CAJ | 학술논문

CBF1转录激活因子是一类受低温诱导的反式作用因子，能有效提高植物抗低温的能力。本文通过PCR方法，以拟南芥叶片为材料，成功地克隆了CBF1转录因子基因，并将其连接到植物表达载体pB1121上，为通过农杆菌介导法转化牧草获得转基因植株奠定了基础。
CBF1전록격활인자시일류수저온유도적반식작용인자，능유효제고식물항저온적능력。본문통과PCR방법，이의남개협편위재료，성공지극륭료CBF1전록인자기인，병장기련접도식물표체재체pB1121상，위통과농간균개도법전화목초획득전기인식주전정료기출。
Transcription activator CBF1 is a trans-acting factor, which can be induced by low temperature. Derived from Arabidopsis, the CBF1 gene is Successfully cloned with PCR.Then CBF1 cDNA and plant expression vector pBI121 are conjugated to construct the recombinant plasmid pBI121-CBF1. Establish the foundation of introducing the arabidopsis transcriptional factor gene CBF1 into forage grass with agrobacterium mediated transgene technique.