中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2009年
1期
21-25
,共5页
李贵刚%张虹%刘荣%夏瑜%毕江江
李貴剛%張虹%劉榮%夏瑜%畢江江
리귀강%장홍%류영%하유%필강강
高眼压%受体,糖皮质激素%白细胞,单核
高眼壓%受體,糖皮質激素%白細胞,單覈
고안압%수체,당피질격소%백세포,단핵
Ocular hypertension%Receptors,glucocorticoid%Leukocytes,mononuclear
目的 探讨糖皮质激素性高眼压(GIOH)患者外周血单个核细胞(PBMCs)中糖皮质激素受体(GR)亚型GRα和GRβ的表达及其与GIOH发病的关系.方法 病例对照研究.共有32例GIOH患者接受PBMCs检测,原发疾病为过敏性结膜炎.根据GIOH患者局部使用糖皮质激素滴眼液(0.1%地塞米松)后眼压变化水平,分为GIOH组[眼压升高8 mm Hg(1 mln Hg=0.133 kPa)以上]和对照组(眼压升高不足8 mm Hg),每组各16例.采用半定量逆转录-聚合酶链反应(RT-PCR)法,检测GIOH组和对照组患者PBMCs中GRα mRNA、GRβ mRNA表达水平(以半定量比值表示);采用荧光标记流式细胞技术,检测GIOH组与对照组患者PBMCs中GRα和GRβ蛋白含量(以细胞阳性率表示).应用SPSS13.0统计学软件,对组间患者PBMCs中GRα mRNA和GRβ mRNA表达半定量比值和细胞阳性率进行比较,采用t检验,以P<0/05作为差异有统计学意义.结果 RT-PCR法检测,GIOH组患者PBMCs中GRα mRNA半定量比值为(1.152±0.057),与对照组(1.048±0.031)相比明显增高,差异有统计学意义(t=3.872,P<0.05);GIOH组患者PBMCs中GRβ mRNA半定量比值为(1.055±0.034),与对照组(1.063±0.035)相比,差异无统计学意义(t=0.419,P>0.05).荧光标记流式细胞技术检测,GIOH组患者PBMCs中GRα蛋白表达阳性率为(93.10±7.35)%,对照组为(46.00±13.11)%,两组间差异有统计学意义(t=8.513,P<0.01);GIOH组患者PBMCs中GRB蛋白表达阳性率为(12.50±4.18)%,对照组为(23.83±3.92)%,两组间差异有统计学意义(t=4.842,P<0.01).结论 GIOH组患者PBMCs中GRα mRNA和蛋白表达水平高于非高眼压患者,GRβmRNA和蛋白表达水平低于非高眼压患者,提示PBMCs中GR mRNA和蛋白表达水平可能与GIOH的易感性有关.(中华眼科杂志,2009,45:21-25)
目的 探討糖皮質激素性高眼壓(GIOH)患者外週血單箇覈細胞(PBMCs)中糖皮質激素受體(GR)亞型GRα和GRβ的錶達及其與GIOH髮病的關繫.方法 病例對照研究.共有32例GIOH患者接受PBMCs檢測,原髮疾病為過敏性結膜炎.根據GIOH患者跼部使用糖皮質激素滴眼液(0.1%地塞米鬆)後眼壓變化水平,分為GIOH組[眼壓升高8 mm Hg(1 mln Hg=0.133 kPa)以上]和對照組(眼壓升高不足8 mm Hg),每組各16例.採用半定量逆轉錄-聚閤酶鏈反應(RT-PCR)法,檢測GIOH組和對照組患者PBMCs中GRα mRNA、GRβ mRNA錶達水平(以半定量比值錶示);採用熒光標記流式細胞技術,檢測GIOH組與對照組患者PBMCs中GRα和GRβ蛋白含量(以細胞暘性率錶示).應用SPSS13.0統計學軟件,對組間患者PBMCs中GRα mRNA和GRβ mRNA錶達半定量比值和細胞暘性率進行比較,採用t檢驗,以P<0/05作為差異有統計學意義.結果 RT-PCR法檢測,GIOH組患者PBMCs中GRα mRNA半定量比值為(1.152±0.057),與對照組(1.048±0.031)相比明顯增高,差異有統計學意義(t=3.872,P<0.05);GIOH組患者PBMCs中GRβ mRNA半定量比值為(1.055±0.034),與對照組(1.063±0.035)相比,差異無統計學意義(t=0.419,P>0.05).熒光標記流式細胞技術檢測,GIOH組患者PBMCs中GRα蛋白錶達暘性率為(93.10±7.35)%,對照組為(46.00±13.11)%,兩組間差異有統計學意義(t=8.513,P<0.01);GIOH組患者PBMCs中GRB蛋白錶達暘性率為(12.50±4.18)%,對照組為(23.83±3.92)%,兩組間差異有統計學意義(t=4.842,P<0.01).結論 GIOH組患者PBMCs中GRα mRNA和蛋白錶達水平高于非高眼壓患者,GRβmRNA和蛋白錶達水平低于非高眼壓患者,提示PBMCs中GR mRNA和蛋白錶達水平可能與GIOH的易感性有關.(中華眼科雜誌,2009,45:21-25)
목적 탐토당피질격소성고안압(GIOH)환자외주혈단개핵세포(PBMCs)중당피질격소수체(GR)아형GRα화GRβ적표체급기여GIOH발병적관계.방법 병례대조연구.공유32례GIOH환자접수PBMCs검측,원발질병위과민성결막염.근거GIOH환자국부사용당피질격소적안액(0.1%지새미송)후안압변화수평,분위GIOH조[안압승고8 mm Hg(1 mln Hg=0.133 kPa)이상]화대조조(안압승고불족8 mm Hg),매조각16례.채용반정량역전록-취합매련반응(RT-PCR)법,검측GIOH조화대조조환자PBMCs중GRα mRNA、GRβ mRNA표체수평(이반정량비치표시);채용형광표기류식세포기술,검측GIOH조여대조조환자PBMCs중GRα화GRβ단백함량(이세포양성솔표시).응용SPSS13.0통계학연건,대조간환자PBMCs중GRα mRNA화GRβ mRNA표체반정량비치화세포양성솔진행비교,채용t검험,이P<0/05작위차이유통계학의의.결과 RT-PCR법검측,GIOH조환자PBMCs중GRα mRNA반정량비치위(1.152±0.057),여대조조(1.048±0.031)상비명현증고,차이유통계학의의(t=3.872,P<0.05);GIOH조환자PBMCs중GRβ mRNA반정량비치위(1.055±0.034),여대조조(1.063±0.035)상비,차이무통계학의의(t=0.419,P>0.05).형광표기류식세포기술검측,GIOH조환자PBMCs중GRα단백표체양성솔위(93.10±7.35)%,대조조위(46.00±13.11)%,량조간차이유통계학의의(t=8.513,P<0.01);GIOH조환자PBMCs중GRB단백표체양성솔위(12.50±4.18)%,대조조위(23.83±3.92)%,량조간차이유통계학의의(t=4.842,P<0.01).결론 GIOH조환자PBMCs중GRα mRNA화단백표체수평고우비고안압환자,GRβmRNA화단백표체수평저우비고안압환자,제시PBMCs중GR mRNA화단백표체수평가능여GIOH적역감성유관.(중화안과잡지,2009,45:21-25)
Objective To study the expression of glucocorticoid receptors (α and β) in mononuclear cells of peripheral blood (PBMCs) in patients with glucocorticoid-induced ocular hypertension (GIOH) and the relationship between of the expression of GRα and GRβ in PBMCs and its susceptibility to GIOH. Methods Case control study. Thirty-two patients with anaphylactic conjunctivitis were enrolled in this study. All of whom were received topical application of 0.1% dexamethasone. The patients with the elevation of intraocular pressure (IOP) over 8 mm Hg(1 mm Hg =0.133 kPa) or higher within two weeks were defined as GIOH and the others were considered as control group. There were no statistical difference in sex, average age, and baseline IOP between two groups. Each group consisted of sixteen patients. GRα and GRβ mRNA (relative value) in PBMCs were detected with semi-quantitative reverse transcriptional polymerase chain reaction (RT-PCR), GRα and GRβ protein (positive ratio) in PBMCs examined with fluorescent labelling flow cytometry. Results The GRα mRNA/GAPDH ratio of GIOH group was significantly (t=3.872,P<0.05) higher than that of control group (1.152±0.057 vs 1.048±0.031). The GRβ mRNA/GAPDH of GIOH group was lower than that of control group's (1.055±0.034 vs 1.063± 0.035), but was not statistically different (t=0.419, P>0.05). GRα positive percentage was significantly (t=8.513, P<0.01) higher in PBMCs of GIOH group than that of the control group (93.10±7.35) % vs (46.00±13.11) %, whereas GRβ positive percentage was significantly (t=4.842 P<0.01) lower in PBMCs of GIOH group than that in control group (12.50±4.18) % vs (23.83±3.92) %. Conclusions The higher expression of GRα in PBMCs in patients may have an implication of susceptibility to GIOH and warrant further investigation. (Chin J Ophthalmol, 2009,45:21-25)
