CAJ | 학술논문

目的研究PTEN蛋白在弥漫大B细胞淋巴瘤(DLBCL)中的表达情况,探讨PTEN蛋白表达与DLBCL预后的关系.方法采用免疫组化方法检测40例原发结内DLBCL患者肿瘤组织中PTEN蛋白的表达情况.应用Kaplan-Merie生存分析、Log-Rank差异显著性检验和Logistic回归分析进行统计学分析.结果 PTEN蛋白阳性组16例,阴性组24例.PTEN蛋白阳性组和阴性组患者的2年总体生存率差异无统计学意义(57.7%比62.5%,P>0.05),两组患者的生存率随时间的延长均逐渐减低,PTEN阳性组的生存率低于阴性组,但两组的生存曲线差异无统计学意义(P>0.05).PTEN蛋白表达与DLBCL分子分型无相关性(P>0.05).PTEN蛋白表达与患者年龄、体力状态、病情分期、乳酸脱氢酶(LDH)及结外受侵无相关性(P>0.05),回归分析表明,DLBCL中PTEN蛋白表达不是疾病的保护因素,国际预后指数(IPI)是疾病的危险因素(OR>1).此外,PTEN蛋白细胞核表达占15%(6/40),核表达的例数在PTEN蛋白表达阳性组和阴性组间差异无统计学意义(1:1).结论 DLBCL中PTEN蛋白表达高低与预后无相关性,IPI仍是判断其预后的指标.
목적 연구PTEN단백재미만대B세포림파류(DLBCL)중적표체정황,탐토PTEN단백표체여DLBCL예후적관계.방법 채용면역조화방법검측40례원발결내DLBCL환자종류조직중PTEN단백적표체정황.응용Kaplan-Merie생존분석、Log-Rank차이현저성검험화Logistic회귀분석진행통계학분석.결과 PTEN단백양성조16례,음성조24례.PTEN단백양성조화음성조환자적2년총체생존솔차이무통계학의의(57.7%비62.5%,P>0.05),량조환자적생존솔수시간적연장균축점감저,PTEN양성조적생존솔저우음성조,단량조적생존곡선차이무통계학의의(P>0.05).PTEN단백표체여DLBCL분자분형무상관성(P>0.05).PTEN단백표체여환자년령、체력상태、병정분기、유산탈경매(LDH)급결외수침무상관성(P>0.05),회귀분석표명,DLBCL중PTEN단백표체불시질병적보호인소,국제예후지수(IPI)시질병적위험인소(OR>1).차외,PTEN단백세포핵표체점15%(6/40),핵표체적례수재PTEN단백표체양성조화음성조간차이무통계학의의(1:1).결론 DLBCL중PTEN단백표체고저여예후무상관성,IPI잉시판단기예후적지표.
Objective To study PTEN protein expression and clinical significance in patients with diffuse large B cell lymphoma. Methods Immunohistochemical staining was used to determine the PTEN protein expression in 40 cases of primary diffuse large B lymphoma tissuse. The results were analyzed by Kaplan-Merie survival analysis, Log-Rank test and Logistic regression analysis. Results PTEN protein was positive in 16 cases and negative in 24 cases. There was no significant difference between two groups in twoyear overall survival rate(62.5 % vs 66.7 %, P >0.05). Survival analysis showed that patient' s survival time gradually were reduced with extended time between PTEN protein-positive group and negative group, lower in PTEN-positive group than the negative group, but there was no significant difference in survival curve (P >0.05) in the two groups. We compared characteristics of patients between PTEN protein positive and negative groups,including molecular type, patient' s age, stage, LDH, physical score and extranodular invasion, there was no significant difference among them. PTEN protein was not correlated with prognosis, while International Prognosis Index(IPI) was still a risk factor (OR >1). Conclusion PTEN protein expression may not predict the outcome in diffuse large B cell lymphoma, but IPI still is a predictor.