化学与生物工程
化學與生物工程
화학여생물공정
CHEMISTRY & BIOENGINEERING
2009年
7期
67-69
,共3页
钟为章%罗一菁%张忠智%鲁莽
鐘為章%囉一菁%張忠智%魯莽
종위장%라일정%장충지%로망
红螺菌科%光合细菌%平板菌落计数法%正交实验设计%液体培养基%优化
紅螺菌科%光閤細菌%平闆菌落計數法%正交實驗設計%液體培養基%優化
홍라균과%광합세균%평판균락계수법%정교실험설계%액체배양기%우화
Rhodospirillum branch%photosynthetic bacterium%plate count for bacterial colonies%orthogonal design%liquid medium%optimization
红螺菌科光合细菌液体培养基由乙酸钠、氯化铵、磷酸二氢钾、硫酸镁、酵母膏等5种原料组成.采用L16(45)正交表,得到液体培养基的优化配方为:乙酸钠3.3 g·L-1、氯化铵0.6 g·L-1、磷酸二氢钾0.9 g·L-1、硫酸镁0.5 g·L-1、酵母膏1.5 g·L-1,细菌在光照3000 lx、温度(32±2)℃条件下,培养3 d即可达到生长峰值,细菌总数从1.63×109 cfu·mL-1提高到3.68×109 cfu·mL-1.
紅螺菌科光閤細菌液體培養基由乙痠鈉、氯化銨、燐痠二氫鉀、硫痠鎂、酵母膏等5種原料組成.採用L16(45)正交錶,得到液體培養基的優化配方為:乙痠鈉3.3 g·L-1、氯化銨0.6 g·L-1、燐痠二氫鉀0.9 g·L-1、硫痠鎂0.5 g·L-1、酵母膏1.5 g·L-1,細菌在光照3000 lx、溫度(32±2)℃條件下,培養3 d即可達到生長峰值,細菌總數從1.63×109 cfu·mL-1提高到3.68×109 cfu·mL-1.
홍라균과광합세균액체배양기유을산납、록화안、린산이경갑、류산미、효모고등5충원료조성.채용L16(45)정교표,득도액체배양기적우화배방위:을산납3.3 g·L-1、록화안0.6 g·L-1、린산이경갑0.9 g·L-1、류산미0.5 g·L-1、효모고1.5 g·L-1,세균재광조3000 lx、온도(32±2)℃조건하,배양3 d즉가체도생장봉치,세균총수종1.63×109 cfu·mL-1제고도3.68×109 cfu·mL-1.
The liquid medium for Rhodospirillum branch of photosynthetic bacterium is composed of CH3COONa,NH4Cl,MgSO4,KH2PO4 and peptone. L16(45) Orthogonal design experiment was applied to optimize the culture medium,taking above 5 components as influencing factors on photosynthetic bacterium growth.According to the results,when the culture medium containing CH3COONa 3.3 g·L-1,NH4Cl 0.6 g·L-1,KH2PO4 0.9 g·L-1,MgSO4 0.5 g·L-1 and peptone 1.5 g·L-1,after 3 days growth under illumination of 3000 lx and temperature of (32±2)℃,the photosynthetic bacterium growth could reach the peak value.The total number of bacterium increased from initial 1.63×109 cfu·mL-1 to 3.68×109 cfu·mL-1.The optimized culture medium could effectively improve photosynthetic bacterium growth.
