东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2010年
4期
98-103
,共6页
赵尔丰%高畅%高欣%程大海
趙爾豐%高暢%高訢%程大海
조이봉%고창%고흔%정대해
蓝莓果渣%花青素%酶-超声波辅助%提取工艺
藍莓果渣%花青素%酶-超聲波輔助%提取工藝
람매과사%화청소%매-초성파보조%제취공예
blueberry pomace%anthocyanins%enzymatic-ultrasonic-assisted%extraction procedure
以蓝莓果渣为原料,采用单因素试验法对提取工艺条件进行优化.确定了酶-超声波辅助提取蓝莓果渣中花青素的最佳工艺条件为:纤维素酶,温度50℃,酶用量5 mg·g-1,料液比1:10,超声波震荡培养提取功率200 W,时阃20 min,然后补充加入乙醇,使得体系中乙醇浓度为40%,超声波强化提取时间10 min.结果表明,酶辅助超声波提取法具有时间短、溶剂用量小、效率高等优点,可用于蓝莓花青素资源的最大化应用.
以藍莓果渣為原料,採用單因素試驗法對提取工藝條件進行優化.確定瞭酶-超聲波輔助提取藍莓果渣中花青素的最佳工藝條件為:纖維素酶,溫度50℃,酶用量5 mg·g-1,料液比1:10,超聲波震盪培養提取功率200 W,時閫20 min,然後補充加入乙醇,使得體繫中乙醇濃度為40%,超聲波彊化提取時間10 min.結果錶明,酶輔助超聲波提取法具有時間短、溶劑用量小、效率高等優點,可用于藍莓花青素資源的最大化應用.
이람매과사위원료,채용단인소시험법대제취공예조건진행우화.학정료매-초성파보조제취람매과사중화청소적최가공예조건위:섬유소매,온도50℃,매용량5 mg·g-1,료액비1:10,초성파진탕배양제취공솔200 W,시곤20 min,연후보충가입을순,사득체계중을순농도위40%,초성파강화제취시간10 min.결과표명,매보조초성파제취법구유시간단、용제용량소、효솔고등우점,가용우람매화청소자원적최대화응용.
The blueberry pomace was analyzed as the extracted material to study the optimum procedures of the enzymatic-ultrasonic-assisted extraction method.The single factor experiments were emplyed.The optimum procedures of the enzymatic-assisted ultrasonic extraction were as follows:cellulose,enzyme temperature 50℃,enzyme amounts 5 mg·g-1,solid to liquid ratio 1:10,ultrasonic incubao tion power 200 W,time 20 min.Afterwards,ethanol(final concentration 40%)was added to the mixture for ultrasonic-assisted extracting 20 min with the power of 250 W.The absorbance of anthocyanins extracts from blueberry pomace was the highest under these conditions.The method was time-saving.solvent saving and high extraction yield.so it can be used for the maximum of anthocyanins from blueberry resource.