CAJ | 학술논문

背景:尿毒症患者在长期透析过程中会出现与透析密切相关的淀粉样变性病症,其主要激发因子是β_2-微球蛋白,如何去除血液中β_2-微球蛋白一直是研究人员关注的重点.目的:观察不同磺化度、国产与进口来源的两种聚醚砜血液透析膜材在体外静态接触人血清时吸附去除β_2-微球蛋白的能力.方法:采用放免法测定膜材在~(125)Ⅰ-β_2-微球蛋白溶液和人血清中,于37℃孵育不同时间后对~(125)Ⅰ-β_2-微球蛋白和β_2-微球蛋白的吸附情况.结果与结论:不同来源的聚醚砜膜材对~(125)Ⅰ-β_2-微球蛋白的饱和吸附百分率由大到小依次是高磺化度聚醚砜>低磺化度聚醚砜>未磺化聚醚砜;但在血清孵育体系中,不同材料对β_2-微球蛋白的吸附均在30 min达到最大吸附值,最后稳定的吸附量由大到小依次是高磺化度聚醚砜>低磺化度聚醚砜>未磺化聚醚砜,不同来源的聚醚砜对β_2-微球蛋白的总体趋势完全一致.说明通过用膜吸附方式去除β_2-微球蛋白是一种有效的方法.对聚醚砜进行磺化改性可显著提高材料对β_2-微球蛋白的吸附去除量,而且随着磺化度增加,膜吸附β_2-微球蛋白的量增加.
배경:뇨독증환자재장기투석과정중회출현여투석밀절상관적정분양변성병증,기주요격발인자시β_2-미구단백,여하거제혈액중β_2-미구단백일직시연구인원관주적중점.목적:관찰불동광화도、국산여진구래원적량충취미풍혈액투석막재재체외정태접촉인혈청시흡부거제β_2-미구단백적능력.방법:채용방면법측정막재재~(125)Ⅰ-β_2-미구단백용액화인혈청중,우37℃부육불동시간후대~(125)Ⅰ-β_2-미구단백화β_2-미구단백적흡부정황.결과여결론:불동래원적취미풍막재대~(125)Ⅰ-β_2-미구단백적포화흡부백분솔유대도소의차시고광화도취미풍>저광화도취미풍>미광화취미풍;단재혈청부육체계중,불동재료대β_2-미구단백적흡부균재30 min체도최대흡부치,최후은정적흡부량유대도소의차시고광화도취미풍>저광화도취미풍>미광화취미풍,불동래원적취미풍대β_2-미구단백적총체추세완전일치.설명통과용막흡부방식거제β_2-미구단백시일충유효적방법.대취미풍진행광화개성가현저제고재료대β_2-미구단백적흡부거제량,이차수착광화도증가,막흡부β_2-미구단백적량증가.
BACKGROUND: Dialysis-related amyloid may occur during long-term dialysis for patients with uraemia, of which the main evocator is β_2-microglobulin (β_22M); therefore, how to eliminate 132M from blood is always the focus of research. OBJECTIVE: To observe ability of removal of β_2-microglobulin (β_2M) from serum using two kinds of polyethersulfone (PES) membrane materials with various degrees of sulfonation.METHODS: These materials were incubated in radio-labeled β_2M (~(125)Ⅰ-β_2M) solution and human serum respectively at appointed time at 37 ℃, and then the amounts of ~(125)Ⅰ-β_2M and serumβ_2M adsorbed by materials were measured by radio immunoassay. RESULTS AND CONCLUSION: In the ~(125)Ⅰ-β_2M system, amounts of ~(125)Ⅰ-β_2M adsorbed by the materials decreased in the following sequence PES with high degree of sulfonation > PES with low degree of sulfonation > PES, whatever the source of PES was. In the serum system, amounts of β_2M adsorbed reached maximums at 30 minutes and the final adsorptions decreased in sequence of PES with high degree of sulfonation > PES with low degree of sulfonaUon > PES. Sulfonated PES removed β_2M more than PES did and the adsorption of β_2M increases with the increase in the degree of sulfonation. Its ability to remove significant amount of β_2M may result in less β_2M available for incorporation into amyloid. The use of sulfonated PES membranes may lessen the likelihood of development of dialysis-related amyloidosis, which remains a major source of morbidity for patients treated with long-term hemodialysis.