CAJ | 학술논문

目的：研究牛膝多糖（ABPS）对人树突状细胞（DC）的功能的影响,为评价牛膝多糖对免疫系统功能的影响提供实验依据。方法：用浓度分别为0.1mmol/L、0.05mmol/L、0.025mmol/L的牛膝多糖处理成熟DC细胞72h,以CCK-8试剂检测各组DC的同种混合淋巴细胞反应（MLR）能力的差异;以ELISA双抗体夹心法检测各组DC分泌细胞因子IL-12p70、IL-17、TNF-α功能的差异。结果：与未处理组相比,各浓度牛膝处理组DC的同种MLR能力明显增强（p〈0.05）,尤其以0.1mmol/L处理组为甚。各浓度牛膝处理组DC的IL-12p70、IL-17、TNF-α的分泌能力有显著提高。结论：在本实验条件下,牛膝多糖对DC细胞因子的分泌及DC对同种T细胞的刺激能力具有一定的促进作用。
목적：연구우슬다당（ABPS）대인수돌상세포（DC）적공능적영향,위평개우슬다당대면역계통공능적영향제공실험의거。방법：용농도분별위0.1mmol/L、0.05mmol/L、0.025mmol/L적우슬다당처리성숙DC세포72h,이CCK-8시제검측각조DC적동충혼합림파세포반응（MLR）능력적차이;이ELISA쌍항체협심법검측각조DC분비세포인자IL-12p70、IL-17、TNF-α공능적차이。결과：여미처리조상비,각농도우슬처리조DC적동충MLR능력명현증강（p〈0.05）,우기이0.1mmol/L처리조위심。각농도우슬처리조DC적IL-12p70、IL-17、TNF-α적분비능력유현저제고。결론：재본실험조건하,우슬다당대DC세포인자적분비급DC대동충T세포적자격능력구유일정적촉진작용。
Objective：To study the effects induced by Achyranthes bidentata polysaccharides（APBS） on the functions of human dendritic cells（DC）,which could offer the experimental evidence to estimate the influence on the functions of human immune system by APBS.Methods： Human DC were stimulated by APBS for 72hours,with the concentration for 0.1mmol/L,0.05mmol/L and 0.025 mmol/L,respectively.CCK-8 kit was adopted to examine the function of allo-mixed lymphocyte reaction（allo-MLR） of DC,and Enzyme Linked Immunosorbent Assay（ELISA） to identify the levels of IL-12p70,IL-17 and TNF-α secreted by DC.Results： Compared with the sham APBS stimulated group,after stimulated by APBS,the ability of DC allo-MLR in each APBS treatment group was increased obviously（p0.05）,especially the group with the concentration of APBS for 0.1mmol/L.However,the secreted levels of IL-12p70,IL-17 and TNF-α of DC in each APBS treatment was increased obviously（p0.05）.Conclusion：Our study showed that APBS stimulation could somewhat up-regulate the secreted levels of IL-12p70,IL-17 and TNF-α and stimulation ability to allo-T cells of human DC.