CAJ | 학술논문

目的探讨2005-2007年深圳市H1N1流感病毒HA1基因变异特征.方法选取深圳市2005-2007年分离的H1N1流感毒株,提取病毒RNA,用RT-PCR扩增HA1区基因片段,产物纯化后测序并进行基因序列分析.结果 2005-2007年流感病毒分离率平均为7.16%,H1N1流感病毒在2005年和2006年的分离数占总分离数的比例分别为56.14%和66.03%,而2007年仅占3.61%.核苷酸同源性和基因进化树结果一致,2005年4月份之前分离株与A/New Caledonia/20/1999为同一分支,2005年5月份之后的分离株与A/Solomon Island/3/2006为一支,而2006-2007年分离株又与国家代表株A/GDLH/219/2006在一个分支.氨基酸序列分析显示,绝大多数的毒株均在第130位点缺失一个赖氨酸;2005年5月以后的大部分毒株出现以下氨基酸变异:T82K、Y94H、R146K、R209K、T267N,2006年5月份之后的毒株在抗原决定簇B区发生了A190T、H193Y、E195D氨基酸变异,同时也发生A区R146K的置换.但所有毒株的潜在糖基化和受体结合位点均比较保守.发现1株病毒A/SZ/68/2007具特殊性,经与参照毒株比较,其326个氨基酸中有50个发生变化,其中有11个位于抗原决定簇位点、6个位于受体结合位点,且有4个氨基酸变化导致糖基化位点丢失.结论 2005-2007年深圳市人群中至少有3个类型HA1基因不同的H1N1流感病毒株;由于氨基酸变异引起病毒发生抗原漂移,其代表株为A/GDLH/219/2006;发现的A/SZ/68/2007病毒毒株具有特殊性,其抗原特性和流行病学意义还有待探讨.
목적 탐토2005-2007년심수시H1N1류감병독HA1기인변이특정.방법 선취심수시2005-2007년분리적H1N1류감독주,제취병독RNA,용RT-PCR확증HA1구기인편단,산물순화후측서병진행기인서렬분석.결과 2005-2007년류감병독분리솔평균위7.16%,H1N1류감병독재2005년화2006년적분리수점총분리수적비례분별위56.14%화66.03%,이2007년부점3.61%.핵감산동원성화기인진화수결과일치,2005년4월빈지전분리주여A/New Caledonia/20/1999위동일분지,2005년5월빈지후적분리주여A/Solomon Island/3/2006위일지,이2006-2007년분리주우여국가대표주A/GDLH/219/2006재일개분지.안기산서렬분석현시,절대다수적독주균재제130위점결실일개뢰안산;2005년5월이후적대부분독주출현이하안기산변이:T82K、Y94H、R146K、R209K、T267N,2006년5월빈지후적독주재항원결정족B구발생료A190T、H193Y、E195D안기산변이,동시야발생A구R146K적치환.단소유독주적잠재당기화화수체결합위점균비교보수.발현1주병독A/SZ/68/2007구특수성,경여삼조독주비교,기326개안기산중유50개발생변화,기중유11개위우항원결정족위점、6개위우수체결합위점,차유4개안기산변화도치당기화위점주실.결론 2005-2007년심수시인군중지소유3개류형HA1기인불동적H1N1류감병독주;유우안기산변이인기병독발생항원표이,기대표주위A/GDLH/219/2006;발현적A/SZ/68/2007병독독주구유특수성,기항원특성화류행병학의의환유대탐토.
Objective To study the genetic and epidemiological characteristics of HA1 of influenza H1N1 viruses circulating in Shenzhen from 2005 to 2007. Methods The HA1 region was analyzed by RT-PCR and subsequently sequenced to analyze the HA1 genetic evolution. Phylogenetic analysis was confirmed on the homology of nucleitide comparing with the reference viruses of vaccines recommended by WHO and representative virus confirmed by China CDC. Relationship between isolation rates and genetic evolutions was explored. Results The average isolation rate from 2005 to 2007 was 7.16%. Of the isolates, the proportions of influenza H1N1 viruses in 2005, 2006 and 2007 were 56.14%, 66.03%,3.61% ,respectively. Data from HA1 phylogenetic analysis showed that there were at least three clades circulated in Shenzhen. Different viruses isolated during January to April were clustered with A/New Caledonia/20/1999 viruses isolated in the latter months of 2005 clustered with A/Solomon Island/3/2006 and viruses from 2006 to 2007 were in the same clade with A/GDLH/219/2006. Results showed that most viruses had a deletion of lysine at position 130. Compared with A/New Caledonia/20/1999, the virus isolated after May of 2005 occurred T82K, Y94H, R146K, R209K, T267N amino acid substitution, while some virus isolated after May 2006 took place the amino acid substitutions of A190T, H193Y,E195D (located at antigenic site B) and R146K(antigenic site A). The sequences at the receptor-binding sites and glycosylation sites were conserved. Compared with referring viruses, A/SZ/68/2007 had 50 amino acid substitutions in the HA1 region.Of these,eleven and six were located at antigenic sites and receptor-binding sites,respectively.Four amino acid substitution resulted in the deletion of glycosylation site.Conclusion Three different genetic lineages of influenza H1N1 virus were circulated in the population in Shenzhen during 2005-2007.The special virus named A/SZ/68/2007 should be paid further attention on its antigenic and epidemiological characteristics.