CAJ | 학술논문

先将毛泡桐叶片在MS,WPM,KM_8P和B_5等液体基本培养基中进行悬浮培养,然后建立毛泡桐叶片悬浮细胞培养及其体外植株再生体系.结果表明,毛泡桐叶片愈伤组织悬浮诱导最适培养基为改良MS+0.2 mg·L~(-1) NAA+8 mg·L~(-1)BA;细胞悬浮培养的最佳起始密度为6.67×10~6个·mL~(-1);悬浮愈伤组织芽诱导和根诱导的最适培养基分别为MS+0.3 mg·L~(-1)NAA+17 mg·L~(-1)BA和1/2MS+0.1 mg·L~(-1)NAA.
선장모포동협편재MS,WPM,KM_8P화B_5등액체기본배양기중진행현부배양,연후건립모포동협편현부세포배양급기체외식주재생체계.결과표명,모포동협편유상조직현부유도최괄배양기위개량MS+0.2 mg·L~(-1) NAA+8 mg·L~(-1)BA;세포현부배양적최가기시밀도위6.67×10~6개·mL~(-1);현부유상조직아유도화근유도적최괄배양기분별위MS+0.3 mg·L~(-1)NAA+17 mg·L~(-1)BA화1/2MS+0.1 mg·L~(-1)NAA.
The suspension cell line and system of its in vitro plantlet regeneration from the leaves of Paulownia tomentosa were established through optimization of the mineral and organic substances in MS media supplemented with various NAA and BA concentrations on the basis of callus induction rates in the basal liquid MS,WPM,KM8P and Bs.The results indicated that the optimal medium of callus in-duction from the leaves of Paulownia tomentosa were modified liquid MS +0.2 mg · L~(-1)NAA +8 mg·L~(-1) BA; and the best primary density for the cell culture was 6.67 × 10~6; The optimal ones of shoot and root induction from the induced calli in the modified liquid MS media were MS +0.3 mg · L~(-1) NAA + 17 mg · L~(-1)BA and 1/2MS +0.1 mg · L~(-1)NAA,respectively.