中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
1期
51-55
,共5页
熊怡淞%朱烨%陆慧琦%李畅%韩志君%仲人前
熊怡淞%硃燁%陸慧琦%李暢%韓誌君%仲人前
웅이송%주엽%륙혜기%리창%한지군%중인전
动脉粥样硬化%氧化型低密度脂蛋白%Siglec-1%RAW264.7
動脈粥樣硬化%氧化型低密度脂蛋白%Siglec-1%RAW264.7
동맥죽양경화%양화형저밀도지단백%Siglec-1%RAW264.7
Atherosclerosis%Oxidized low-density lipopretein(ox-LDL)%Siglec-1%RAW264.7
目的 通过氧化型低密度脂蛋白(oxidized low-density lipopretein,ox-LDL)活化巨噬细胞系对Siglec-1(sialic acid-binding immunoglobuhn-like herin 1,唾液酸黏附素)表达及细胞因子分泌的影响探讨对动脉粥样硬化(atherosclerosis,AS)发病的可能作用.方法 制备ox-LDL,并用不同浓度ox-LDL刺激小鼠巨噬细胞株RAW264.7,48 h后收集细胞和上清,分别用流式细胞仪和RT-PCR检测不同浓度ox-LDL刺激后细胞表面Siglec-1蛋白和基因表达水平,并测定培养上清液中巨噬细胞炎性蛋白-1α(MIP-1α)、单核细胞趋化蛋白-1(MCP-1)和IL-8的浓度.结果 与对照组相比,ox-LDL能刺激RAW264.7细胞表面Siglec-1蛋白和mRNA表达升高(P<0.01),培养上清液中MIP-1α、MCP-1和IL-8的表达升高(P<0.01),并呈浓度依赖性.结论 ox-LDL可呈剂量依赖性刺激RAW264.7巨噬细胞诱导Siglec-1表达及分泌炎性细胞因子增加,在动脉粥样硬化的发生发展中可能起一定作用.
目的 通過氧化型低密度脂蛋白(oxidized low-density lipopretein,ox-LDL)活化巨噬細胞繫對Siglec-1(sialic acid-binding immunoglobuhn-like herin 1,唾液痠黏附素)錶達及細胞因子分泌的影響探討對動脈粥樣硬化(atherosclerosis,AS)髮病的可能作用.方法 製備ox-LDL,併用不同濃度ox-LDL刺激小鼠巨噬細胞株RAW264.7,48 h後收集細胞和上清,分彆用流式細胞儀和RT-PCR檢測不同濃度ox-LDL刺激後細胞錶麵Siglec-1蛋白和基因錶達水平,併測定培養上清液中巨噬細胞炎性蛋白-1α(MIP-1α)、單覈細胞趨化蛋白-1(MCP-1)和IL-8的濃度.結果 與對照組相比,ox-LDL能刺激RAW264.7細胞錶麵Siglec-1蛋白和mRNA錶達升高(P<0.01),培養上清液中MIP-1α、MCP-1和IL-8的錶達升高(P<0.01),併呈濃度依賴性.結論 ox-LDL可呈劑量依賴性刺激RAW264.7巨噬細胞誘導Siglec-1錶達及分泌炎性細胞因子增加,在動脈粥樣硬化的髮生髮展中可能起一定作用.
목적 통과양화형저밀도지단백(oxidized low-density lipopretein,ox-LDL)활화거서세포계대Siglec-1(sialic acid-binding immunoglobuhn-like herin 1,타액산점부소)표체급세포인자분비적영향탐토대동맥죽양경화(atherosclerosis,AS)발병적가능작용.방법 제비ox-LDL,병용불동농도ox-LDL자격소서거서세포주RAW264.7,48 h후수집세포화상청,분별용류식세포의화RT-PCR검측불동농도ox-LDL자격후세포표면Siglec-1단백화기인표체수평,병측정배양상청액중거서세포염성단백-1α(MIP-1α)、단핵세포추화단백-1(MCP-1)화IL-8적농도.결과 여대조조상비,ox-LDL능자격RAW264.7세포표면Siglec-1단백화mRNA표체승고(P<0.01),배양상청액중MIP-1α、MCP-1화IL-8적표체승고(P<0.01),병정농도의뢰성.결론 ox-LDL가정제량의뢰성자격RAW264.7거서세포유도Siglec-1표체급분비염성세포인자증가,재동맥죽양경화적발생발전중가능기일정작용.
Objective To explore the role of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1) in the process of atherosclerotic inflammation induced by oxidized low-density hpepmtein (ox-LDL). Methods Ox-LDL was synthesized by oxidization of native LDL and different concentration of ox-LDL was added to the culture medium of RAW264.7. Forty-eight hours later, cells and supernatants were collected separately. The expression of Siglec-1 protein and mRNA were measured by flow cytometry(FCM) and real-time quantitative RT-PCR, respectively. The levels of monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1α(MIP-1α) and IL-8 in supernatants were determined by ELISA. Re-sults By the stimulation of ox-LDL, Siglec-1 protein and mRNA on RAW264.7 cells were significantly in-creased, meanwhile, the cytokines levels in culture supematants were significantly higher than that in the control group. And both Siglec-1 expression and cytokine secretion were ox-LDL dose-dependent. Conclu-sion Ox-LDL can increase Siglec-1 protein and mRNA expression and some inflammatory cytokines secre-tion on RAW264.7 cells in a dose-dependent manner. Manifested by enhanced Siglec-1 expression, the acti-vated macmphages may take a part in the development and progression of atherosclerosis.
