中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2009年
50期
9874-9878
,共5页
王维群%陆彩凤%姜文凯%雷涛%潘菊芳
王維群%陸綵鳳%薑文凱%雷濤%潘菊芳
왕유군%륙채봉%강문개%뢰도%반국방
血管内皮细胞生长因子%低氧%低练高住%运动强度
血管內皮細胞生長因子%低氧%低練高住%運動彊度
혈관내피세포생장인자%저양%저련고주%운동강도
背景:通过运动和,或低氧来增加机体对低氧的应激程度和时间,可提高机体对运动和,或低氧的适应水平,但目前对急性运动和,或低氧对骨骼肌血管内皮生长因子表达的影响所知甚少.目的:观察急性运动和,或低氧对大鼠腓肠肌血管内皮生长因子表达的影响.设计、时间及地点:随机对照动物实验,于2005-09/2006-09在江苏省人民医院临床实验研究室完成.对象:选用健康雄性SD大鼠108只,随机分为6组:即常氧安静组、急性常氧高强度组、急性常氧中强度组、低氧安静组、急性低练高住高强度组和急性低练高住中强度组,每组18只.段氏PT型鼠跑台为杭州产.实验方法:急性常氧运动模型:运动前48 h进行适应活动.高强度运动:运动50 m/min×1.5 min休2 min.中强度运动:运动30 m/min×30 min.低氧模型:采用低氧仪产生低氧气分压的混合气体,低氧3 d,22 h/d,氧气12.8%,温度22℃,湿度55%.急性低练高住模型:在急性高、中强度运动后实施上述低氧计划.于各组处理后即刻、2,4 h分别处死大鼠4只取大鼠腓肠肌.主要观察指标:用Western-blot法测定大鼠腓肠肌血管内皮生长因子表达.结果:低氧和急性常氧运动增强了血管内皮生长因子表达,运动后低氧削弱了运动诱导的血管内皮生长因子表达,长时间中等强度的运动诱导更多的血管内皮生长因子表达.急性运动和,或低氧后即刻、2 h血管内皮生长因子表达最多,其恢复速度由快向慢排序为:低氧或低练高住、常氧运动.结论:急性运动和,或低氧诱导的大鼠骨骼肌血管内皮生长因子的表达属早期速发效应,且存在强度阈值,其恢复速度与表达的幅度成反比,低练高住可能能增强骨骼肌对运动的适应.
揹景:通過運動和,或低氧來增加機體對低氧的應激程度和時間,可提高機體對運動和,或低氧的適應水平,但目前對急性運動和,或低氧對骨骼肌血管內皮生長因子錶達的影響所知甚少.目的:觀察急性運動和,或低氧對大鼠腓腸肌血管內皮生長因子錶達的影響.設計、時間及地點:隨機對照動物實驗,于2005-09/2006-09在江囌省人民醫院臨床實驗研究室完成.對象:選用健康雄性SD大鼠108隻,隨機分為6組:即常氧安靜組、急性常氧高彊度組、急性常氧中彊度組、低氧安靜組、急性低練高住高彊度組和急性低練高住中彊度組,每組18隻.段氏PT型鼠跑檯為杭州產.實驗方法:急性常氧運動模型:運動前48 h進行適應活動.高彊度運動:運動50 m/min×1.5 min休2 min.中彊度運動:運動30 m/min×30 min.低氧模型:採用低氧儀產生低氧氣分壓的混閤氣體,低氧3 d,22 h/d,氧氣12.8%,溫度22℃,濕度55%.急性低練高住模型:在急性高、中彊度運動後實施上述低氧計劃.于各組處理後即刻、2,4 h分彆處死大鼠4隻取大鼠腓腸肌.主要觀察指標:用Western-blot法測定大鼠腓腸肌血管內皮生長因子錶達.結果:低氧和急性常氧運動增彊瞭血管內皮生長因子錶達,運動後低氧削弱瞭運動誘導的血管內皮生長因子錶達,長時間中等彊度的運動誘導更多的血管內皮生長因子錶達.急性運動和,或低氧後即刻、2 h血管內皮生長因子錶達最多,其恢複速度由快嚮慢排序為:低氧或低練高住、常氧運動.結論:急性運動和,或低氧誘導的大鼠骨骼肌血管內皮生長因子的錶達屬早期速髮效應,且存在彊度閾值,其恢複速度與錶達的幅度成反比,低練高住可能能增彊骨骼肌對運動的適應.
배경:통과운동화,혹저양래증가궤체대저양적응격정도화시간,가제고궤체대운동화,혹저양적괄응수평,단목전대급성운동화,혹저양대골격기혈관내피생장인자표체적영향소지심소.목적:관찰급성운동화,혹저양대대서비장기혈관내피생장인자표체적영향.설계、시간급지점:수궤대조동물실험,우2005-09/2006-09재강소성인민의원림상실험연구실완성.대상:선용건강웅성SD대서108지,수궤분위6조:즉상양안정조、급성상양고강도조、급성상양중강도조、저양안정조、급성저련고주고강도조화급성저련고주중강도조,매조18지.단씨PT형서포태위항주산.실험방법:급성상양운동모형:운동전48 h진행괄응활동.고강도운동:운동50 m/min×1.5 min휴2 min.중강도운동:운동30 m/min×30 min.저양모형:채용저양의산생저양기분압적혼합기체,저양3 d,22 h/d,양기12.8%,온도22℃,습도55%.급성저련고주모형:재급성고、중강도운동후실시상술저양계화.우각조처리후즉각、2,4 h분별처사대서4지취대서비장기.주요관찰지표:용Western-blot법측정대서비장기혈관내피생장인자표체.결과:저양화급성상양운동증강료혈관내피생장인자표체,운동후저양삭약료운동유도적혈관내피생장인자표체,장시간중등강도적운동유도경다적혈관내피생장인자표체.급성운동화,혹저양후즉각、2 h혈관내피생장인자표체최다,기회복속도유쾌향만배서위:저양혹저련고주、상양운동.결론:급성운동화,혹저양유도적대서골격기혈관내피생장인자적표체속조기속발효응,차존재강도역치,기회복속도여표체적폭도성반비,저련고주가능능증강골격기대운동적괄응.
BACKGROUND: Through exercise and/or hypoxia to increase the body's stress level and timing of hypoxia, so as to improve the body's adaptation level to exercise and/or hypoxia. However, little was known concerning the effects of acute exercise and/or hypoxia on vascular endothelial cell growth factor (VEGF) expression in skeletal muscles.OBJECTIVE: To study the effects of acute exercise and/or hypoxia on VEGF expression in rats' gastrocnemius muscles. DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at the Clinical Laboratory, People's Hospital of Jiangsu Province, between September 2005 and September 2006.MATERIALS: Totally 108 health male SD rats were randomly divided into 6 groups, namely, normoxia quiet, normoxia high intensity, normoxia moderate intensity, hypoxia quiet, living high-training low high intensity and living high-training low high intensity moderated intensity groups, with 12 animals in each group.METHODS: In acute normoxia exercise models, rats were performed adaptive activity at 48 hours prior to experiment. The high intensity exercise was comprised of 50 m/minx1.5 min training with 2 minutes rest. The moderate intensity exercise was 30 m/min×30 min. Hypoxia environment was produced by using low oxygen instrument to simulate hypoxia training, with hypoxia for 3 days, 22 h/d, 12.8% altitude, with 22 ℃ temperature and 55% humidity. In acute training low-living high models, rats were placed in above hypoxia environment after high intensity Or moderate intensity exercise. Four rats were sacrificed at hours 0, 2 and 4 after training, and the gastrocnemius muscles were obtained.MAIN OUTCOME MEASURES: The expression of VEGF in rats' gastrocnemius muscles was detected by using western-blot.RESULTS: Hypoxia and acute normoxia exercise enhanced the expression of VEGF, hypoxia after exercise weakened exercise-induced VEGF expression, and the exercise with long time and common intensity induced the higher level VEGF expression. The expression of VEGF was the most at the time points of instantaneousness and 2 hour after exercise, the sorting of the recovery speed of VEGF changes from fast to slow was: hypoxia or training low-living high and normoxic exercise. CONCLUSION: The expressions of VEGF in rats' skeletal muscles induced by acute exercise and/or hypoxia belong to the effect of immediate-early, with existing intensity-threshold, which recovery speed is inversely proportional to the expression amplitude;"training low-living high" may be able to enhance the adaptation of skeletal muscles to sports.
