CAJ | 학술논문

将含有两个35S启动子和4个串联重复的35S增强子的Ac/Ds双元转座子标签载体转入水稻(Oryza sativaL.)基因组.100个转化植株的分析结果表明,Ds在T0植株的体细胞中切离频率高达60%.20个T1株系的600个T1植株的PCR分析结果表明,14个株系(70%)表现出较高的切离频率(约43%～100%).Southern杂交发现在同一T1株系的不同植株中Ds转座既有相同的转座位点(约90%),也有不同的转座位点(约10%).
장함유량개35S계동자화4개천련중복적35S증강자적Ac/Ds쌍원전좌자표첨재체전입수도(Oryza sativaL.)기인조.100개전화식주적분석결과표명,Ds재T0식주적체세포중절리빈솔고체60%.20개T1주계적600개T1식주적PCR분석결과표명,14개주계(70%)표현출교고적절리빈솔(약43%～100%).Southern잡교발현재동일T1주계적불동식주중Ds전좌기유상동적전좌위점(약90%),야유불동적전좌위점(약10%).
A two-component Ac/Ds activation tagging system, with two 35S promoters and four tandem repeats of the enhancer fragment of this promoter within the Ds element, was introduced into rice (Oryza sativa L.) genome. The results from 100 T0transgenic plants showed that the somatic excision frequencies of Ds were high to 60%. Fourteen out of 20 T1 plant lines showed excision frequencies between approximately 43% and 100%. Southern hybridization analysis revealed that the same bands and the different bands were observed between different plants of the same T1 line and the different bands were about 10% in all analyzed T1 plant lines.