CAJ | 학술논문

采用透射电镜技术,对水稻突变体B411"斑马叶"出现过程中叶片的叶绿体超微结构进行了研究.结果表明,在叶片包于叶鞘内的心叶期,叶片绿区叶绿体呈纺锤形,类囊体片层纵向整齐排列;黄区叶绿体呈不规则椭圆形,类囊体片层排列不规则.在叶片抽出叶鞘1 d的嫩叶期,叶片绿区叶绿体类囊体片层增多并垛叠形成基粒结构,它们沿长轴整齐排列;黄区叶绿体类囊体膜断裂,片断化.在成熟期,叶片绿区叶绿体类囊体片层变得丰富和形成许多基粒结构;叶片黄区叶绿体内部结构严重解体,整个叶绿体呈高电子密度的囊泡状结构.在复绿期,叶片黄区逐渐变成绿色,叶绿体结构恢复正常,类囊体膜系统重建,且有序地沿叶绿体长轴方向排列,在基质中形成淀粉粒,表明其光合能力恢复.
채용투사전경기술,대수도돌변체B411"반마협"출현과정중협편적협록체초미결구진행료연구.결과표명,재협편포우협초내적심협기,협편록구협록체정방추형,류낭체편층종향정제배렬;황구협록체정불규칙타원형,류낭체편층배렬불규칙.재협편추출협초1 d적눈협기,협편록구협록체류낭체편층증다병타첩형성기립결구,타문연장축정제배렬;황구협록체류낭체막단렬,편단화.재성숙기,협편록구협록체류낭체편층변득봉부화형성허다기립결구;협편황구협록체내부결구엄중해체,정개협록체정고전자밀도적낭포상결구.재복록기,협편황구축점변성록색,협록체결구회복정상,류낭체막계통중건,차유서지연협록체장축방향배렬,재기질중형성정분립,표명기광합능력회복.
A rice zebra leaf mutant B411 was examined using transmission electron microscopy technique to study the chloroplast ultrastrncture during development of zebra leaf. The results showed that there was a distinct difference between green and yellow zones of the same leaf when the leaf was enwrapped in the sheath. Chloroplasts were spindle-shaped with thylakoid membranes neatly arranged along the longitudinal direction in the green zone, however, chloroplasts in the yellow zone were oval-shaped with thylakoid membranes ranked irregularly. Along with zebra leaves shooting out of the sheath, difference between yellow zone and green zone in the same leaf became more and more evident, in the green zone, thylakoid membranes became rich, and piled up to be the grana. Nevertheless, thylakoid membrane fragmented, internal structure of chloroplast disaggregated seriously and had vesicle structures with high electron density in the yellow zone. With regreening in the yellow zone of zebra leaf, the shape of chloroplast in the yellow zone became spindle, and thylakoid membrane system reconstructed and gradually became rich, orderly arranging along with the long axis of the chloroplast, and some starch granules formed in the chloroplast which indicated that chloroplasts resumed the capacity of photosynthesis.