CAJ | 학술논문

目的：探讨镉对原代培养大鼠睾丸支持细胞的毒性机制及黄芪甲苷的保护效果。方法：空白对照组、0.5%二甲基亚砜（DMSO）组、镉（50μmol/L）组、镉（50μmol/L）加黄芪甲苷（分别为5、10、20mg/L）组的培养支持细胞用于四甲基偶氮唑盐（MTT）细胞活性实验,DMSO组比较用于验证0.5%DMS0溶剂是否对细胞有毒害作用;镉（50μmol/L）加黄芪甲苷（分别为5、10、20mg/L）组用于筛选最佳保护作用的黄芪甲苷浓度。空白对照组、镉组、镉加最佳浓度黄芪甲苷组的培养细胞用于波形蛋白、E-钙粘连蛋白和β-环连蛋白的免疫组织化学（SABC法）检测。结果：24h和48h MTT细胞活性显示空白对照与DMSO组差异无统计学意义,镉加黄芪甲苷（10mg/L）组保护作用最好,镉组较空白对照组明显减弱;24h时镉加黄芪甲苷（10mg/L）组的细胞活性虽低于对照组但明显高于镉组;48h时镉加黄芪甲苷各组的细胞活性均高于镉组。镉处理后三种蛋白的阳性产物较对照组减弱,镉加黄芪甲苷（10mg/L）组较对照组减少但明显强于镉组。结论：黄芪甲苷可以保护镉致大鼠睾丸支持细胞的损伤作用。
목적：탐토력대원대배양대서고환지지세포적독성궤제급황기갑감적보호효과。방법：공백대조조、0.5%이갑기아풍（DMSO）조、력（50μmol/L）조、력（50μmol/L）가황기갑감（분별위5、10、20mg/L）조적배양지지세포용우사갑기우담서염（MTT）세포활성실험,DMSO조비교용우험증0.5%DMS0용제시부대세포유독해작용;력（50μmol/L）가황기갑감（분별위5、10、20mg/L）조용우사선최가보호작용적황기갑감농도。공백대조조、력조、력가최가농도황기갑감조적배양세포용우파형단백、E-개점련단백화β-배련단백적면역조직화학（SABC법）검측。결과：24h화48h MTT세포활성현시공백대조여DMSO조차이무통계학의의,력가황기갑감（10mg/L）조보호작용최호,력조교공백대조조명현감약;24h시력가황기갑감（10mg/L）조적세포활성수저우대조조단명현고우력조;48h시력가황기갑감각조적세포활성균고우력조。력처리후삼충단백적양성산물교대조조감약,력가황기갑감（10mg/L）조교대조조감소단명현강우력조。결론：황기갑감가이보호력치대서고환지지세포적손상작용。
Objective：To investigate the toxic mechanism of the cadmium（Cd）on rat Sertoli cell（Sc） by primary cultured and the protective effect of Astragaloside（A） to it.Methods：Sc were divided into control group,0.5%DMSO,Cd（50μmol/L） and Cd（5Oμmol/L）＋A（5,10,20mg/L）,then used for determination of the viability of Sc by MTT and immunohistochemistry of vimentin,E-cadherin,β-catenin by SABC method.Results：The viability of Sc were significantly decreased after cadmiumtreated（P0.05）;and it was higher in Cd＋A（10mg/L）group than Cd group after 24h,further,those in Cd＋A（5,10,20mg/L） groups were all higher than Cd group after 48h.Positive products of vimentin,E-cadherin and β-catenin in Cd group were obviously decreased,and those in Cd＋A group were lower than control group but higher than Cd group.Conclusion：A have antagonistic effect on cadmium-induced damage of rat Sc in vitro.