中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
38期
2667-2670
,共4页
陈适%曾正陪%李汉忠%童安莉%卢琳%宋爱羚%李明%梁伟%满娜%曹彩霞
陳適%曾正陪%李漢忠%童安莉%盧琳%宋愛羚%李明%樑偉%滿娜%曹綵霞
진괄%증정배%리한충%동안리%로림%송애령%리명%량위%만나%조채하
肾上腺肿瘤%瘦素%瘦素受体
腎上腺腫瘤%瘦素%瘦素受體
신상선종류%수소%수소수체
Adrenal gland neoplasms%Leptin%Leptin receptor
目的 观察长型瘦素受体(Ob-Rb)在正常肾上腺皮、髓质及其肿瘤中的mRNA和蛋白表达;并观察原发性醛固酮增多症(PA)、皮质醇分泌瘤(CS)和嗜铬细胞瘤(PHEO)患者的血浆瘦素水平.方法 提取6名健康者肾上腺皮质和髓质和10例CS、14例醛固酮分泌瘤(APA)、20例PHEO患者的组织总RNA和蛋白,用反转录-聚合酶链反应(RT-PCR)和Western印迹法检测不同组织中Ob-Rb mRNA和蛋白表达.ELISA方法测定29例PA、11例CS、15例PHEO患者和20名年龄匹配的血压健康者血浆瘦素水平.结果 在肾上腺皮、髓质及其肿瘤中存在Ob-Rb的mRNA和蛋白表达.在正常肾上腺皮质中,Ob-Rb mRNA(0.32±0.12)和蛋白(1.31±0.26)表达明显均高于其余各组(均P＜0.05).Ob-Rb mRNA在APA(0.15±0.10)中的表达明显高于CS(0.05±0.02,P＜0.05).APA中Ob-Rb mRNA表达与立位血浆醛固酮水平正相关(r=0.670,P=0.024),而CS患者瘤内Ob-RbmRNA水平与其24 h尿UFC水平正相关(r=0.870,P=0.005).CS患者血浆瘦素水平明显高于非皮质醇瘤患者(P=0.001).结论 肾上腺皮髓质及其肿瘤广泛表达长型瘦素受体,且其表达之间存在差异,瘦素与肾上腺间的关系还需进一步研究.
目的 觀察長型瘦素受體(Ob-Rb)在正常腎上腺皮、髓質及其腫瘤中的mRNA和蛋白錶達;併觀察原髮性醛固酮增多癥(PA)、皮質醇分泌瘤(CS)和嗜鉻細胞瘤(PHEO)患者的血漿瘦素水平.方法 提取6名健康者腎上腺皮質和髓質和10例CS、14例醛固酮分泌瘤(APA)、20例PHEO患者的組織總RNA和蛋白,用反轉錄-聚閤酶鏈反應(RT-PCR)和Western印跡法檢測不同組織中Ob-Rb mRNA和蛋白錶達.ELISA方法測定29例PA、11例CS、15例PHEO患者和20名年齡匹配的血壓健康者血漿瘦素水平.結果 在腎上腺皮、髓質及其腫瘤中存在Ob-Rb的mRNA和蛋白錶達.在正常腎上腺皮質中,Ob-Rb mRNA(0.32±0.12)和蛋白(1.31±0.26)錶達明顯均高于其餘各組(均P＜0.05).Ob-Rb mRNA在APA(0.15±0.10)中的錶達明顯高于CS(0.05±0.02,P＜0.05).APA中Ob-Rb mRNA錶達與立位血漿醛固酮水平正相關(r=0.670,P=0.024),而CS患者瘤內Ob-RbmRNA水平與其24 h尿UFC水平正相關(r=0.870,P=0.005).CS患者血漿瘦素水平明顯高于非皮質醇瘤患者(P=0.001).結論 腎上腺皮髓質及其腫瘤廣汎錶達長型瘦素受體,且其錶達之間存在差異,瘦素與腎上腺間的關繫還需進一步研究.
목적 관찰장형수소수체(Ob-Rb)재정상신상선피、수질급기종류중적mRNA화단백표체;병관찰원발성철고동증다증(PA)、피질순분비류(CS)화기락세포류(PHEO)환자적혈장수소수평.방법 제취6명건강자신상선피질화수질화10례CS、14례철고동분비류(APA)、20례PHEO환자적조직총RNA화단백,용반전록-취합매련반응(RT-PCR)화Western인적법검측불동조직중Ob-Rb mRNA화단백표체.ELISA방법측정29례PA、11례CS、15례PHEO환자화20명년령필배적혈압건강자혈장수소수평.결과 재신상선피、수질급기종류중존재Ob-Rb적mRNA화단백표체.재정상신상선피질중,Ob-Rb mRNA(0.32±0.12)화단백(1.31±0.26)표체명현균고우기여각조(균P＜0.05).Ob-Rb mRNA재APA(0.15±0.10)중적표체명현고우CS(0.05±0.02,P＜0.05).APA중Ob-Rb mRNA표체여립위혈장철고동수평정상관(r=0.670,P=0.024),이CS환자류내Ob-RbmRNA수평여기24 h뇨UFC수평정상관(r=0.870,P=0.005).CS환자혈장수소수평명현고우비피질순류환자(P=0.001).결론 신상선피수질급기종류엄범표체장형수소수체,차기표체지간존재차이,수소여신상선간적관계환수진일보연구.
Objective To compare the mRNA, protein expression of long leptin receptor (Ob-Rb)in human adrenal tissues and tumors and observe the plasma level of leptin in primary aldosteronism (PA),cortisol-secreting tumors (CS) and pheochromacytomas (PHEO). Methods Total RNA and protein were extracted from 6 normal human adrenal glands, 10 CS, 20 PHEO; and 14 aldosterone-producing adenomas (APA) (RNA), 10 APA (protein); plasma samples were drawn from 20 controls, 15 PHEO, 29 PA and 11 CS. Results The mRNA and protein of Ob-Rb were widely expressed in human adrenal glands and tumors. The mRNA (0. 32 ±0. 12) and protein (1.31 ±0. 26) expressions of Ob-Rb were higher in normal human adrenal cortex (C) than all other tissues (P ＜0. 05) while the mRNA expression of Ob-Rb in APA (0. 15 ± 0. 10) was higher than CS (0. 05 ± 0. 02) (P ＜ 0. 05). The mRNA expression of Ob-Rb in APA was positively correlated with plasma supine aldosterone level (r= 0. 670, P= 0. 024). The mRNA expression of Ob-Rb in CS was positively correlated with 24-hour urinary free cortisol level(r=0. 870, P=0. 005). The plasma level of leptin was higher in CS than in non-CS groups (P= 0. 001). Conclusions Ob-Rb is widely expressed in adrenal tissues and tumors. There is a differential expression in various tissues. Further studies are warranted to understand the relationship between leptin and adrenal gland.
