CAJ | 학술논문

首先采用正交试验优化纤维素酶﹑果胶酶和木瓜蛋白酶对香菇(Lentinula edodes)子实体多糖酶解提取的工艺参数,然后在优化酶解条件下,依次采用纤维素酶﹑果胶酶和木瓜蛋白酶分步处理香菇子实体以提取香菇多糖,并与单一酶解提取法和传统热水浸提法进行对比.结果表明,纤维素酶﹑果胶酶﹑木瓜蛋白酶的最佳提取工艺参数依次为加酶量0.8%、温度45 ℃、pH 4.5、提取时间1 h,加酶量1.0%、温度45 ℃、pH 3.5、提取时间2.0 h和加酶量1.0%、温度45 ℃、pH 4.0、提取时间1.5 h;在优化提取条件下,分步酶解法提取香菇粗多糖的提取率可达14.17%,比传统热水浸提法提高128.2%,比单独采用纤维素酶﹑果胶酶﹑木瓜蛋白酶酶解提取分别提高了43.71%、46.99%和23.11%.紫外光谱分析表明,分步酶解法提取的香菇多糖纯度明显高于热水浸提法提取的香菇多糖.-
수선채용정교시험우화섬유소매﹑과효매화목과단백매대향고(Lentinula edodes)자실체다당매해제취적공예삼수,연후재우화매해조건하,의차채용섬유소매﹑과효매화목과단백매분보처리향고자실체이제취향고다당,병여단일매해제취법화전통열수침제법진행대비.결과표명,섬유소매﹑과효매﹑목과단백매적최가제취공예삼수의차위가매량0.8%、온도45 ℃、pH 4.5、제취시간1 h,가매량1.0%、온도45 ℃、pH 3.5、제취시간2.0 h화가매량1.0%、온도45 ℃、pH 4.0、제취시간1.5 h;재우화제취조건하,분보매해법제취향고조다당적제취솔가체14.17%,비전통열수침제법제고128.2%,비단독채용섬유소매﹑과효매﹑목과단백매매해제취분별제고료43.71%、46.99%화23.11%.자외광보분석표명,분보매해법제취적향고다당순도명현고우열수침제법제취적향고다당.-
The reaction conditions for enzymic extraction of polysaccharide from Lentinula edodes fruit bodies using cellulase, pectinase and papain individually were optimized using orthogonal testing L9(34). The respective optimal conditions for extraction with cellulase, pectinase and papain were as follows: (a) cellulose concentration 0.8%, temperature 45 ℃,pH 4.5,extraction time 1 h; (b) pectinase concentration 1.0%, temperature 45 ℃, pH 3.5,extraction time 2.0 h; (c) papain concentration 1.0%, temperature 45 ℃, pH 4.0,extraction time 1.5 h. Polysaccharide was then extracted using stepwise enzymic hydrolysis whereby each of the three enzymes was used successively under the respective optimal conditions. The traditional hot water extraction method was adopted as the control. The yield of polysaccharide extracted using stepwise enzymic hydrolysis (14.17%) was 128.2%, 43.71%, 46.99% and 23.11% higher, respectively compared with the individual yields obtained using hot water, cellulase, pectinase and papain. UV spectrophotometry revealed that the purity of the polysaccharide, as determined by Sevag's method, extracted using stepwise enzymic hydrolysis was higher compared to polysaccharide extracted using hot water.