CAJ | 학술논문

目的:研究栀子苷、黄芩苷和小檗碱对缺氧.复氧损伤时大鼠脑微血管内皮细胞的保护机制.方法:建立离体大鼠脑微血管内皮细胞缺氧4 h复氧12 h损伤模型.用0.128,0.064,0.032 μmol·mL-1栀子苷,0.028,0.014,0.007 μmol.mL-1黄芩苷和0.024,0.012,0.006 μmol·mL-1小檗碱分别作用于损伤的细胞,采用免疫细胞化学方法和图象定量分析技术,检测平均吸光度和平均面积,比较各组血管细胞间黏附分子-1(VCAM-1)蛋白表达的变化;采用RT-PCR方法检测并比较各组VCAM-1 mRNA表达的变化.结果:模型组VCAM-1蛋白表达的平均吸光度值和平均面积以及VCAM-1 mRNA表达显著高于正常组(均P<0.01).所有给药组的平均吸光度值和平均面积均低于模型组,差异有统计学意义(均P<0.01).0.007 μmol·mL-1黄芩苷组、0.012,0.006 μmol.mL-1小檗碱组的平均吸光度值较正常组高,差异有统计学意义(P<0.05,P<0.01,P<0.01),其余各给药组与正常组的差异无统计学意义.0.014 μmol.mL-1黄芩苷组平均面积高于正常组,差异有统计学意义(P<0.05),其余各给药组与正常组的差异无统计学意义.各给药组mRNA表达高于正常组且低于模型组,差异均有统计学意义(P<0.05).结论:栀子苷、黄芩苷和小檗碱等黄连解毒汤有效成分能够保护缺氧-复氧损伤的大鼠脑微血管内皮细胞,其保护机制之一是抑制VCAM-1的表达.
목적:연구치자감、황금감화소벽감대결양.복양손상시대서뇌미혈관내피세포적보호궤제.방법:건립리체대서뇌미혈관내피세포결양4 h복양12 h손상모형.용0.128,0.064,0.032 μmol·mL-1치자감,0.028,0.014,0.007 μmol.mL-1황금감화0.024,0.012,0.006 μmol·mL-1소벽감분별작용우손상적세포,채용면역세포화학방법화도상정량분석기술,검측평균흡광도화평균면적,비교각조혈관세포간점부분자-1(VCAM-1)단백표체적변화;채용RT-PCR방법검측병비교각조VCAM-1 mRNA표체적변화.결과:모형조VCAM-1단백표체적평균흡광도치화평균면적이급VCAM-1 mRNA표체현저고우정상조(균P<0.01).소유급약조적평균흡광도치화평균면적균저우모형조,차이유통계학의의(균P<0.01).0.007 μmol·mL-1황금감조、0.012,0.006 μmol.mL-1소벽감조적평균흡광도치교정상조고,차이유통계학의의(P<0.05,P<0.01,P<0.01),기여각급약조여정상조적차이무통계학의의.0.014 μmol.mL-1황금감조평균면적고우정상조,차이유통계학의의(P<0.05),기여각급약조여정상조적차이무통계학의의.각급약조mRNA표체고우정상조차저우모형조,차이균유통계학의의(P<0.05).결론:치자감、황금감화소벽감등황련해독탕유효성분능구보호결양-복양손상적대서뇌미혈관내피세포,기보호궤제지일시억제VCAM-1적표체.