산족안기산래고찰료기대소택홍가단포균보매Q10합성적영향,이기위미생물발효법획득대비량천연보매Q10적배양기우화화균충적유전개조제공의거.[결과]단안산적첨가량여보매Q10합성정정상관,단안산첨가량체125 mg/L시,보매Q10산량체최대치23.8 mg/L,비대조제고20.2%;첨가저농도적뢰안산、소안산、이량안산대보매Q10적합성몰유영향,단고농도(뢰안산농도체500 mg/L이상、소안산400 mg/L이상、이량안산400 mg/L이상)시,보매Q10적합성수도억제,설명해균주천동안산격매수도뢰안산、소안산이급이량안산적반궤억제혹조알이불리우단안산적합성,진이감소보매Q10적산량.[결론]가통과균충유전개조획득항뢰안산、소안산이급이량안산결구유사물적반궤조절형화뢰안산、소안산이급이량안산영양결함형돌변체래제고보매Q10적산량.
[Objective]The study aimed to investigate the effects of the amino acids of aspartate family on the biosynthesis of CoQ10 in Rhodpseudomonas palustris J001. [Method]The impacts of amino acids of this family on the biosynthesis of CoQ10 in Rhodopseudomnas palustris J001 were investigated by feeding these amino acids at the end of the logarithmic phase during incubation, which aim was for the optimization of the fermentation medium and genetic improvement of the strain for CoQ10 production. [Result]The results showed that feeding proper amount of methione (125 mg/L) could increase CoQ10 production by 20.2%, but feeding of lysine (above 500 mg/L), threonine (above 400 mg/L) and/or isoleucine (above 400 mg/L) repressed the biosynthesis of CoQ10. The results indicated that the aspartate kinase is subject to feedback inhibition or repression by lysine, threonine and isoleucine in the strain, which was unfavorable to the formation of methioine and then caused the decrease of CoQ10 production. [Conclusion]Lysine, threonine and isoleucine auxotrophic mutants with resistance to analogues of lysine, threonine and isoleucine could increase the production of CoQ10.