中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2011年
4期
403-404
,共2页
吴庆%吴静%贾汉·沙比提
吳慶%吳靜%賈漢·沙比提
오경%오정%가한·사비제
锌%砷%抗原,CD95%Fas配体蛋白质
鋅%砷%抗原,CD95%Fas配體蛋白質
자%신%항원,CD95%Fas배체단백질
Zinc%Arsenic%Antigens,CD95%Fas ligand protein
目的 探讨砷对小鼠肝脏Fas、FasL表达的影响及锌的拈抗作用.方法 选用健康昆明种小鼠60只,按体质量分成5组:阴性对照组(不给砷也不给锌),单纯给砷组(55 mg/L NaAsO2溶液),砷+低锌组、砷+中锌组、砷+高锌组均给55 mg/L NaAsO2溶液,并分别给20、40、80 mg/L ZnSO4溶液.经口连续灌胃(按体质量灌胃量为0.02 ml/g.1次/d)6周,待实验结束后处死小鼠,采用免疫组化法测定小鼠肝脏巾凋亡因子Fas、FasL表达.结果 干预组随着给锌量的增加,肝脏Fas、FasL的表达率逐渐降低.低、中锌+砷组的Fas的表达率[83.33%(10/12)、50%(6/12)]、FasL表达率[66.67%(8/12)、41.67%(5/12)]与阴性对照组Fas[8.33%(1/12)]、FasL[0.00%(0/12)]表达率比较,差异有统计学意义(P均<0.05);中、高锌+砷组的Fas的表达率[50%(6/12)、25%(3/12)]与阳性对照组[8.33%(1/12)]比较,差异有统计学意义(P均<0.01),中、高锌+砷组的FasL的表达率[41.67%(5/12)、16.67%(2/12)]与阳性对照组[91.67%(11/12)]比较,差异有统计学意义(P均<0.05).结论 砷可使小鼠肝脏Fas、FasL的表达率增高,促进肝脏的凋亡,锌可拮抗砷对细胞凋亡的作用.
目的 探討砷對小鼠肝髒Fas、FasL錶達的影響及鋅的拈抗作用.方法 選用健康昆明種小鼠60隻,按體質量分成5組:陰性對照組(不給砷也不給鋅),單純給砷組(55 mg/L NaAsO2溶液),砷+低鋅組、砷+中鋅組、砷+高鋅組均給55 mg/L NaAsO2溶液,併分彆給20、40、80 mg/L ZnSO4溶液.經口連續灌胃(按體質量灌胃量為0.02 ml/g.1次/d)6週,待實驗結束後處死小鼠,採用免疫組化法測定小鼠肝髒巾凋亡因子Fas、FasL錶達.結果 榦預組隨著給鋅量的增加,肝髒Fas、FasL的錶達率逐漸降低.低、中鋅+砷組的Fas的錶達率[83.33%(10/12)、50%(6/12)]、FasL錶達率[66.67%(8/12)、41.67%(5/12)]與陰性對照組Fas[8.33%(1/12)]、FasL[0.00%(0/12)]錶達率比較,差異有統計學意義(P均<0.05);中、高鋅+砷組的Fas的錶達率[50%(6/12)、25%(3/12)]與暘性對照組[8.33%(1/12)]比較,差異有統計學意義(P均<0.01),中、高鋅+砷組的FasL的錶達率[41.67%(5/12)、16.67%(2/12)]與暘性對照組[91.67%(11/12)]比較,差異有統計學意義(P均<0.05).結論 砷可使小鼠肝髒Fas、FasL的錶達率增高,促進肝髒的凋亡,鋅可拮抗砷對細胞凋亡的作用.
목적 탐토신대소서간장Fas、FasL표체적영향급자적념항작용.방법 선용건강곤명충소서60지,안체질량분성5조:음성대조조(불급신야불급자),단순급신조(55 mg/L NaAsO2용액),신+저자조、신+중자조、신+고자조균급55 mg/L NaAsO2용액,병분별급20、40、80 mg/L ZnSO4용액.경구련속관위(안체질량관위량위0.02 ml/g.1차/d)6주,대실험결속후처사소서,채용면역조화법측정소서간장건조망인자Fas、FasL표체.결과 간예조수착급자량적증가,간장Fas、FasL적표체솔축점강저.저、중자+신조적Fas적표체솔[83.33%(10/12)、50%(6/12)]、FasL표체솔[66.67%(8/12)、41.67%(5/12)]여음성대조조Fas[8.33%(1/12)]、FasL[0.00%(0/12)]표체솔비교,차이유통계학의의(P균<0.05);중、고자+신조적Fas적표체솔[50%(6/12)、25%(3/12)]여양성대조조[8.33%(1/12)]비교,차이유통계학의의(P균<0.01),중、고자+신조적FasL적표체솔[41.67%(5/12)、16.67%(2/12)]여양성대조조[91.67%(11/12)]비교,차이유통계학의의(P균<0.05).결론 신가사소서간장Fas、FasL적표체솔증고,촉진간장적조망,자가길항신대세포조망적작용.
Objective To investigate the effects of arsenic on liver Fas/FasL expression in mice and to observe the antagonize effect of zinc. Methods Sixty health Kunming mice were divided into five groups according to their body mass: negative control group(no arsenic and no zinc), arsenic group(55 mg/L NaAsO2 solution), low dose zinc intervention group(55 mg/L NaAsO2 solution and 20 mg/L ZnSO4 solution), middle dose zinc intervention group (55 mg/L NaAsO2 solution and 40 mg/L ZnSO4 solution), and high dose zinc intervention group (55 mg/LNaAsO2 solution and 80 mg/L ZnSO4 solution). Everyday the solution was given by oral gavage at a dose of 0.02 ml/g body weight for six weeks. The expression of Fas/FasL in mice liver was examined by immunohistochemistry.Results With the amount of zinc increasing, the expression of both Fas and FasL in mice liver decreased gradually. The expression rates of Fas[83.33%(10/12), 50%(6/12)] and FasL[66.67%(8/12), 41.67%(5/12)]in low dose zinc intervention group and middle dose zinc intervention group, respectively, were different from the expression rate of Fas[8.33%(1/12)] and FasL[0.00%(0/12)] in the negative control group(all P < 0.05). The Fas expression rate of middle dose zinc intervention group[50%(6/12)] and the high dose zinc intervention group [25%(3/12)] was compared with the arsenic group[8.33%(1/12)], and the difference was statistically significant (all P < 0.01 ). The FasL expression rate of the middle dose zinc intervention group [41.67% (5/12 )] and the high dose zinc intervention group[16.67%(2/12)] was compared with positive control group[91.67%(11/12)], and the difference was statistically significant (all P < 0.05). Conclusions Arsenic can increase the expression of Fas and FasL in mice liver, and promote apoptosis in liver, zinc may antagonize the effect of arsenic.